The Effect of Supplementing Mushroom Growing Substrates on the Bioactive Compounds, Antimicrobial Activity, and Antioxidant Activity of Pleurotus ostreatus
Pleurotus ostreatus mushroom contains important bioactive compounds and has several biological activities; however, mushroom growing substrates have major influence on chemical and functional characteristics of the mushroom. Hence, the study aimed to evaluate the influence of supplementing mushroom...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2022/9436614 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832565704142880768 |
|---|---|
| author | Senzosenkosi Surprise Mkhize Mthokozisi Blessing Cedric Simelane Ishmael Nkoana Mongalo Ofentse Jacob Pooe |
| author_facet | Senzosenkosi Surprise Mkhize Mthokozisi Blessing Cedric Simelane Ishmael Nkoana Mongalo Ofentse Jacob Pooe |
| author_sort | Senzosenkosi Surprise Mkhize |
| collection | DOAJ |
| description | Pleurotus ostreatus mushroom contains important bioactive compounds and has several biological activities; however, mushroom growing substrates have major influence on chemical and functional characteristics of the mushroom. Hence, the study aimed to evaluate the influence of supplementing mushroom growing substrates with wheat bran (WB) towards yield/productivity, bioactive compounds, and antimicrobial and antioxidant activity of P. ostreatus. The mushroom was cultivated on sugarcane substrates supplemented with increasing levels of WB (0%–20%). The mushroom extracts were screened for bioactive compounds using gas chromatography-mass spectrometry (GC-MS). Antimicrobial activity was carried out using microplate assay, while antioxidant potential was investigated using reducing power assay. The addition of supplements on mushroom growing substrates had an influence on mushroom yield; hence, higher supplementation (18% and 20%) produced higher yield. The GC-MS revealed several bioactive compounds with known activity, such as vitamin E, phenol, fatty acids, and terpenoids. Concentration-dependent antioxidant activity was observed; hence, extracts at higher concentrations gave significantly higher reducing power. The P. ostreatus extract had antimicrobial activity against all the tested organisms, with S. aureus showing high susceptibility to most of the extracts. However, mushrooms grown on bagasse substrates supplemented with 14% (0.02 mg/ml) and 20% WB (0.08 mg/ml) proved to have better antimicrobial activity on Escherichia coli. The difference in susceptibility demonstrates that substrates type and composition could have an influence on bioactive compounds found within mushrooms, also influencing medicinal properties of edible mushroom. Thus, supplementing mushroom growing substrates not only improve yield, but also can contribute to bioactive compounds with medicinal potential. |
| format | Article |
| id | doaj-art-3543eb1d0509418484e1463bdf608059 |
| institution | Kabale University |
| issn | 2090-2255 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-3543eb1d0509418484e1463bdf6080592025-02-03T01:06:46ZengWileyBiochemistry Research International2090-22552022-01-01202210.1155/2022/9436614The Effect of Supplementing Mushroom Growing Substrates on the Bioactive Compounds, Antimicrobial Activity, and Antioxidant Activity of Pleurotus ostreatusSenzosenkosi Surprise Mkhize0Mthokozisi Blessing Cedric Simelane1Ishmael Nkoana Mongalo2Ofentse Jacob Pooe3Discipline of BiochemistryDepartment of BiochemistryCollege of Agriculture and Environmental Science (CAES) LaboratoriesDiscipline of BiochemistryPleurotus ostreatus mushroom contains important bioactive compounds and has several biological activities; however, mushroom growing substrates have major influence on chemical and functional characteristics of the mushroom. Hence, the study aimed to evaluate the influence of supplementing mushroom growing substrates with wheat bran (WB) towards yield/productivity, bioactive compounds, and antimicrobial and antioxidant activity of P. ostreatus. The mushroom was cultivated on sugarcane substrates supplemented with increasing levels of WB (0%–20%). The