Analysis of Crystallinity and Morphology of Bacterial Cellulose/Chitosan Based Composite Film

Pineapple peel can be effectively utilized in fermentation process to produce bacterial cellulose (BC). This study uses pineapple peel extract to produce BC, combined with chitosan to form composite films. The study aims to analyze the crystallinity and investigate the surface morphology of BC film...

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Main Authors: Baihaqi Mukhammad Fisni, Suryanto Heru, Aminnudin Aminnudin, Puspitasari Poppy, Maulana Jibril, Nusantara Fajar, Renandito Muhammad Pramudya, Osman Azlin Fazlina, Yanuhar Uun
Format: Article
Language:English
Published: EDP Sciences 2025-01-01
Series:BIO Web of Conferences
Online Access:https://www.bio-conferences.org/articles/bioconf/pdf/2025/34/bioconf_icolist2024_01029.pdf
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author Baihaqi Mukhammad Fisni
Suryanto Heru
Aminnudin Aminnudin
Puspitasari Poppy
Maulana Jibril
Nusantara Fajar
Renandito Muhammad Pramudya
Osman Azlin Fazlina
Yanuhar Uun
author_facet Baihaqi Mukhammad Fisni
Suryanto Heru
Aminnudin Aminnudin
Puspitasari Poppy
Maulana Jibril
Nusantara Fajar
Renandito Muhammad Pramudya
Osman Azlin Fazlina
Yanuhar Uun
author_sort Baihaqi Mukhammad Fisni
collection DOAJ
description Pineapple peel can be effectively utilized in fermentation process to produce bacterial cellulose (BC). This study uses pineapple peel extract to produce BC, combined with chitosan to form composite films. The study aims to analyze the crystallinity and investigate the surface morphology of BC film with chitosan reinforcement. This method includes the cellulose synthesis through the fermentation process, alkalization, and defibrillation using high-pressure homogenization on BC and the synthesis of BC film with chitosan content of 2.5%, 5%, 7.5%, and 10% then casting and drying at 50°C for 20 hours. Crystallinity was analyzed using X-ray diffraction, and surface morphology was observed using a scanning electron microscope. Results showed an influence of adding chitosan on the surface morphology and crystallinity of the BC composite film. The diffraction pattern indicates dominant peaks with the addition of chitosan were achieved at about 11°, 14°, 16°, 22°, and 26°. The addition of chitosan reduces the crystallinity index of BC film from BC Control 83.4 % to 75.4% after adding chitosan of 10%. Surface morphology shows a smooth surface of film on BC film, but rougher after adding chitosan to make the surface corrugated. Future studies will explore the benefits of film in biopackaging applications.
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institution DOAJ
issn 2117-4458
language English
publishDate 2025-01-01
publisher EDP Sciences
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series BIO Web of Conferences
spelling doaj-art-3566f18fb2e146b5b9a8f154ec8b40d72025-08-20T03:12:46ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011830102910.1051/bioconf/202518301029bioconf_icolist2024_01029Analysis of Crystallinity and Morphology of Bacterial Cellulose/Chitosan Based Composite FilmBaihaqi Mukhammad Fisni0Suryanto Heru1Aminnudin Aminnudin2Puspitasari Poppy3Maulana Jibril4Nusantara Fajar5Renandito Muhammad Pramudya6Osman Azlin Fazlina7Yanuhar Uun8Department of Mechanical and Industrial Engineering, Faculty of Engineering, State University of MalangCenter of Excellence for Cellulose Composite (CECCom), Department of Mechanical and Industrial Engineering, Faculty of Engineering, State University of MalangDepartment of Mechanical and Industrial Engineering, Faculty of Engineering, State University of MalangDepartment of Mechanical and Industrial Engineering, Faculty of Engineering, State University of MalangDepartment of Mechanical and Industrial Engineering, Faculty of Engineering, State University of MalangDepartment of Mechanical and Industrial Engineering, Faculty of Engineering, State University of MalangDepartment of Mechanical and Industrial Engineering, Faculty of Engineering, State University of MalangFaculty of Chemical Engineering Technology, Universiti Malaysia Perlis (UniMAP)Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, Brawijaya UniversityPineapple peel can be effectively utilized in fermentation process to produce bacterial cellulose (BC). This study uses pineapple peel extract to produce BC, combined with chitosan to form composite films. The study aims to analyze the crystallinity and investigate the surface morphology of BC film with chitosan reinforcement. This method includes the cellulose synthesis through the fermentation process, alkalization, and defibrillation using high-pressure homogenization on BC and the synthesis of BC film with chitosan content of 2.5%, 5%, 7.5%, and 10% then casting and drying at 50°C for 20 hours. Crystallinity was analyzed using X-ray diffraction, and surface morphology was observed using a scanning electron microscope. Results showed an influence of adding chitosan on the surface morphology and crystallinity of the BC composite film. The diffraction pattern indicates dominant peaks with the addition of chitosan were achieved at about 11°, 14°, 16°, 22°, and 26°. The addition of chitosan reduces the crystallinity index of BC film from BC Control 83.4 % to 75.4% after adding chitosan of 10%. Surface morphology shows a smooth surface of film on BC film, but rougher after adding chitosan to make the surface corrugated. Future studies will explore the benefits of film in biopackaging applications.https://www.bio-conferences.org/articles/bioconf/pdf/2025/34/bioconf_icolist2024_01029.pdf
spellingShingle Baihaqi Mukhammad Fisni
Suryanto Heru
Aminnudin Aminnudin
Puspitasari Poppy
Maulana Jibril
Nusantara Fajar
Renandito Muhammad Pramudya
Osman Azlin Fazlina
Yanuhar Uun
Analysis of Crystallinity and Morphology of Bacterial Cellulose/Chitosan Based Composite Film
BIO Web of Conferences
title Analysis of Crystallinity and Morphology of Bacterial Cellulose/Chitosan Based Composite Film
title_full Analysis of Crystallinity and Morphology of Bacterial Cellulose/Chitosan Based Composite Film
title_fullStr Analysis of Crystallinity and Morphology of Bacterial Cellulose/Chitosan Based Composite Film
title_full_unstemmed Analysis of Crystallinity and Morphology of Bacterial Cellulose/Chitosan Based Composite Film
title_short Analysis of Crystallinity and Morphology of Bacterial Cellulose/Chitosan Based Composite Film
title_sort analysis of crystallinity and morphology of bacterial cellulose chitosan based composite film
url https://www.bio-conferences.org/articles/bioconf/pdf/2025/34/bioconf_icolist2024_01029.pdf
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