miR-210 locus deletion disrupts cellular homeostasis: an integrated genetic study
Abstract MiR-210 is widely recognized as the quintessential hypoxia-responsive miRNA and is thought to fine-tune various facets of cellular homeostasis. We hereby present an integrative appraisal of the phenotypic and molecular repercussions of disrupting the corresponding locus in human and mouse c...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-07572-8 |
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| author | Mihai Bogdan Preda Evelyn Gabriela Nastase-Rusu Carmen Alexandra Neculachi Xiaoling Zhong Christine Voellenkle Nathalie M. Mazure Ovidiu Balacescu Cristina Ivan Xiao-Wei Zheng Mihaela Gherghiceanu Kevin Lebrigand Maya Simionescu Fabio Martelli Bernard Mari Sergiu-Bogdan Catrina Alexandrina Burlacu Mircea Ivan |
| author_facet | Mihai Bogdan Preda Evelyn Gabriela Nastase-Rusu Carmen Alexandra Neculachi Xiaoling Zhong Christine Voellenkle Nathalie M. Mazure Ovidiu Balacescu Cristina Ivan Xiao-Wei Zheng Mihaela Gherghiceanu Kevin Lebrigand Maya Simionescu Fabio Martelli Bernard Mari Sergiu-Bogdan Catrina Alexandrina Burlacu Mircea Ivan |
| author_sort | Mihai Bogdan Preda |
| collection | DOAJ |
| description | Abstract MiR-210 is widely recognized as the quintessential hypoxia-responsive miRNA and is thought to fine-tune various facets of cellular homeostasis. We hereby present an integrative appraisal of the phenotypic and molecular repercussions of disrupting the corresponding locus in human and mouse cells using multiple genetic strategies. In brief, MIR210 deletion led to decreased cellular fitness and suboptimal responses to several stress types. Transcriptomic comparisons via different profiling platforms, performed independently by members of this collaboration, revealed consistent deregulation of neighboring genes, in locus-disrupted cells. Interestingly, the anticipated enrichment of miR-210 targets failed to materialize in unbiased analyses. Our results point to the biological significance of unrecognized regulatory elements that overlap miRNA genes and should serve as a note of caution for studies based on the genetic disruption of such loci. |
| format | Article |
| id | doaj-art-34f55d28c44d4e05be4f00bc637f0850 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-34f55d28c44d4e05be4f00bc637f08502025-08-20T03:37:24ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-07572-8miR-210 locus deletion disrupts cellular homeostasis: an integrated genetic studyMihai Bogdan Preda0Evelyn Gabriela Nastase-Rusu1Carmen Alexandra Neculachi2Xiaoling Zhong3Christine Voellenkle4Nathalie M. Mazure5Ovidiu Balacescu6Cristina Ivan7Xiao-Wei Zheng8Mihaela Gherghiceanu9Kevin Lebrigand10Maya Simionescu11Fabio Martelli12Bernard Mari13Sergiu-Bogdan Catrina14Alexandrina Burlacu15Mircea Ivan16Department of Stem Cell Biology, Institute of Cellular Biology and Pathology “Nicolae Simionescu”Department of Stem Cell Biology, Institute of Cellular Biology and Pathology “Nicolae Simionescu”Department of Stem Cell Biology, Institute of Cellular Biology and Pathology “Nicolae Simionescu”Department of Medicine, Indiana University School of MedicineDepartment of Stem Cell Biology, Institute of Cellular Biology and Pathology “Nicolae Simionescu”INSERM U1065, C3M, Université Côte d’AzurDepartment of Genetics, Genomics and Experimental Pathology, The Oncology Institute “Prof. Dr. Ion Chiricuta”Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer CenterDepartment of Molecular Medicine and Surgery, Karolinska InstitutetVictor Babeș National Institute of PathologyCNRS UMR7275 Inserm 1323, IPMC, FHU- OncoAge, IHU RespiERA, Sophia Antipolis, Université Côte d’AzurDepartment of Stem Cell Biology, Institute of Cellular Biology and Pathology “Nicolae Simionescu”Department of Stem Cell Biology, Institute of Cellular Biology and Pathology “Nicolae Simionescu”CNRS UMR7275 Inserm 1323, IPMC, FHU- OncoAge, IHU RespiERA, Sophia Antipolis, Université Côte d’AzurDepartment of Molecular Medicine and Surgery, Karolinska InstitutetDepartment of Stem Cell Biology, Institute of Cellular Biology and Pathology “Nicolae Simionescu”Department of Medicine, Indiana University School of MedicineAbstract MiR-210 is widely recognized as the quintessential hypoxia-responsive miRNA and is thought to fine-tune various facets of cellular homeostasis. We hereby present an integrative appraisal of the phenotypic and molecular repercussions of disrupting the corresponding locus in human and mouse cells using multiple genetic strategies. In brief, MIR210 deletion led to decreased cellular fitness and suboptimal responses to several stress types. Transcriptomic comparisons via different profiling platforms, performed independently by members of this collaboration, revealed consistent deregulation of neighboring genes, in locus-disrupted cells. Interestingly, the anticipated enrichment of miR-210 targets failed to materialize in unbiased analyses. Our results point to the biological significance of unrecognized regulatory elements that overlap miRNA genes and should serve as a note of caution for studies based on the genetic disruption of such loci.https://doi.org/10.1038/s41598-025-07572-8Gene editing technologiesGenomic neighborhoodHypoxiamiR-210Targets |
| spellingShingle | Mihai Bogdan Preda Evelyn Gabriela Nastase-Rusu Carmen Alexandra Neculachi Xiaoling Zhong Christine Voellenkle Nathalie M. Mazure Ovidiu Balacescu Cristina Ivan Xiao-Wei Zheng Mihaela Gherghiceanu Kevin Lebrigand Maya Simionescu Fabio Martelli Bernard Mari Sergiu-Bogdan Catrina Alexandrina Burlacu Mircea Ivan miR-210 locus deletion disrupts cellular homeostasis: an integrated genetic study Scientific Reports Gene editing technologies Genomic neighborhood Hypoxia miR-210 Targets |
| title | miR-210 locus deletion disrupts cellular homeostasis: an integrated genetic study |
| title_full | miR-210 locus deletion disrupts cellular homeostasis: an integrated genetic study |
| title_fullStr | miR-210 locus deletion disrupts cellular homeostasis: an integrated genetic study |
| title_full_unstemmed | miR-210 locus deletion disrupts cellular homeostasis: an integrated genetic study |
| title_short | miR-210 locus deletion disrupts cellular homeostasis: an integrated genetic study |
| title_sort | mir 210 locus deletion disrupts cellular homeostasis an integrated genetic study |
| topic | Gene editing technologies Genomic neighborhood Hypoxia miR-210 Targets |
| url | https://doi.org/10.1038/s41598-025-07572-8 |
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