Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1
Laminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a significant association between anti-laminin-1 antibodies (Abs...
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| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2005-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520400014168 |
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| Summary: | Laminin-1 is a structural glycoprotein that forms an integral part of
the scaffolding of basement membranes, and plays an important role during
embryonic development. We have recently demonstrated a significant association
between anti-laminin-1 antibodies (Abs) and reproductive failure, such as recurrent
spontaneous abortions and infertility-associated endometriosis in both human and
mouse studies. In the present study, we established an IgM (μ,κ) monoclonal
anti-laminin-1 Ab (AK8) by immunizing mice with mouse Engelbreth-Holm-Swarm
sarcoma (EHS)-derived laminin-α1. The AK8 monoclonal antibody (mAb) reacted with
particular peptide sequences from the globular G domain of mouse laminin-α1 chain
of using ELISA and Western blot techniques. The peptide tertiary structure of
the epitope recognized by AK8 mAb was predicted using eight synthesized domain
peptide sequences and three consensus sequences obtained by phage displayed
random peptide library. Basement membranes of endometrium of pregnant mice
and humans were immunostained with AK8 mAb. Thus, AK8 mAb recognized
a common structure present in the G domain of the laminin-1 chain in both
mice and humans. The passive immunization of mice with AK8 mAb may represent
a suitable animal model for anti-laminin-1 Ab-mediated reproductive failure. |
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| ISSN: | 1740-2522 1740-2530 |