Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1
Laminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a significant association between anti-laminin-1 antibodies (Abs...
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| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2005-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520400014168 |
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| author | Akane Kondo Junko Inagaki Kazuko Kobayashi Hideo Tsukamoto Daisuke Yamamoto Mikiya Nakatsuka Nobuharu Suzuki Motoyoshi Nomizu Satoshi Amano Hidehiko Matsubayashi Tatsuji Yasuda Luis R. Lopez Yehuda Shoenfeld Tsunehisa Makino Eiji Matsuura |
| author_facet | Akane Kondo Junko Inagaki Kazuko Kobayashi Hideo Tsukamoto Daisuke Yamamoto Mikiya Nakatsuka Nobuharu Suzuki Motoyoshi Nomizu Satoshi Amano Hidehiko Matsubayashi Tatsuji Yasuda Luis R. Lopez Yehuda Shoenfeld Tsunehisa Makino Eiji Matsuura |
| author_sort | Akane Kondo |
| collection | DOAJ |
| description | Laminin-1 is a structural glycoprotein that forms an integral part of
the scaffolding of basement membranes, and plays an important role during
embryonic development. We have recently demonstrated a significant association
between anti-laminin-1 antibodies (Abs) and reproductive failure, such as recurrent
spontaneous abortions and infertility-associated endometriosis in both human and
mouse studies. In the present study, we established an IgM (μ,κ) monoclonal
anti-laminin-1 Ab (AK8) by immunizing mice with mouse Engelbreth-Holm-Swarm
sarcoma (EHS)-derived laminin-α1. The AK8 monoclonal antibody (mAb) reacted with
particular peptide sequences from the globular G domain of mouse laminin-α1 chain
of using ELISA and Western blot techniques. The peptide tertiary structure of
the epitope recognized by AK8 mAb was predicted using eight synthesized domain
peptide sequences and three consensus sequences obtained by phage displayed
random peptide library. Basement membranes of endometrium of pregnant mice
and humans were immunostained with AK8 mAb. Thus, AK8 mAb recognized
a common structure present in the G domain of the laminin-1 chain in both
mice and humans. The passive immunization of mice with AK8 mAb may represent
a suitable animal model for anti-laminin-1 Ab-mediated reproductive failure. |
| format | Article |
| id | doaj-art-34fc99db475a4978ac5a28ec2404ddfe |
| institution | OA Journals |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-34fc99db475a4978ac5a28ec2404ddfe2025-08-20T02:24:05ZengWileyClinical and Developmental Immunology1740-25221740-25302005-01-01121677310.1080/17402520400014168Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1Akane Kondo0Junko Inagaki1Kazuko Kobayashi2Hideo Tsukamoto3Daisuke Yamamoto4Mikiya Nakatsuka5Nobuharu Suzuki6Motoyoshi Nomizu7Satoshi Amano8Hidehiko Matsubayashi9Tatsuji Yasuda10Luis R. Lopez11Yehuda Shoenfeld12Tsunehisa Makino13Eiji Matsuura14Department of Obstetrics and Gynecology, Center for Specialized Clinical Science, Tokai University, School of Medicine, Ishehara, JapanDepartment of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry, Shikata-cho, 2-5-1 Shikata-cho, Okayama 700-8558, JapanDepartment of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry, Shikata-cho, 2-5-1 Shikata-cho, Okayama 700-8558, JapanDepartment of Laboratory for Molecular Science Research, Tokai University, School of Medicine, Ishehara, JapanBiomedical Computation Center, Osaka Medical College, Takatsuki, JapanDepartment of Obstetrics and Gynecology, Okayama University Hospital, Okayama, JapanTokyo University of Pharmacy and Life Science, Hachiouji, JapanTokyo University of Pharmacy and Life Science, Hachiouji, JapanShiseido Life Science Research Center, Yokohama, JapanDepartment of Obstetrics and Gynecology, Center for Specialized Clinical Science, Tokai University, School of Medicine, Ishehara, JapanDepartment of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry, Shikata-cho, 2-5-1 Shikata-cho, Okayama 700-8558, JapanCorgenix Inc, Westminster, Colorado, USADepartment of Medicine B and Center for Autoimmune Diseases, Sheba Medical Center, Tel-hashomer, IsraelDepartment of Obstetrics and Gynecology, Center for Specialized Clinical Science, Tokai University, School of Medicine, Ishehara, JapanDepartment of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry, Shikata-cho, 2-5-1 Shikata-cho, Okayama 700-8558, JapanLaminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a significant association between anti-laminin-1 antibodies (Abs) and reproductive failure, such as recurrent spontaneous abortions and infertility-associated endometriosis in both human and mouse studies. In the present study, we established an IgM (μ,κ) monoclonal anti-laminin-1 Ab (AK8) by immunizing mice with mouse Engelbreth-Holm-Swarm sarcoma (EHS)-derived laminin-α1. The AK8 monoclonal antibody (mAb) reacted with particular peptide sequences from the globular G domain of mouse laminin-α1 chain of using ELISA and Western blot techniques. The peptide tertiary structure of the epitope recognized by AK8 mAb was predicted using eight synthesized domain peptide sequences and three consensus sequences obtained by phage displayed random peptide library. Basement membranes of endometrium of pregnant mice and humans were immunostained with AK8 mAb. Thus, AK8 mAb recognized a common structure present in the G domain of the laminin-1 chain in both mice and humans. The passive immunization of mice with AK8 mAb may represent a suitable animal model for anti-laminin-1 Ab-mediated reproductive failure.http://dx.doi.org/10.1080/17402520400014168 |
| spellingShingle | Akane Kondo Junko Inagaki Kazuko Kobayashi Hideo Tsukamoto Daisuke Yamamoto Mikiya Nakatsuka Nobuharu Suzuki Motoyoshi Nomizu Satoshi Amano Hidehiko Matsubayashi Tatsuji Yasuda Luis R. Lopez Yehuda Shoenfeld Tsunehisa Makino Eiji Matsuura Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1 Clinical and Developmental Immunology |
| title | Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1 |
| title_full | Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1 |
| title_fullStr | Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1 |
| title_full_unstemmed | Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1 |
| title_short | Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1 |
| title_sort | characterization of a murine anti laminin 1 monoclonal antibody ak8 produced by immunization with mouse derived laminin 1 |
| url | http://dx.doi.org/10.1080/17402520400014168 |
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