Genetic susceptibility of MMP3 (rs3025058) variant allele in male football players with multiple ACL surgeries: a PCR-RFLP analysis
This study aimed to investigate the genetic susceptibility of the Matriks Metalloproteinaz-3 (MMP3) (rs3025058) gene variant allele and genotype distribution in male football players who actively participate in professional leagues and have undergone a minimum of two anterior cruciate ligament (...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MRE Press
2024-10-01
|
| Series: | Journal of Men's Health |
| Subjects: | |
| Online Access: | https://oss.jomh.org/files/article/20241030-409/pdf/JOMH2024031202.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This study aimed to investigate the genetic susceptibility of the Matriks
Metalloproteinaz-3 (MMP3) (rs3025058) gene variant allele and
genotype distribution in male football players who actively participate in
professional leagues and have undergone a minimum of two anterior cruciate
ligament (ACL) surgeries. The study comprised 44 male football players who had
undergone a minimum of two ACL surgeries and 61 male football players who had not
undergone any ACL surgery. Participants’ blood samples were collected and DNA was
extracted. Allele types were determined by analyzing the MMP3
(rs3025058) variant gene polymorphism using the restriction fragment length
polymorphism (PCR-RFLP) method with appropriate primers and restriction enzymes.
The results revealed no statistical significant differences in genotype
distribution for 6A/6A, 5A/6A and 5A/5A (p = 0.736) or in 6A-allele
frequency distribution between the control (CON) and ACL groups (Odds Ratio (OR)
= 1.119, 95% Confidence Interval (CI): 0.637–1.965; p = 0.318). The
analysis of both dominant (6A/6A vs. 5A/6A + 5A/5A) and recessive (6A/6A
+ 5A/6A vs. 5A/5A) models for the inheritance of the minor allele did
not yield statistically significant difference, with odds ratios (OR) of 0.647
(95% CI: 0.283–1.478; p = 0.408) and 1.472 (95% CI: 0.506–4.281;
p = 0.584), respectively. This study investigated the impact of
MMP3 gene polymorphisms in ACL injuries, uncovering an intricate
interplay between genetic and environmental factors that contribute to injury
susceptibility. While some studies suggest potential links between MMP3
variants and ACL risks, the available information is inconclusive and
necessitates additional confirmation in other demographics. Continued research is
crucial to develop effective, personalized injury prevention strategies based on
robust genetic markers. |
|---|---|
| ISSN: | 1875-6867 1875-6859 |