Himatanthus bracteatus stem bark ethanolic extract obtained by sequential pressurized liquid extraction: Chromatographic characterization and profiling of cytotoxic, antitumoral and immunopharmacological properties
This study aims to characterize the cytotoxic, antitumoral and immunopharmacological profile of the ethanolic extract of Himatanthus bracteatus (EEHB) stem bark. Chromatographic analysis revealed the major EEHB composition in dimethyl isoplumerideo acid, 13-deoxyplumerido, isoplumeride, and plumerid...
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Elsevier
2025-05-01
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| Series: | Journal of Traditional and Complementary Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411024000695 |
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| author | Rose N. Pereira-Filho Wilson D. Gonçalves-Júnior Agenor G. dos Santos-Neto John L.S. Cunha Oslei P. de Almeida Luciana N. Andrade Daniela Droppa-Almeida Ricardo G. Amaral Cláudio Dariva Juliana C. Cardoso Patricia Severino Eliana B. Souto Ricardo L.C. de Albuquerque-Júnior |
| author_facet | Rose N. Pereira-Filho Wilson D. Gonçalves-Júnior Agenor G. dos Santos-Neto John L.S. Cunha Oslei P. de Almeida Luciana N. Andrade Daniela Droppa-Almeida Ricardo G. Amaral Cláudio Dariva Juliana C. Cardoso Patricia Severino Eliana B. Souto Ricardo L.C. de Albuquerque-Júnior |
| author_sort | Rose N. Pereira-Filho |
| collection | DOAJ |
| description | This study aims to characterize the cytotoxic, antitumoral and immunopharmacological profile of the ethanolic extract of Himatanthus bracteatus (EEHB) stem bark. Chromatographic analysis revealed the major EEHB composition in dimethyl isoplumerideo acid, 13-deoxyplumerido, isoplumeride, and plumeride. Cytotoxicity was performed on MCF-7 and MCF-10A cell lines using MTT assay. The antitumor activity was assessed using sarcoma 180 tumor cells subcutaneously implanted in mice. After seven days, hematological and biochemical analysis, and pathological evaluation of tumors and visceral organs were carried out. The IC50 value was 28.49 ± 2.05 μg/mL on MCF7 cells, but over 320 μg/mL on MCF-10A cells. Molecular docking was predicted using the caspase 3 molecular target with plumeride and isoplumeride ligands. Both compounds were also analyzed by PreADMET. The tumor growth inhibition was comparable to 5-FU. EEHB reduced the proliferative index (Ki67 immunoexpression) but increased the expression of apoptotic markers in a sarcoma 180 model. The ligands showed interaction with Caspase 3 with a binding energy between −7.2 and −6.6 kcal/mol for isoplumeride and −7.8 to −7.0 kcal/mol for plumeride. Hydrogen interactions were present between the ligands and caspase 3. Both phytochemicals showed low or no permeability in blood-brain barrier and medium permeability in Caco-2 cells and only isoplumeride showed mutagenic potential and carcinogenic. EEHB presented no toxicological effect either on the hematological parameters or average weight and histological features of liver, kidneys, and spleen. Our data suggest that EEHB has antitumor activity in S-180 tumor-bearing mice by blocking cell cycle and increasing apoptosis. |
| format | Article |
| id | doaj-art-35820b5c123a44199e01d019b44bbe21 |
| institution | OA Journals |
| issn | 2225-4110 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
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| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj-art-35820b5c123a44199e01d019b44bbe212025-08-20T01:50:49ZengElsevierJournal of Traditional and Complementary Medicine2225-41102025-05-0115331932910.1016/j.jtcme.2024.06.004Himatanthus bracteatus stem bark ethanolic extract obtained by sequential pressurized liquid extraction: Chromatographic characterization and profiling of cytotoxic, antitumoral and immunopharmacological propertiesRose N. Pereira-Filho0Wilson D. Gonçalves-Júnior1Agenor G. dos Santos-Neto2John L.S. Cunha3Oslei P. de Almeida4Luciana N. Andrade5Daniela Droppa-Almeida6Ricardo G. Amaral7Cláudio Dariva8Juliana C. Cardoso9Patricia Severino10Eliana B. Souto11Ricardo L.C. de Albuquerque-Júnior12Research and Technology Institute, Tiradentes University, Aracaju, 49010-390, Brazil; Post-Graduating Program in Biotechnology, Brazilian Biotechnology Northeast Network (Renorbio), Tiradentes University, Aracaju, 49010-390, BrazilResearch and Technology Institute, Tiradentes University, Aracaju, 49010-390, Brazil; Post-Graduating Program in Health and Environment, Tiradentes University, Aracaju, 49032-490, BrazilPost-Graduating Program in Health and Environment, Tiradentes University, Aracaju, 49032-490, BrazilCenter of Biological and Health Sciences, Federal University of Western Bahia (UFOB), Barreiras, Bahia, 47810-047, BrazilPost-Graduating Program in Stomatopathology, University of Campinas, Campinas, São Paulo, 13083-970, BrazilDepartment of Medicine, Federal University of Sergipe, Lagarto, 49400-000, BrazilResearch and Technology Institute, Tiradentes University, Aracaju, 49010-390, BrazilDepartment of Physiology, Federal University of Sergipe, São Cristóvão, 49100-000, BrazilResearch and Technology Institute, Tiradentes University, Aracaju, 49010-390, Brazil; Post-Graduating Program in Biotechnology, Brazilian Biotechnology Northeast Network (Renorbio), Tiradentes University, Aracaju, 