Genetic susceptibility of MMP3 (rs3025058) variant allele in male football players with multiple ACL surgeries: a PCR-RFLP analysis
This study aimed to investigate the genetic susceptibility of the Matriks Metalloproteinaz-3 (MMP3) (rs3025058) gene variant allele and genotype distribution in male football players who actively participate in professional leagues and have undergone a minimum of two anterior cruciate ligament (...
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| Format: | Article |
| Language: | English |
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MRE Press
2024-10-01
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| Series: | Journal of Men's Health |
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| Online Access: | https://oss.jomh.org/files/article/20241030-409/pdf/JOMH2024031202.pdf |
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| author | Alpay Bülbül Çağatay Dereceli Meriç Eraslan Nihan Bozkurt Kübra Şahin Murat Eliöz Dila Şeyda Taşdemir Yunus Emre Güler |
| author_facet | Alpay Bülbül Çağatay Dereceli Meriç Eraslan Nihan Bozkurt Kübra Şahin Murat Eliöz Dila Şeyda Taşdemir Yunus Emre Güler |
| author_sort | Alpay Bülbül |
| collection | DOAJ |
| description | This study aimed to investigate the genetic susceptibility of the Matriks
Metalloproteinaz-3 (MMP3) (rs3025058) gene variant allele and
genotype distribution in male football players who actively participate in
professional leagues and have undergone a minimum of two anterior cruciate
ligament (ACL) surgeries. The study comprised 44 male football players who had
undergone a minimum of two ACL surgeries and 61 male football players who had not
undergone any ACL surgery. Participants’ blood samples were collected and DNA was
extracted. Allele types were determined by analyzing the MMP3
(rs3025058) variant gene polymorphism using the restriction fragment length
polymorphism (PCR-RFLP) method with appropriate primers and restriction enzymes.
The results revealed no statistical significant differences in genotype
distribution for 6A/6A, 5A/6A and 5A/5A (p = 0.736) or in 6A-allele
frequency distribution between the control (CON) and ACL groups (Odds Ratio (OR)
= 1.119, 95% Confidence Interval (CI): 0.637–1.965; p = 0.318). The
analysis of both dominant (6A/6A vs. 5A/6A + 5A/5A) and recessive (6A/6A
+ 5A/6A vs. 5A/5A) models for the inheritance of the minor allele did
not yield statistically significant difference, with odds ratios (OR) of 0.647
(95% CI: 0.283–1.478; p = 0.408) and 1.472 (95% CI: 0.506–4.281;
p = 0.584), respectively. This study investigated the impact of
MMP3 gene polymorphisms in ACL injuries, uncovering an intricate
interplay between genetic and environmental factors that contribute to injury
susceptibility. While some studies suggest potential links between MMP3
variants and ACL risks, the available information is inconclusive and
necessitates additional confirmation in other demographics. Continued research is
crucial to develop effective, personalized injury prevention strategies based on
robust genetic markers. |
| format | Article |
| id | doaj-art-357580e9a7d64d9f87c0b8edac54e73c |
| institution | OA Journals |
| issn | 1875-6867 1875-6859 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MRE Press |
| record_format | Article |
| series | Journal of Men's Health |
| spelling | doaj-art-357580e9a7d64d9f87c0b8edac54e73c2025-08-20T02:12:29ZengMRE PressJournal of Men's Health1875-68671875-68592024-10-01201018318910.22514/jomh.2024.177S1875-6867(24)00285-9Genetic susceptibility of MMP3 (rs3025058) variant allele in male football players with multiple ACL surgeries: a PCR-RFLP analysisAlpay Bülbül0Çağatay Dereceli1Meriç Eraslan2Nihan Bozkurt3Kübra Şahin4Murat Eliöz5Dila Şeyda Taşdemir6Yunus Emre Güler7Faculty of Sport Sciences, Aydın Adnan Menderes University, 09010 Aydın, TurkiyeFaculty of Sport Sciences, Aydın Adnan Menderes University, 09010 Aydın, TurkiyeFaculty of