Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches
Abstract This study proposes a tunable ink engineering methodology to allow 3D printing processability of highly bioactive but otherwise low‐viscous and unprintable blood‐derived materials. The hypothesis relies on improving the viscoelasticity and shear thinning behavior of platelet lysates (PL) an...
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| Format: | Article |
| Language: | English |
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Wiley
2024-12-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202406569 |
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| author | Rita Sobreiro‐Almeida Sara C. Santos Monize C. Decarli Marcelo Costa Tiago R. Correia Joanna Babilotte Catarina A. Custódio Lorenzo Moroni João F. Mano |
| author_facet | Rita Sobreiro‐Almeida Sara C. Santos Monize C. Decarli Marcelo Costa Tiago R. Correia Joanna Babilotte Catarina A. Custódio Lorenzo Moroni João F. Mano |
| author_sort | Rita Sobreiro‐Almeida |
| collection | DOAJ |
| description | Abstract This study proposes a tunable ink engineering methodology to allow 3D printing processability of highly bioactive but otherwise low‐viscous and unprintable blood‐derived materials. The hypothesis relies on improving the viscoelasticity and shear thinning behavior of platelet lysates (PL) and albumins (BSA) solutions by covalent coupling, enabling simultaneous extrusion and photocrosslinking upon filament deposition. The available amine groups on proteins (PL and BSA) are exploited for coupling with carboxyl groups present in methacrylated proteins (hPLMA and BSAMA), by leveraging carbodiimide chemistry. This reaction enabled the creation of a pre‐gel from these extremely low‐viscous materials (≈ 1 Pa), with precise tuning of the reaction, resulting in inks with a range of controlled viscosities and elasticities. Shape‐fidelity analysis is performed on 3D‐printed multilayered constructs, demonstrating the ability to reach clinically relevant sizes (>2 cm in size). After photocrosslinking, the scaffolds showcased a mechanically robust structure with sustained protein release over time. Bioactivity is evaluated using human adipose‐derived stem cells, resulting in increased viability and metabolic activity over time. The herein described research methodology widens the possibilities for the use of low‐viscosity materials in 3D printing but also enables the direct application of patient and blood‐derived materials in precision medicine. |
| format | Article |
| id | doaj-art-3546cbe5ed7247c5aa93abb15075dd23 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-3546cbe5ed7247c5aa93abb15075dd232024-12-18T14:18:10ZengWileyAdvanced Science2198-38442024-12-011147n/an/a10.1002/advs.202406569Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering ApproachesRita Sobreiro‐Almeida0Sara C. Santos1Monize C. Decarli2Marcelo Costa3Tiago R. Correia4Joanna Babilotte5Catarina A. Custódio6Lorenzo Moroni7João F. Mano8Department of Chemistry CICECO – Aveiro Institute of Materials University of Aveiro Campus Universitário de Santiago Aveiro 3810‐193 PortugalDepartment of Chemistry CICECO – Aveiro Institute of Materials University of Aveiro Campus Universitário de Santiago Aveiro 3810‐193 PortugalComplex Tissue Regeneration department MERLN Institute for Technology‐Inspired Regenerative Medicine Maastricht University Universiteitssingel 40 Maastricht 6229 ET The NetherlandsDepartment of Chemistry CICECO – Aveiro Institute of Materials University of Aveiro Campus Universitário de Santiago Aveiro 3810‐193 PortugalDepartment of Chemistry CICECO – Aveiro Institute of Materials University of Aveiro Campus Universitário de Santiago Aveiro 3810‐193 PortugalComplex Tissue Regeneration department MERLN Institute for Technology‐Inspired Regenerative Medicine Maastricht University Universiteitssingel 40 Maastricht 6229 ET The NetherlandsDepartment of Chemistry CICECO – Aveiro Institute of Materials University of Aveiro Campus Universitário de Santiago Aveiro 3810‐193 PortugalComplex Tissue Regeneration department MERLN Institute for Technology‐Inspired Regenerative Medicine Maastricht University Universiteitssingel 40 Maastricht 6229 ET The NetherlandsDepartment of Chemistry CICECO – Aveiro Institute of Materials University of Aveiro Campus Universitário de Santiago Aveiro 3810‐193 PortugalAbstract This study proposes a tunable ink engineering methodology to allow 3D printing processability of highly bioactive but otherwise low‐viscous and unprintable blood‐derived materials. The hypothesis relies on improving the viscoelasticity and shear thinning behavior of platelet lysates (PL) and albumins (BSA) solutions by covalent coupling, enabling simultaneous extrusion and photocrosslinking upon filament deposition. The available amine groups on proteins (PL and BSA) are exploited for coupling with carboxyl groups present in methacrylated proteins (hPLMA and BSAMA), by leveraging carbodiimide chemistry. This reaction enabled the creation of a pre‐gel from these extremely low‐viscous materials (≈ 1 Pa), with precise tuning of the reaction, resulting in inks with a range of controlled viscosities and elasticities. Shape‐fidelity analysis is performed on 3D‐printed multilayered constructs, demonstrating the ability to reach clinically relevant sizes (>2 cm in size). After photocrosslinking, the scaffolds showcased a mechanically robust structure with sustained protein release over time. Bioactivity is evaluated using human adipose‐derived stem cells, resulting in increased viability and metabolic activity over time. The herein described research methodology widens the possibilities for the use of low‐viscosity materials in 3D printing but also enables the direct application of patient and blood‐derived materials in precision medicine.https://doi.org/10.1002/advs.2024065693D printingalbuminhydrogelink engineeringphotocrosslinkingplatelet lysates |
| spellingShingle | Rita Sobreiro‐Almeida Sara C. Santos Monize C. Decarli Marcelo Costa Tiago R. Correia Joanna Babilotte Catarina A. Custódio Lorenzo Moroni João F. Mano Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches Advanced Science 3D printing albumin hydrogel ink engineering photocrosslinking platelet lysates |
| title | Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches |
| title_full | Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches |
| title_fullStr | Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches |
| title_full_unstemmed | Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches |
| title_short | Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches |
| title_sort | leveraging blood components for 3d printing applications through programmable ink engineering approaches |
| topic | 3D printing albumin hydrogel ink engineering photocrosslinking platelet lysates |
| url | https://doi.org/10.1002/advs.202406569 |
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