49010-390, Brazil; Post-Graduating Program in Process Engineering, Tiradentes University, Aracaju, 49010-390, BrazilResearch and Technology Institute, Tiradentes University, Aracaju, 49010-390, Brazil; Post-Graduating Program in Biotechnology, Brazilian Biotechnology Northeast Network (Renorbio), Tiradentes University, Aracaju, 49010-390, Brazil; Post-Graduating Program in Health and Environment, Tiradentes University, Aracaju, 49032-490, BrazilResearch and Technology Institute, Tiradentes University, Aracaju, 49010-390, Brazil; Post-Graduating Program in Biotechnology, Brazilian Biotechnology Northeast Network (Renorbio), Tiradentes University, Aracaju, 49010-390, BrazilLaboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, 4050-313, Porto, Portugal; Corresponding author. Laboratory of Pharmaceutical Technology, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.Department of Pathology, Federal University of Santa Catarina, Florianópolis, Santa Catarina, 88040-370, Brazil; Corresponding author. Department of Pathology, Federal University of Santa Catarina. R. Delfino Conti, S/N, 88040-370, Florianópolis, Santa Catarina, Brazil.This study aims to characterize the cytotoxic, antitumoral and immunopharmacological profile of the ethanolic extract of Himatanthus bracteatus (EEHB) stem bark. Chromatographic analysis revealed the major EEHB composition in dimethyl isoplumerideo acid, 13-deoxyplumerido, isoplumeride, and plumeride. Cytotoxicity was performed on MCF-7 and MCF-10A cell lines using MTT assay. The antitumor activity was assessed using sarcoma 180 tumor cells subcutaneously implanted in mice. After seven days, hematological and biochemical analysis, and pathological evaluation of tumors and visceral organs were carried out. The IC50 value was 28.49 ± 2.05 μg/mL on MCF7 cells, but over 320 μg/mL on MCF-10A cells. Molecular docking was predicted using the caspase 3 molecular target with plumeride and isoplumeride ligands. Both compounds were also analyzed by PreADMET. The tumor growth inhibition was comparable to 5-FU. EEHB reduced the proliferative index (Ki67 immunoexpression) but increased the expression of apoptotic markers in a sarcoma 180 model. The ligands showed interaction with Caspase 3 with a binding energy between −7.2 and −6.6 kcal/mol for isoplumeride and −7.8 to −7.0 kcal/mol for plumeride. Hydrogen interactions were present between the ligands and caspase 3. Both phytochemicals showed low or no permeability in blood-brain barrier and medium permeability in Caco-2 cells and only isoplumeride showed mutagenic potential and carcinogenic. EEHB presented no toxicological effect either on the hematological parameters or average weight and histological features of liver, kidneys, and spleen. Our data suggest that EEHB has antitumor activity in S-180 tumor-bearing mice by blocking cell cycle and increasing apoptosis.http://www.sciencedirect.com/science/article/pii/S2225411024000695Sequential pressurized Himatanthus bracteatus extractChromatographic analysisAntitumor drug screening assaysApoptosis |
| spellingShingle | Rose N. Pereira-Filho Wilson D. Gonçalves-Júnior Agenor G. dos Santos-Neto John L.S. Cunha Oslei P. de Almeida Luciana N. Andrade Daniela Droppa-Almeida Ricardo G. Amaral Cláudio Dariva Juliana C. Cardoso Patricia Severino Eliana B. Souto Ricardo L.C. de Albuquerque-Júnior Himatanthus bracteatus stem bark ethanolic extract obtained by sequential pressurized liquid extraction: Chromatographic characterization and profiling of cytotoxic, antitumoral and immunopharmacological properties Journal of Traditional and Complementary Medicine Sequential pressurized Himatanthus bracteatus extract Chromatographic analysis Antitumor drug screening assays Apoptosis |
| title | Himatanthus bracteatus stem bark ethanolic extract obtained by sequential pressurized liquid extraction: Chromatographic characterization and profiling of cytotoxic, antitumoral and immunopharmacological properties |
| title_full | Himatanthus bracteatus stem bark ethanolic extract obtained by sequential pressurized liquid extraction: Chromatographic characterization and profiling of cytotoxic, antitumoral and immunopharmacological properties |
| title_fullStr | Himatanthus bracteatus stem bark ethanolic extract obtained by sequential pressurized liquid extraction: Chromatographic characterization and profiling of cytotoxic, antitumoral and immunopharmacological properties |
| title_full_unstemmed | Himatanthus bracteatus stem bark ethanolic extract obtained by sequential pressurized liquid extraction: Chromatographic characterization and profiling of cytotoxic, antitumoral and immunopharmacological properties |
| title_short | Himatanthus bracteatus stem bark ethanolic extract obtained by sequential pressurized liquid extraction: Chromatographic characterization and profiling of cytotoxic, antitumoral and immunopharmacological properties |
| title_sort | himatanthus bracteatus stem bark ethanolic extract obtained by sequential pressurized liquid extraction chromatographic characterization and profiling of cytotoxic antitumoral and immunopharmacological properties |
| topic | Sequential pressurized Himatanthus bracteatus extract Chromatographic analysis Antitumor drug screening assays Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2225411024000695 |
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