Identification and functional analysis of hub genes involved in deoxynivalenol-induced enterotoxicity in porcine (Sus scrofa)
Deoxynivalenol (DON) is a type of mycotoxin commonly found in food and animal feed. When consumed, it can have harmful effects on the intestine. The porcine digestive system is physiologically similar to that of humans, making pigs a suitable model for studying DON-induced enterotoxicity. However, t...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016208 |
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| author | Jinhua He Geng Zhao Mingxia Chen Ximing Ren Peizhi Zhu Zhizhong Liu Jiayi Zhou Hanwei Chen Chuqiao Xiao Xiang-Guang Li |
| author_facet | Jinhua He Geng Zhao Mingxia Chen Ximing Ren Peizhi Zhu Zhizhong Liu Jiayi Zhou Hanwei Chen Chuqiao Xiao Xiang-Guang Li |
| author_sort | Jinhua He |
| collection | DOAJ |
| description | Deoxynivalenol (DON) is a type of mycotoxin commonly found in food and animal feed. When consumed, it can have harmful effects on the intestine. The porcine digestive system is physiologically similar to that of humans, making pigs a suitable model for studying DON-induced enterotoxicity. However, the exact ways DON causes intestinal damage in pigs still need to be fully understood. To address this knowledge gap, this study aimed to identify hub genes associated with enterotoxicity caused by DON exposure. Transcriptomic datasets from porcine jejunal explants exposed to DON were extensively analyzed using bioinformatic techniques in this study. A total of 265 differentially expressed genes (DEGs) were identified, with 238 being up-regulated and 27 being down-regulated, indicating that exposure to DON tends to increase gene expression. Further analysis revealed that the up-regulated DEGs were enriched in tumor necrosis factor, nuclear factor kappa-B, mitogen-activated protein kinases, and Janus kinase/signal transducer and activator of transcription-related signaling pathways. In addition, Weighted gene co-expression network analysis was performed to identify highly co-expressed modules. Then, genes in the highest co-expressed module were intersected with the up-regulated DEGs to construct a Protein-Protein Interaction network, resulting in 237 overlapping genes. Subsequently, 6 hub genes (CXCR4, PTGS2, ICAM1, IL-1A, IL-1B, and IL-10) that played a central role in the response to DON were identified using cytohubba in conjunction with the Molecular Complex Detection. In summary, exposure to DON is more likely to result in increased rather than decreased gene expression. Six of the upregulated genes, which are involved in immunoregulation and inflammation, were identified as hub genes related to DON-induced enterotoxicity in pigs. This study provides new insights into the mechanisms underlying DON-induced enterotoxicity and could guide interventions for this condition. |
| format | Article |
| id | doaj-art-350aa336f0f446d69927e150481c7911 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-350aa336f0f446d69927e150481c79112025-02-12T05:29:37ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117544Identification and functional analysis of hub genes involved in deoxynivalenol-induced enterotoxicity in porcine (Sus scrofa)Jinhua He0Geng Zhao1Mingxia Chen2Ximing Ren3Peizhi Zhu4Zhizhong Liu5Jiayi Zhou6Hanwei Chen7Chuqiao Xiao8Xiang-Guang Li9Central Laboratory, The Affiliated Panyu Central Hospital of Guangzhou Medical University, Guangzhou 511400, ChinaCentral Laboratory, The Affiliated Panyu Central Hospital of Guangzhou Medical University, Guangzhou 511400, China; Department of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaSchool of Animal Science and Technology, Guangdong Polytechnic of Science and Trade, Qingyuan 511500, ChinaDepartment of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaDepartment of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaCentral Laboratory, The Affiliated Panyu Central Hospital of Guangzhou Medical University, Guangzhou 511400, China; Department of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaCentral Laboratory, The Affiliated Panyu Central Hospital of Guangzhou Medical University, Guangzhou 511400, China; Panyu Health Management Center (Panyu Rehabilitation Hospital), Guangzhou 511450, ChinaDepartment of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China; Corresponding authors.Department of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China; Corresponding authors.Deoxynivalenol (DON) is a type of mycotoxin commonly found in food and animal feed. When consumed, it can have harmful effects on the intestine. The porcine digestive system is physiologically similar to that of humans, making pigs a suitable model for studying DON-induced enterotoxicity. However, the exact ways DON causes intestinal damage in pigs still need to be fully understood. To address this knowledge gap, this study aimed to identify hub genes associated with enterotoxicity caused by DON exposure. Transcriptomic datasets from porcine jejunal explants exposed to DON were extensively analyzed using bioinformatic techniques in this study. A total of 265 differentially expressed genes (DEGs) were identified, with 238 being up-regulated and 27 being down-regulated, indicating that exposure to DON tends to increase gene expression. Further analysis revealed that the up-regulated DEGs were enriched in tumor necrosis factor, nuclear factor kappa-B, mitogen-activated protein kinases, and Janus kinase/signal transducer and activator of transcription-related signaling pathways. In addition, Weighted gene co-expression network analysis was performed to identify highly co-expressed modules. Then, genes in the highest co-expressed module were intersected with the up-regulated DEGs to construct a Protein-Protein Interaction network, resulting in 237 overlapping genes. Subsequently, 6 hub genes (CXCR4, PTGS2, ICAM1, IL-1A, IL-1B, and IL-10) that played a central role in the response to DON were identified using cytohubba in conjunction with the Molecular Complex Detection. In summary, exposure to DON is more likely to result in increased rather than decreased gene expression. Six of the upregulated genes, which are involved in immunoregulation and inflammation, were identified as hub genes related to DON-induced enterotoxicity in pigs. This study provides new insights into the mechanisms underlying DON-induced enterotoxicity and could guide interventions for this condition.http://www.sciencedirect.com/science/article/pii/S0147651324016208DeoxynivalenolEnterotoxicityInterleukinChemokineInflammationNetwork analysis |
| spellingShingle | Jinhua He Geng Zhao Mingxia Chen Ximing Ren Peizhi Zhu Zhizhong Liu Jiayi Zhou Hanwei Chen Chuqiao Xiao Xiang-Guang Li Identification and functional analysis of hub genes involved in deoxynivalenol-induced enterotoxicity in porcine (Sus scrofa) Ecotoxicology and Environmental Safety Deoxynivalenol Enterotoxicity Interleukin Chemokine Inflammation Network analysis |
| title | Identification and functional analysis of hub genes involved in deoxynivalenol-induced enterotoxicity in porcine (Sus scrofa) |
| title_full | Identification and functional analysis of hub genes involved in deoxynivalenol-induced enterotoxicity in porcine (Sus scrofa) |
| title_fullStr | Identification and functional analysis of hub genes involved in deoxynivalenol-induced enterotoxicity in porcine (Sus scrofa) |
| title_full_unstemmed | Identification and functional analysis of hub genes involved in deoxynivalenol-induced enterotoxicity in porcine (Sus scrofa) |
| title_short | Identification and functional analysis of hub genes involved in deoxynivalenol-induced enterotoxicity in porcine (Sus scrofa) |
| title_sort | identification and functional analysis of hub genes involved in deoxynivalenol induced enterotoxicity in porcine sus scrofa |
| topic | Deoxynivalenol Enterotoxicity Interleukin Chemokine Inflammation Network analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016208 |
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