Identification and Expression Analysis of miR166 Gene Family in Response to Salt Stress in <i>Chrysanthemum</i>
cgr-miR166 was observed to be significantly enhanced in <i>Chrysanthemum</i> under 200 mM NaCl treatment. Here, ten family members were identified by aligning cgr-miR166 with scaffold sequences from the <i>Chrysanthemum nankingense</i> genome database, naming them from <i&...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/11/2/141 |
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| Summary: | cgr-miR166 was observed to be significantly enhanced in <i>Chrysanthemum</i> under 200 mM NaCl treatment. Here, ten family members were identified by aligning cgr-miR166 with scaffold sequences from the <i>Chrysanthemum nankingense</i> genome database, naming them from <i>cgr-miR166a</i> to <i>cgr-miR166j</i>, and their precursors could form stable stem-loop structures. The mature regions were observed to be highly conserved, with the 3′ end being more conserved than the 5′ end. miR166s promoters have been found to contain cis-acting elements responsive to diverse stimuli like the phytohormones ABA and IAA. qRT-RCR results demonstrated that the transcriptome sequencing results were reliable and miR166 was present at different levels in the roots, stems, leaves and flowers of <i>Chrysanthemum</i>. Furthermore, the HD-ZipIII transcription factor was validated to be the target gene of <i>Chrysanthemum</i> miR166s by degradome sequencing. Taken together, the <i>cgr-miR166</i> family exhibited both evolutionary conservation and diversification. The expression level of miR166 was upregulated in root under salt stress, while the expression level of the target gene HD-ZipIII was downregulated. These findings established the foundation for further understanding the mechanism of miR166-HD-ZipIII modules in salt response and tolerance. |
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| ISSN: | 2311-7524 |