A Comprehensive Analysis of Short Specific Tissue (SST) Proteins, a New Group of Proteins from PF10950 That May Give Rise to Cyclopeptide Alkaloids
Proteins of the PF10950 family feature the DUF2775 domain of unknown function. The most studied are specific tissue (ST) proteins with tandem repeats, which are putative precursors of cyclopeptide alkaloids. Here, we study uncharacterised short ST (SST) proteins with the DUFF2775 domain by analysing...
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2025-04-01
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| author | Lucía Albornos Paula Iriondo Silvia Rodríguez-Marcos Patricia Farelo Guillermo Sobrino-Mengual Luz María Muñoz-Centeno Ignacio Martín Berta Dopico |
| author_facet | Lucía Albornos Paula Iriondo Silvia Rodríguez-Marcos Patricia Farelo Guillermo Sobrino-Mengual Luz María Muñoz-Centeno Ignacio Martín Berta Dopico |
| author_sort | Lucía Albornos |
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| description | Proteins of the PF10950 family feature the DUF2775 domain of unknown function. The most studied are specific tissue (ST) proteins with tandem repeats, which are putative precursors of cyclopeptide alkaloids. Here, we study uncharacterised short ST (SST) proteins with the DUFF2775 domain by analysing 194 sequences from 120 species of 39 taxonomic families in silico. SST proteins have a signal peptide and their size and several other characteristics depend on their individual taxonomic family. Sequence analyses revealed that SST proteins contain two well-conserved regions, one resembling the ST repeat, which could constitute the core of cyclopeptide alkaloids. We studied the unique <i>SST1</i> gene of <i>Arabidopsis thaliana</i>, which is adjacent to and co-expressed with a gene encoding a protein with a BURP domain, associated with cyclopeptide production. The empirical analysis indicated that the <i>SST1</i> promoter is mainly activated in the roots, where most of the transcripts accumulate, and that the SST1 protein accumulates in the root vascular cambium. At the cellular level, SST fused to GFP appears in vesicles that co-localise with the endoplasmic reticulum and the vacuole. Thus, SSTs are a new type of PF10950 protein found in core eudicots with two conserved regions that could be involved in root biology. |
| format | Article |
| id | doaj-art-35a006704de544799cdcbdcba9efaa58 |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-35a006704de544799cdcbdcba9efaa582025-08-20T03:03:27ZengMDPI AGPlants2223-77472025-04-01147111710.3390/plants14071117A Comprehensive Analysis of Short Specific Tissue (SST) Proteins, a New Group of Proteins from PF10950 That May Give Rise to Cyclopeptide AlkaloidsLucía Albornos0Paula Iriondo1Silvia Rodríguez-Marcos2Patricia Farelo3Guillermo Sobrino-Mengual4Luz María Muñoz-Centeno5Ignacio Martín6Berta Dopico7Department of Botany and Plant Physiology, University of Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, SpainDepartment of Botany and Plant Physiology, University of Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, SpainDepartment of Botany and Plant Physiology, University of Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, SpainDepartment of Botany and Plant Physiology, University of Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, SpainDepartment of Botany and Plant Physiology, University of Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, SpainDepartment of Botany and Plant Physiology, University of Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, SpainDepartment of Botany and Plant Physiology, University of Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, SpainDepartment of Botany and Plant Physiology, University of Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, SpainProteins of the PF10950 family feature the DUF2775 domain of unknown function. The most studied are specific tissue (ST) proteins with tandem repeats, which are putative precursors of cyclopeptide alkaloids. Here, we study uncharacterised short ST (SST) proteins with the DUFF2775 domain by analysing 194 sequences from 120 species of 39 taxonomic families in silico. SST proteins have a signal peptide and their size and several other characteristics depend on their individual taxonomic family. Sequence analyses revealed that SST proteins contain two well-conserved regions, one resembling the ST repeat, which could constitute the core of cyclopeptide alkaloids. We studied the unique <i>SST1</i> gene of <i>Arabidopsis thaliana</i>, which is adjacent to and co-expressed with a gene encoding a protein with a BURP domain, associated with cyclopeptide production. The empirical analysis indicated that the <i>SST1</i> promoter is mainly activated in the roots, where most of the transcripts accumulate, and that the SST1 protein accumulates in the root vascular cambium. At the cellular level, SST fused to GFP appears in vesicles that co-localise with the endoplasmic reticulum and the vacuole. Thus, SSTs are a new type of PF10950 protein found in core eudicots with two conserved regions that could be involved in root biology.https://www.mdpi.com/2223-7747/14/7/1117DUF2775 domainSST proteinST proteinrootcyclopeptides alkaloidsPF10950 |
| spellingShingle | Lucía Albornos Paula Iriondo Silvia Rodríguez-Marcos Patricia Farelo Guillermo Sobrino-Mengual Luz María Muñoz-Centeno Ignacio Martín Berta Dopico A Comprehensive Analysis of Short Specific Tissue (SST) Proteins, a New Group of Proteins from PF10950 That May Give Rise to Cyclopeptide Alkaloids Plants DUF2775 domain SST protein ST protein root cyclopeptides alkaloids PF10950 |
| title | A Comprehensive Analysis of Short Specific Tissue (SST) Proteins, a New Group of Proteins from PF10950 That May Give Rise to Cyclopeptide Alkaloids |
| title_full | A Comprehensive Analysis of Short Specific Tissue (SST) Proteins, a New Group of Proteins from PF10950 That May Give Rise to Cyclopeptide Alkaloids |
| title_fullStr | A Comprehensive Analysis of Short Specific Tissue (SST) Proteins, a New Group of Proteins from PF10950 That May Give Rise to Cyclopeptide Alkaloids |
| title_full_unstemmed | A Comprehensive Analysis of Short Specific Tissue (SST) Proteins, a New Group of Proteins from PF10950 That May Give Rise to Cyclopeptide Alkaloids |
| title_short | A Comprehensive Analysis of Short Specific Tissue (SST) Proteins, a New Group of Proteins from PF10950 That May Give Rise to Cyclopeptide Alkaloids |
| title_sort | comprehensive analysis of short specific tissue sst proteins a new group of proteins from pf10950 that may give rise to cyclopeptide alkaloids |
| topic | DUF2775 domain SST protein ST protein root cyclopeptides alkaloids PF10950 |
| url | https://www.mdpi.com/2223-7747/14/7/1117 |
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