Sport Sciences, Akdenı̇z University, 07058 Antalya, TurkiyeFaculty of Medicine, Tokat Gazı̇osmanpaşa University, 60000 Tokat, TurkiyeFaculty of Medicine, Tokat Gazı̇osmanpaşa University, 60000 Tokat, TurkiyeYaşar Doğu Faculty of Sport Sciences, Ondokuz Mayıs University, 55139 Samsun, TurkiyeInstitute of Postgraduate Education, Hitit University, 19169 Çorum, TurkiyeSchool of Physical Education and Sport, İstanbul Esenyurt University, 34517 İstanbul, TurkiyeThis study aimed to investigate the genetic susceptibility of the Matriks Metalloproteinaz-3 (MMP3) (rs3025058) gene variant allele and genotype distribution in male football players who actively participate in professional leagues and have undergone a minimum of two anterior cruciate ligament (ACL) surgeries. The study comprised 44 male football players who had undergone a minimum of two ACL surgeries and 61 male football players who had not undergone any ACL surgery. Participants’ blood samples were collected and DNA was extracted. Allele types were determined by analyzing the MMP3 (rs3025058) variant gene polymorphism using the restriction fragment length polymorphism (PCR-RFLP) method with appropriate primers and restriction enzymes. The results revealed no statistical significant differences in genotype distribution for 6A/6A, 5A/6A and 5A/5A (p = 0.736) or in 6A-allele frequency distribution between the control (CON) and ACL groups (Odds Ratio (OR) = 1.119, 95% Confidence Interval (CI): 0.637–1.965; p = 0.318). The analysis of both dominant (6A/6A vs. 5A/6A + 5A/5A) and recessive (6A/6A + 5A/6A vs. 5A/5A) models for the inheritance of the minor allele did not yield statistically significant difference, with odds ratios (OR) of 0.647 (95% CI: 0.283–1.478; p = 0.408) and 1.472 (95% CI: 0.506–4.281; p = 0.584), respectively. This study investigated the impact of MMP3 gene polymorphisms in ACL injuries, uncovering an intricate interplay between genetic and environmental factors that contribute to injury susceptibility. While some studies suggest potential links between MMP3 variants and ACL risks, the available information is inconclusive and necessitates additional confirmation in other demographics. Continued research is crucial to develop effective, personalized injury prevention strategies based on robust genetic markers.https://oss.jomh.org/files/article/20241030-409/pdf/JOMH2024031202.pdfacl injuriesmatrix metalloproteinase-3gene polymorphismsmale football player |
| spellingShingle | Alpay Bülbül Çağatay Dereceli Meriç Eraslan Nihan Bozkurt Kübra Şahin Murat Eliöz Dila Şeyda Taşdemir Yunus Emre Güler Genetic susceptibility of MMP3 (rs3025058) variant allele in male football players with multiple ACL surgeries: a PCR-RFLP analysis Journal of Men's Health acl injuries matrix metalloproteinase-3 gene polymorphisms male football player |
| title | Genetic susceptibility of MMP3 (rs3025058) variant allele in
male football players with multiple ACL surgeries: a PCR-RFLP analysis |
| title_full | Genetic susceptibility of MMP3 (rs3025058) variant allele in
male football players with multiple ACL surgeries: a PCR-RFLP analysis |
| title_fullStr | Genetic susceptibility of MMP3 (rs3025058) variant allele in
male football players with multiple ACL surgeries: a PCR-RFLP analysis |
| title_full_unstemmed | Genetic susceptibility of MMP3 (rs3025058) variant allele in
male football players with multiple ACL surgeries: a PCR-RFLP analysis |
| title_short | Genetic susceptibility of MMP3 (rs3025058) variant allele in
male football players with multiple ACL surgeries: a PCR-RFLP analysis |
| title_sort | genetic susceptibility of mmp3 rs3025058 variant allele in male football players with multiple acl surgeries a pcr rflp analysis |
| topic | acl injuries matrix metalloproteinase-3 gene polymorphisms male football player |
| url | https://oss.jomh.org/files/article/20241030-409/pdf/JOMH2024031202.pdf |
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