Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats
Organ/tissue transplantation has become an effective therapy for end-stage diseases. However, immunosuppression after transplantation may cause severe side effects. Donor-specific transplant tolerance was proposed to solve this problem. In this study, we report a novel method for inducing and mainta...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/483856 |
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| _version_ | 1849305890975383552 |
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| author | Quan Liu Yong Wang Atsunori Nakao Wensheng Zhang Vijay Gorantla Xin Xiao Zheng |
| author_facet | Quan Liu Yong Wang Atsunori Nakao Wensheng Zhang Vijay Gorantla Xin Xiao Zheng |
| author_sort | Quan Liu |
| collection | DOAJ |
| description | Organ/tissue transplantation has become an effective therapy for end-stage diseases. However, immunosuppression after transplantation may cause severe side effects. Donor-specific transplant tolerance was proposed to solve this problem. In this study, we report a novel method for inducing and maintaining heart allograft tolerance rats. First, we induced indefinite vascularized hind-limb allograft survival with a short-term antilymphocyte serum + Cyclosporine A treatment. Peripheral blood chimerism disappeared 6-7 weeks after immunosuppression was withdrawn. Then the recipients accepted secondary donor-strain skin and heart transplantation 200 days following vascularized hind-limb transplantation without any immunosuppression, but rejected third party skin allografts, a status of donor-specific tolerance. The ELISPOT results suggested a mechanism of clone deletion. These findings open new perspectives for the role of vascularized hind-limb transplant in the induction and maintenance of organ transplantation tolerance. |
| format | Article |
| id | doaj-art-3525a5e33dfb4a1a9041ff807cb9c2d4 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-3525a5e33dfb4a1a9041ff807cb9c2d42025-08-20T03:55:16ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/483856483856Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in RatsQuan Liu0Yong Wang1Atsunori Nakao2Wensheng Zhang3Vijay Gorantla4Xin Xiao Zheng5Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USAThomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USAThomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USAThomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USADepartment of Plastic and Reconstructive Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USAThomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USAOrgan/tissue transplantation has become an effective therapy for end-stage diseases. However, immunosuppression after transplantation may cause severe side effects. Donor-specific transplant tolerance was proposed to solve this problem. In this study, we report a novel method for inducing and maintaining heart allograft tolerance rats. First, we induced indefinite vascularized hind-limb allograft survival with a short-term antilymphocyte serum + Cyclosporine A treatment. Peripheral blood chimerism disappeared 6-7 weeks after immunosuppression was withdrawn. Then the recipients accepted secondary donor-strain skin and heart transplantation 200 days following vascularized hind-limb transplantation without any immunosuppression, but rejected third party skin allografts, a status of donor-specific tolerance. The ELISPOT results suggested a mechanism of clone deletion. These findings open new perspectives for the role of vascularized hind-limb transplant in the induction and maintenance of organ transplantation tolerance.http://dx.doi.org/10.1155/2013/483856 |
| spellingShingle | Quan Liu Yong Wang Atsunori Nakao Wensheng Zhang Vijay Gorantla Xin Xiao Zheng Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats Clinical and Developmental Immunology |
| title | Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats |
| title_full | Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats |
| title_fullStr | Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats |
| title_full_unstemmed | Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats |
| title_short | Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats |
| title_sort | heart allograft tolerance induced and maintained by vascularized hind limb transplant in rats |
| url | http://dx.doi.org/10.1155/2013/483856 |
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