Dendritic Cells from Aged Subjects Display Enhanced Inflammatory Responses to Chlamydophila pneumoniae
Chlamydophila pneumoniae (CPn) is a common respiratory pathogen that causes a chronic and persistent airway infection. The elderly display an increased susceptibility and severity to this infection. However, the underlying mechanisms are not well understood. Dendritic cells (DCs) are the initiators...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/436438 |
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| author | Sangeetha Prakash Sudhanshu Agrawal Dandan Ma Sudhir Gupta Ellena M. Peterson Anshu Agrawal |
| author_facet | Sangeetha Prakash Sudhanshu Agrawal Dandan Ma Sudhir Gupta Ellena M. Peterson Anshu Agrawal |
| author_sort | Sangeetha Prakash |
| collection | DOAJ |
| description | Chlamydophila pneumoniae (CPn) is a common respiratory pathogen that causes a chronic and persistent airway infection. The elderly display an increased susceptibility and severity to this infection. However, the underlying mechanisms are not well understood. Dendritic cells (DCs) are the initiators and regulators of immune responses. Therefore, we investigated the role of DCs in the age-associated increased CPn infection in vitro in humans. Though the expression of activation markers was comparable between the two age groups, DCs from aged subjects secreted enhanced levels of proinflammatory mediators such as TNF-α and CXCL-10 in response to CPn. In contrast, the secretion of IL-10 and innate interferons, IFN-α and IFN-λ, was severely impaired in DCs from aged donors. The increased activation of DCs from aged subjects to CPn also resulted in enhanced proliferation of CD4 and CD8 T cells in a DC-T coculture. Furthermore, T cells primed with CPn-stimulated DCs from aged subjects secreted increased levels of IFN-γ and reduced levels of IL-10 compared to DCs obtained from young subjects. In summary, DCs from the elderly displayed enhanced inflammatory response to CPn which may result in airway remodeling and increase the susceptibility of the elderly to respiratory diseases such as asthma. |
| format | Article |
| id | doaj-art-3584f5b767994e18bc494dbadd6fcadc |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-3584f5b767994e18bc494dbadd6fcadc2025-02-03T05:58:32ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/436438436438Dendritic Cells from Aged Subjects Display Enhanced Inflammatory Responses to Chlamydophila pneumoniaeSangeetha Prakash0Sudhanshu Agrawal1Dandan Ma2Sudhir Gupta3Ellena M. Peterson4Anshu Agrawal5Division of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA 92697, USADivision of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA 92697, USADivision of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA 92697, USADivision of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA 92697, USADepartment of Pathology and Laboratory Medicine, University of California, Irvine, CA 92697, USADivision of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA 92697, USAChlamydophila pneumoniae (CPn) is a common respiratory pathogen that causes a chronic and persistent airway infection. The elderly display an increased susceptibility and severity to this infection. However, the underlying mechanisms are not well understood. Dendritic cells (DCs) are the initiators and regulators of immune responses. Therefore, we investigated the role of DCs in the age-associated increased CPn infection in vitro in humans. Though the expression of activation markers was comparable between the two age groups, DCs from aged subjects secreted enhanced levels of proinflammatory mediators such as TNF-α and CXCL-10 in response to CPn. In contrast, the secretion of IL-10 and innate interferons, IFN-α and IFN-λ, was severely impaired in DCs from aged donors. The increased activation of DCs from aged subjects to CPn also resulted in enhanced proliferation of CD4 and CD8 T cells in a DC-T coculture. Furthermore, T cells primed with CPn-stimulated DCs from aged subjects secreted increased levels of IFN-γ and reduced levels of IL-10 compared to DCs obtained from young subjects. In summary, DCs from the elderly displayed enhanced inflammatory response to CPn which may result in airway remodeling and increase the susceptibility of the elderly to respiratory diseases such as asthma.http://dx.doi.org/10.1155/2014/436438 |
| spellingShingle | Sangeetha Prakash Sudhanshu Agrawal Dandan Ma Sudhir Gupta Ellena M. Peterson Anshu Agrawal Dendritic Cells from Aged Subjects Display Enhanced Inflammatory Responses to Chlamydophila pneumoniae Mediators of Inflammation |
| title | Dendritic Cells from Aged Subjects Display Enhanced Inflammatory Responses to Chlamydophila pneumoniae |
| title_full | Dendritic Cells from Aged Subjects Display Enhanced Inflammatory Responses to Chlamydophila pneumoniae |
| title_fullStr | Dendritic Cells from Aged Subjects Display Enhanced Inflammatory Responses to Chlamydophila pneumoniae |
| title_full_unstemmed | Dendritic Cells from Aged Subjects Display Enhanced Inflammatory Responses to Chlamydophila pneumoniae |
| title_short | Dendritic Cells from Aged Subjects Display Enhanced Inflammatory Responses to Chlamydophila pneumoniae |
| title_sort | dendritic cells from aged subjects display enhanced inflammatory responses to chlamydophila pneumoniae |
| url | http://dx.doi.org/10.1155/2014/436438 |
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