In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI
The magic angle effect increases the MRI signal of healthy tendon tissue and could be used for more detailed evaluation of tendon structure. Furthermore, it could support the discrimination of hypointense artefacts induced by contrast agents such as superparamagnetic iron oxide used for cell trackin...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/5670106 |
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| author | Carolin Horstmeier Annette B. Ahrberg Dagmar Berner Janina Burk Claudia Gittel Aline Hillmann Julia Offhaus Walter Brehm |
| author_facet | Carolin Horstmeier Annette B. Ahrberg Dagmar Berner Janina Burk Claudia Gittel Aline Hillmann Julia Offhaus Walter Brehm |
| author_sort | Carolin Horstmeier |
| collection | DOAJ |
| description | The magic angle effect increases the MRI signal of healthy tendon tissue and could be used for more detailed evaluation of tendon structure. Furthermore, it could support the discrimination of hypointense artefacts induced by contrast agents such as superparamagnetic iron oxide used for cell tracking. However, magic angle MRI of the equine superficial digital flexor tendon has not been accomplished in vivo in standing low-field MRI so far. The aim of this in vivo study was to evaluate the practicability of this magic angle technique and its benefit for tracking superparamagnetic iron oxide-labelled multipotent mesenchymal stromal cells. Six horses with induced tendinopathy in their forelimb superficial digital flexor tendons were injected locally either with superparamagnetic iron oxide-labelled multipotent mesenchymal stromal cells or serum. MRI included standard and magic angle image series in T1- and T2∗-weighted sequences performed at regular intervals. Image analysis comprised blinded evaluation and quantitative assessment of signal-to-noise ratio. The magic angle technique enhanced the tendon signal-to-noise ratio (P<0.001). Hypointense artefacts were observable in the cell-injected superficial digital flexor tendons over 24 weeks and artefact signal-to-noise ratio differed significantly from tendon signal-to-noise ratio in the magic angle images (P<0.001). Magic angle imaging of the equine superficial digital flexor tendon is feasible in standing low-field MRI. The current data demonstrate that the technique improves discrimination of superparamagnetic iron oxide-induced artefacts from the surrounding tendon tissue. |
| format | Article |
| id | doaj-art-35851e0b2913486784bd7cf71f3832e2 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-35851e0b2913486784bd7cf71f3832e22025-02-03T06:07:23ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/56701065670106In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRICarolin Horstmeier0Annette B. Ahrberg1Dagmar Berner2Janina Burk3Claudia Gittel4Aline Hillmann5Julia Offhaus6Walter Brehm7Department for Horses, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 21, 04103 Leipzig, GermanyDepartment of Orthopedics, Traumatology, and Plastic Surgery, University of Leipzig, Liebigstr. 20, 04103 Leipzig, GermanyRoyal Veterinary College, University of London, Hawkshead Lane, Hatfield, Hertfordshire AL9 7TA, UKEquine Clinic-Surgery, Justus Liebig University Giessen, Frankfurter Str. 108, 35392 Giessen, GermanyDepartment of Veterinary Medicine, Queen’s Veterinary School, Madingley Road, Cambridge CB3 0ES, UKSaxon Incubator for Clinical Translation, University of Leipzig, Philipp-Rosenthal-Str. 55, 04103 Leipzig, GermanyDepartment for Horses, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 21, 04103 Leipzig, GermanyDepartment for Horses, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 21, 04103 Leipzig, GermanyThe magic angle effect increases the MRI signal of healthy tendon tissue and could be used for more detailed evaluation of tendon structure. Furthermore, it could support the discrimination of hypointense artefacts induced by contrast agents such as superparamagnetic iron oxide used for cell tracking. However, magic angle MRI of the equine superficial digital flexor tendon has not been accomplished in vivo in standing low-field MRI so far. The aim of this in vivo study was to evaluate the practicability of this magic angle technique and its benefit for tracking superparamagnetic iron oxide-labelled multipotent mesenchymal stromal cells. Six horses with induced tendinopathy in their forelimb superficial digital flexor tendons were injected locally either with superparamagnetic iron oxide-labelled multipotent mesenchymal stromal cells or serum. MRI included standard and magic angle image series in T1- and T2∗-weighted sequences performed at regular intervals. Image analysis comprised blinded evaluation and quantitative assessment of signal-to-noise ratio. The magic angle technique enhanced the tendon signal-to-noise ratio (P<0.001). Hypointense artefacts were observable in the cell-injected superficial digital flexor tendons over 24 weeks and artefact signal-to-noise ratio differed significantly from tendon signal-to-noise ratio in the magic angle images (P<0.001). Magic angle imaging of the equine superficial digital flexor tendon is feasible in standing low-field MRI. The current data demonstrate that the technique improves discrimination of superparamagnetic iron oxide-induced artefacts from the surrounding tendon tissue.http://dx.doi.org/10.1155/2019/5670106 |
| spellingShingle | Carolin Horstmeier Annette B. Ahrberg Dagmar Berner Janina Burk Claudia Gittel Aline Hillmann Julia Offhaus Walter Brehm In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI Stem Cells International |
| title | In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI |
| title_full | In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI |
| title_fullStr | In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI |
| title_full_unstemmed | In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI |
| title_short | In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI |
| title_sort | in vivo magic angle magnetic resonance imaging for cell tracking in equine low field mri |
| url | http://dx.doi.org/10.1155/2019/5670106 |
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