Multiple low-dose radiation ameliorates type-2 diabetes mellitus via gut microbiota modulation to activate TLR4/MyD88/NF-κB pathway
Abstract Background Type 2 diabetes mellitus (T2DM) is the fastest-growing metabolic disease in the world. The gut microbiota is linked to T2DM. Recent studies have showed that the metabolism of gut microbiota can trigger T2DM. Low dose radiation (LDR) has been proved to activate various protective...
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BMC
2025-02-01
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| Series: | BMC Endocrine Disorders |
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| Online Access: | https://doi.org/10.1186/s12902-025-01861-z |
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| author | Lijing Qin Rongrong Liu Zhen Jia Weiqiang Xu Li Wang Hongyuan Tian Xinru Lian Wen Li Yali Qi Huan He Zhicheng Wang |
| author_facet | Lijing Qin Rongrong Liu Zhen Jia Weiqiang Xu Li Wang Hongyuan Tian Xinru Lian Wen Li Yali Qi Huan He Zhicheng Wang |
| author_sort | Lijing Qin |
| collection | DOAJ |
| description | Abstract Background Type 2 diabetes mellitus (T2DM) is the fastest-growing metabolic disease in the world. The gut microbiota is linked to T2DM. Recent studies have showed that the metabolism of gut microbiota can trigger T2DM. Low dose radiation (LDR) has been proved to activate various protective bioeffects on diabetes. However, the underlying mechanisms remain unclear. Methods In this study, T2DM model was established using high fat diet combined with streptozocin (STZ) injection in C57BL/6 mice, and then exposed to multiple 75 mGy LDR every other day for one month. The changes of blood glucose levels, body weight, and the damage of pancreas were measured. In addition, 16 S rDNA amplicon sequencing was used to detect gut microbiota alteration. Metabolic profiling was carried out using the liquid mass spectrometry system, followed by the combinative analysis of gut microbiota alteration. Furthermore, the inflammatory factors and related pathways were detected. Results We found that LDR attenuate blood glucose levels and the weights of body in T2DM mice, and reduce pancreas impairment. In addition, in the gut, LDR regulated the relative abundance of Bacilli, Desulfobacterota, Verrucomicrobiota, and Proteobacteria. The non-target metabolomics analysis found that LDR significantly improve the metabolic abnormalities in T2DM, which is closely related to the gut microbiota abundance. Furthermore, the inflammatory effects activated by TLR4/MyD88/NF-κB pathways in T2DM were ameliorated by LDR. Conclusion These results suggest that LDR may exert a beneficial role in T2DM by modulating gut microbiota and metabolites, especially in TLR4/MyD88/NF-κB pathway. |
| format | Article |
| id | doaj-art-35294e8d0e984d17ba78c850b06f75e1 |
| institution | Kabale University |
| issn | 1472-6823 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Endocrine Disorders |
| spelling | doaj-art-35294e8d0e984d17ba78c850b06f75e12025-02-09T12:41:12ZengBMCBMC Endocrine Disorders1472-68232025-02-0125111110.1186/s12902-025-01861-zMultiple low-dose radiation ameliorates type-2 diabetes mellitus via gut microbiota modulation to activate TLR4/MyD88/NF-κB pathwayLijing Qin0Rongrong Liu1Zhen Jia2Weiqiang Xu3Li Wang4Hongyuan Tian5Xinru Lian6Wen Li7Yali Qi8Huan He9Zhicheng Wang10NHC Key Laboratory of Radiobiology, School of Public Health, Jilin UniversityNHC Key Laboratory of Radiobiology, School of Public Health, Jilin UniversityNHC Key Laboratory of Radiobiology, School of Public Health, Jilin UniversityNHC Key Laboratory of Radiobiology, School of Public Health, Jilin UniversityNHC Key Laboratory of Radiobiology, School of Public Health, Jilin UniversityNHC Key Laboratory of Radiobiology, School of Public Health, Jilin UniversityNHC Key Laboratory of Radiobiology, School of Public Health, Jilin UniversityNHC Key Laboratory of Radiobiology, School of Public Health, Jilin UniversityJilin Medical UniversityNHC Key Laboratory of Radiobiology, School of Public Health, Jilin UniversityNHC Key Laboratory of Radiobiology, School of Public Health, Jilin UniversityAbstract Background Type 2 diabetes mellitus (T2DM) is the fastest-growing metabolic disease in the world. The gut microbiota is linked to T2DM. Recent studies have showed that the metabolism of gut microbiota can trigger T2DM. Low dose radiation (LDR) has been proved to activate various protective bioeffects on diabetes. However, the underlying mechanisms remain unclear. Methods In this study, T2DM model was established using high fat diet combined with streptozocin (STZ) injection in C57BL/6 mice, and then exposed to multiple 75 mGy LDR every other day for one month. The changes of blood glucose levels, body weight, and the damage of pancreas were measured. In addition, 16 S rDNA amplicon sequencing was used to detect gut microbiota alteration. Metabolic profiling was carried out using the liquid mass spectrometry system, followed by the combinative analysis of gut microbiota alteration. Furthermore, the inflammatory factors and related pathways were detected. Results We found that LDR attenuate blood glucose levels and the weights of body in T2DM mice, and reduce pancreas impairment. In addition, in the gut, LDR regulated the relative abundance of Bacilli, Desulfobacterota, Verrucomicrobiota, and Proteobacteria. The non-target metabolomics analysis found that LDR significantly improve the metabolic abnormalities in T2DM, which is closely related to the gut microbiota abundance. Furthermore, the inflammatory effects activated by TLR4/MyD88/NF-κB pathways in T2DM were ameliorated by LDR. Conclusion These results suggest that LDR may exert a beneficial role in T2DM by modulating gut microbiota and metabolites, especially in TLR4/MyD88/NF-κB pathway.https://doi.org/10.1186/s12902-025-01861-zType 2 diabetes mellitusLow dose radiationGut microbiotaMetabolitesToll-like receptor 4 |
| spellingShingle | Lijing Qin Rongrong Liu Zhen Jia Weiqiang Xu Li Wang Hongyuan Tian Xinru Lian Wen Li Yali Qi Huan He Zhicheng Wang Multiple low-dose radiation ameliorates type-2 diabetes mellitus via gut microbiota modulation to activate TLR4/MyD88/NF-κB pathway BMC Endocrine Disorders Type 2 diabetes mellitus Low dose radiation Gut microbiota Metabolites Toll-like receptor 4 |
| title | Multiple low-dose radiation ameliorates type-2 diabetes mellitus via gut microbiota modulation to activate TLR4/MyD88/NF-κB pathway |
| title_full | Multiple low-dose radiation ameliorates type-2 diabetes mellitus via gut microbiota modulation to activate TLR4/MyD88/NF-κB pathway |
| title_fullStr | Multiple low-dose radiation ameliorates type-2 diabetes mellitus via gut microbiota modulation to activate TLR4/MyD88/NF-κB pathway |
| title_full_unstemmed | Multiple low-dose radiation ameliorates type-2 diabetes mellitus via gut microbiota modulation to activate TLR4/MyD88/NF-κB pathway |
| title_short | Multiple low-dose radiation ameliorates type-2 diabetes mellitus via gut microbiota modulation to activate TLR4/MyD88/NF-κB pathway |
| title_sort | multiple low dose radiation ameliorates type 2 diabetes mellitus via gut microbiota modulation to activate tlr4 myd88 nf κb pathway |
| topic | Type 2 diabetes mellitus Low dose radiation Gut microbiota Metabolites Toll-like receptor 4 |
| url | https://doi.org/10.1186/s12902-025-01861-z |
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