mushroom extracts were screened for bioactive compounds using gas chromatography-mass spectrometry (GC-MS). Antimicrobial activity was carried out using microplate assay, while antioxidant potential was investigated using reducing power assay. The addition of supplements on mushroom growing substrates had an influence on mushroom yield; hence, higher supplementation (18% and 20%) produced higher yield. The GC-MS revealed several bioactive compounds with known activity, such as vitamin E, phenol, fatty acids, and terpenoids. Concentration-dependent antioxidant activity was observed; hence, extracts at higher concentrations gave significantly higher reducing power. The P. ostreatus extract had antimicrobial activity against all the tested organisms, with S. aureus showing high susceptibility to most of the extracts. However, mushrooms grown on bagasse substrates supplemented with 14% (0.02 mg/ml) and 20% WB (0.08 mg/ml) proved to have better antimicrobial activity on Escherichia coli. The difference in susceptibility demonstrates that substrates type and composition could have an influence on bioactive compounds found within mushrooms, also influencing medicinal properties of edible mushroom. Thus, supplementing mushroom growing substrates not only improve yield, but also can contribute to bioactive compounds with medicinal potential.http://dx.doi.org/10.1155/2022/9436614 |
| spellingShingle | Senzosenkosi Surprise Mkhize Mthokozisi Blessing Cedric Simelane Ishmael Nkoana Mongalo Ofentse Jacob Pooe The Effect of Supplementing Mushroom Growing Substrates on the Bioactive Compounds, Antimicrobial Activity, and Antioxidant Activity of Pleurotus ostreatus Biochemistry Research International |
| title | The Effect of Supplementing Mushroom Growing Substrates on the Bioactive Compounds, Antimicrobial Activity, and Antioxidant Activity of Pleurotus ostreatus |
| title_full | The Effect of Supplementing Mushroom Growing Substrates on the Bioactive Compounds, Antimicrobial Activity, and Antioxidant Activity of Pleurotus ostreatus |
| title_fullStr | The Effect of Supplementing Mushroom Growing Substrates on the Bioactive Compounds, Antimicrobial Activity, and Antioxidant Activity of Pleurotus ostreatus |
| title_full_unstemmed | The Effect of Supplementing Mushroom Growing Substrates on the Bioactive Compounds, Antimicrobial Activity, and Antioxidant Activity of Pleurotus ostreatus |
| title_short | The Effect of Supplementing Mushroom Growing Substrates on the Bioactive Compounds, Antimicrobial Activity, and Antioxidant Activity of Pleurotus ostreatus |
| title_sort | effect of supplementing mushroom growing substrates on the bioactive compounds antimicrobial activity and antioxidant activity of pleurotus ostreatus |
| url | http://dx.doi.org/10.1155/2022/9436614 |
| work_keys_str_mv | AT senzosenkosisurprisemkhize theeffectofsupplementingmushroomgrowingsubstratesonthebioactivecompoundsantimicrobialactivityandantioxidantactivityofpleurotusostreatus AT mthokozisiblessingcedricsimelane theeffectofsupplementingmushroomgrowingsubstratesonthebioactivecompoundsantimicrobialactivityandantioxidantactivityofpleurotusostreatus AT ishmaelnkoanamongalo theeffectofsupplementingmushroomgrowingsubstratesonthebioactivecompoundsantimicrobialactivityandantioxidantactivityofpleurotusostreatus AT ofentsejacobpooe theeffectofsupplementingmushroomgrowingsubstratesonthebioactivecompoundsantimicrobialactivityandantioxidantactivityofpleurotusostreatus AT senzosenkosisurprisemkhize effectofsupplementingmushroomgrowingsubstratesonthebioactivecompoundsantimicrobialactivityandantioxidantactivityofpleurotusostreatus AT mthokozisiblessingcedricsimelane effectofsupplementingmushroomgrowingsubstratesonthebioactivecompoundsantimicrobialactivityandantioxidantactivityofpleurotusostreatus AT ishmaelnkoanamongalo effectofsupplementingmushroomgrowingsubstratesonthebioactivecompoundsantimicrobialactivityandantioxidantactivityofpleurotusostreatus AT ofentsejacobpooe effectofsupplementingmushroomgrowingsubstratesonthebioactivecompoundsantimicrobialactivityandantioxidantactivityofpleurotusostreatus |