Evaluation of the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growth
Background and Objectives: Plant growth-promoting rhizobacteria (PGPR) with a diverse set of traits can improve crop yield in agriculture. The current study aimed to evaluate the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growth. Materials and Methods: In this stud...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2024-12-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4614 |
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| author | Ebrahim Eshaghi Sara Mousae Ali Hendiyani Alireza Habibi Khave Rahim Nosrati |
| author_facet | Ebrahim Eshaghi Sara Mousae Ali Hendiyani Alireza Habibi Khave Rahim Nosrati |
| author_sort | Ebrahim Eshaghi |
| collection | DOAJ |
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Background and Objectives: Plant growth-promoting rhizobacteria (PGPR) with a diverse set of traits can improve crop yield in agriculture. The current study aimed to evaluate the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growth.
Materials and Methods: In this study, 23 bacterial isolates were initially screened from maize plant rhizosphere. Ten isolates (A1–A10) were selected based on N fixation, P and K solubilization and their in vitro specific PGPR traits, such as solubilization of Zn, and Mn, the production of IAA, siderophore, ammonia, and HCN were assayed. Finally, the potential of selected isolates in enhancing the germination, height, shoot collar diameter, shoot fresh and dry weight biomass, and root dry weight of maize were evaluated.
Results: Among the positive-PGPR colonies, the selected isolates demonstrated the better performance of PGPR traits such as highest nitrogen fixation, P, K, Mn, and Zn solubilization, and production of siderophore, HCN, NH3, and IAA. In addition, the maize seed germination and improvement of maize yield in a pot experiment were observed after their treatment by bacterial inoculants. Biochemical characteristics, 16S rDNA amplification, and sequencing demonstrated a high similarity of PGPR isolates to the strains of Enterobacter, Pantoea, Kluyvera, Lelliottia, Klebsiella, Pectobacterium, and Stenotrophomonas.
Conclusion: The findings demonstrated that these strains could prove effective PGPR inoculants for the improvement of maize crops as multiple bio-fertilizers.
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| format | Article |
| id | doaj-art-34f5b1429abc4d228df9b6d7caade331 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-34f5b1429abc4d228df9b6d7caade3312025-01-06T08:38:43ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472024-12-0116610.18502/ijm.v16i6.17260Evaluation of the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growthEbrahim Eshaghi0Sara Mousae1Ali Hendiyani2Alireza Habibi Khave3Rahim Nosrati4Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, IranDepartment of Biology, Nourdanesh Institute of Higher Education, Meymeh, IranDepartment of Textile Engineering, Islamic Azad University, Science and Research Branch, Tehran, IranDepartment of Organic Chemistry, Payame Noor University, Tehran, IranCellular and Molecular Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran Background and Objectives: Plant growth-promoting rhizobacteria (PGPR) with a diverse set of traits can improve crop yield in agriculture. The current study aimed to evaluate the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growth. Materials and Methods: In this study, 23 bacterial isolates were initially screened from maize plant rhizosphere. Ten isolates (A1–A10) were selected based on N fixation, P and K solubilization and their in vitro specific PGPR traits, such as solubilization of Zn, and Mn, the production of IAA, siderophore, ammonia, and HCN were assayed. Finally, the potential of selected isolates in enhancing the germination, height, shoot collar diameter, shoot fresh and dry weight biomass, and root dry weight of maize were evaluated. Results: Among the positive-PGPR colonies, the selected isolates demonstrated the better performance of PGPR traits such as highest nitrogen fixation, P, K, Mn, and Zn solubilization, and production of siderophore, HCN, NH3, and IAA. In addition, the maize seed germination and improvement of maize yield in a pot experiment were observed after their treatment by bacterial inoculants. Biochemical characteristics, 16S rDNA amplification, and sequencing demonstrated a high similarity of PGPR isolates to the strains of Enterobacter, Pantoea, Kluyvera, Lelliottia, Klebsiella, Pectobacterium, and Stenotrophomonas. Conclusion: The findings demonstrated that these strains could prove effective PGPR inoculants for the improvement of maize crops as multiple bio-fertilizers. https://ijm.tums.ac.ir/index.php/ijm/article/view/4614Bio-fertilizer;Plant growth regulators;Rhizobacteria;Siderophore;Zea mays |
| spellingShingle | Ebrahim Eshaghi Sara Mousae Ali Hendiyani Alireza Habibi Khave Rahim Nosrati Evaluation of the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growth Iranian Journal of Microbiology Bio-fertilizer; Plant growth regulators; Rhizobacteria; Siderophore; Zea mays |
| title | Evaluation of the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growth |
| title_full | Evaluation of the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growth |
| title_fullStr | Evaluation of the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growth |
| title_full_unstemmed | Evaluation of the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growth |
| title_short | Evaluation of the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growth |
| title_sort | evaluation of the potential of multi trait pgpr isolates as inoculants for maize zea mays l growth |
| topic | Bio-fertilizer; Plant growth regulators; Rhizobacteria; Siderophore; Zea mays |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4614 |
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