Small extracellular vesicles contain metals and transfer metal intercellularly
Abstract Cells have developed a highly regulated system for the uptake, transport, utilization, storage, and export of metals, ensuring the maintenance of cellular homeostasis. Small extracellular vesicles (sEVs) function as a mechanism through which a cell can export its cargo and transfer it to re...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-10-01
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| Series: | Journal of Extracellular Biology |
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| Online Access: | https://doi.org/10.1002/jex2.70012 |
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| author | Adityas Purnianto Celeste Mawal Mitali M. Kulkarni Huaqi Su Tiana F. Koukoulis Patricia Wongsodirdjo Ya Hui Hung Scott Ayton Ashley I. Bush Kevin J. Barnham Laura J. Vella |
| author_facet | Adityas Purnianto Celeste Mawal Mitali M. Kulkarni Huaqi Su Tiana F. Koukoulis Patricia Wongsodirdjo Ya Hui Hung Scott Ayton Ashley I. Bush Kevin J. Barnham Laura J. Vella |
| author_sort | Adityas Purnianto |
| collection | DOAJ |
| description | Abstract Cells have developed a highly regulated system for the uptake, transport, utilization, storage, and export of metals, ensuring the maintenance of cellular homeostasis. Small extracellular vesicles (sEVs) function as a mechanism through which a cell can export its cargo and transfer it to recipient cells. However, in contrast to the other molecular cargo associated with sEVs, the metal content of sEVs is not well characterized. To address this gap in knowledge, we measured the levels of nine essential metals (copper, iron, zinc, manganese, magnesium, potassium, calcium, chromium, cobalt) and six non‐essential metals (nickel, rubidium, titanium, aluminium, lithium, lead) in sEVs originating from multiple in vitro and ex vivo sources. Our findings reveal that, beyond containing redox‐active essential metals and those involved in redox reactions, sEVs also exhibit the capability to export and transfer non‐physiological, potentially toxic metals. |
| format | Article |
| id | doaj-art-354283fce5264150957df1fa668a3d24 |
| institution | OA Journals |
| issn | 2768-2811 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Biology |
| spelling | doaj-art-354283fce5264150957df1fa668a3d242025-08-20T02:12:03ZengWileyJournal of Extracellular Biology2768-28112024-10-01310n/an/a10.1002/jex2.70012Small extracellular vesicles contain metals and transfer metal intercellularlyAdityas Purnianto0Celeste Mawal1Mitali M. Kulkarni2Huaqi Su3Tiana F. Koukoulis4Patricia Wongsodirdjo5Ya Hui Hung6Scott Ayton7Ashley I. Bush8Kevin J. Barnham9Laura J. Vella10The Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria AustraliaThe Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria AustraliaThe Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria AustraliaThe Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria AustraliaThe Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria AustraliaThe Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria AustraliaThe Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria AustraliaThe Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria AustraliaThe Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria AustraliaThe Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria AustraliaThe Florey Institute of Neuroscience and Mental Health The University of Melbourne Parkville Victoria AustraliaAbstract Cells have developed a highly regulated system for the uptake, transport, utilization, storage, and export of metals, ensuring the maintenance of cellular homeostasis. Small extracellular vesicles (sEVs) function as a mechanism through which a cell can export its cargo and transfer it to recipient cells. However, in contrast to the other molecular cargo associated with sEVs, the metal content of sEVs is not well characterized. To address this gap in knowledge, we measured the levels of nine essential metals (copper, iron, zinc, manganese, magnesium, potassium, calcium, chromium, cobalt) and six non‐essential metals (nickel, rubidium, titanium, aluminium, lithium, lead) in sEVs originating from multiple in vitro and ex vivo sources. Our findings reveal that, beyond containing redox‐active essential metals and those involved in redox reactions, sEVs also exhibit the capability to export and transfer non‐physiological, potentially toxic metals.https://doi.org/10.1002/jex2.70012exosomesintercellular transfermetalsmall extracellular vesicles |
| spellingShingle | Adityas Purnianto Celeste Mawal Mitali M. Kulkarni Huaqi Su Tiana F. Koukoulis Patricia Wongsodirdjo Ya Hui Hung Scott Ayton Ashley I. Bush Kevin J. Barnham Laura J. Vella Small extracellular vesicles contain metals and transfer metal intercellularly Journal of Extracellular Biology exosomes intercellular transfer metal small extracellular vesicles |
| title | Small extracellular vesicles contain metals and transfer metal intercellularly |
| title_full | Small extracellular vesicles contain metals and transfer metal intercellularly |
| title_fullStr | Small extracellular vesicles contain metals and transfer metal intercellularly |
| title_full_unstemmed | Small extracellular vesicles contain metals and transfer metal intercellularly |
| title_short | Small extracellular vesicles contain metals and transfer metal intercellularly |
| title_sort | small extracellular vesicles contain metals and transfer metal intercellularly |
| topic | exosomes intercellular transfer metal small extracellular vesicles |
| url | https://doi.org/10.1002/jex2.70012 |
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