Preparation of Pinoresinol and Dehydrodiconiferyl Alcohol from Eucommiae Cortex Extract by Fermentation with Traditional Mucor
Eucommiae Cortex (EC) is frequently used alone or in combination with other active ingredients to treat a range of illnesses. An efficient technical instrument for changing cheap or plentiful organic chemicals into rare or costly counterparts is biotransformation. It combines EC with biotransformati...
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MDPI AG
2024-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/13/2979 |
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| author | Wenyi Jiang Zhengyou He Ruijiao Yao Zhiyang Chen Xia Zeng Miao Zheng Jing Wang Jia Li Yong Jiang |
| author_facet | Wenyi Jiang Zhengyou He Ruijiao Yao Zhiyang Chen Xia Zeng Miao Zheng Jing Wang Jia Li Yong Jiang |
| author_sort | Wenyi Jiang |
| collection | DOAJ |
| description | Eucommiae Cortex (EC) is frequently used alone or in combination with other active ingredients to treat a range of illnesses. An efficient technical instrument for changing cheap or plentiful organic chemicals into rare or costly counterparts is biotransformation. It combines EC with biotransformation techniques with the aim of producing some novel active ingredients, using different strains of bacteria that were introduced to biotransform EC in an aseptic environment. The high-quality strains were screened for identification after the fermentation broth was found using HPLC, and the primary unidentified chemicals were separated and purified in order to be structurally identified. Strain 1 was identified as <i>Aspergillus niger</i> and strain 2 as <i>Actinomucor elegans</i>; the main transformation product A was identified as pinoresinol (Pin) and B as dehydrodiconiferyl alcohol (DA). The biotransformation of EC utilizing <i>Aspergillus niger</i> and <i>Actinomucor elegans</i> is reported for the first time in this study’s conclusion, resulting in the production of Pin and DA. |
| format | Article |
| id | doaj-art-34f4d701eb8b458698776c221069268f |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-34f4d701eb8b458698776c221069268f2025-08-20T02:57:34ZengMDPI AGMolecules1420-30492024-06-012913297910.3390/molecules29132979Preparation of Pinoresinol and Dehydrodiconiferyl Alcohol from Eucommiae Cortex Extract by Fermentation with Traditional MucorWenyi Jiang0Zhengyou He1Ruijiao Yao2Zhiyang Chen3Xia Zeng4Miao Zheng5Jing Wang6Jia Li7Yong Jiang8School of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaSchool of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaSchool of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaSchool of Pharmacy, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610106, ChinaSchool of Pharmacy, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610106, ChinaSchool of Pharmacy, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610106, ChinaSchool of Pharmacy, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610106, ChinaSchool of Pharmacy, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610106, ChinaSchool of Pharmacy, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610106, ChinaEucommiae Cortex (EC) is frequently used alone or in combination with other active ingredients to treat a range of illnesses. An efficient technical instrument for changing cheap or plentiful organic chemicals into rare or costly counterparts is biotransformation. It combines EC with biotransformation techniques with the aim of producing some novel active ingredients, using different strains of bacteria that were introduced to biotransform EC in an aseptic environment. The high-quality strains were screened for identification after the fermentation broth was found using HPLC, and the primary unidentified chemicals were separated and purified in order to be structurally identified. Strain 1 was identified as <i>Aspergillus niger</i> and strain 2 as <i>Actinomucor elegans</i>; the main transformation product A was identified as pinoresinol (Pin) and B as dehydrodiconiferyl alcohol (DA). The biotransformation of EC utilizing <i>Aspergillus niger</i> and <i>Actinomucor elegans</i> is reported for the first time in this study’s conclusion, resulting in the production of Pin and DA.https://www.mdpi.com/1420-3049/29/13/2979Eucommiae Cortexbiotransformation<i>Aspergillus niger</i><i>Actinomucor elegans</i>pinoresinoldehydrodiconiferyl alcohol |
| spellingShingle | Wenyi Jiang Zhengyou He Ruijiao Yao Zhiyang Chen Xia Zeng Miao Zheng Jing Wang Jia Li Yong Jiang Preparation of Pinoresinol and Dehydrodiconiferyl Alcohol from Eucommiae Cortex Extract by Fermentation with Traditional Mucor Molecules Eucommiae Cortex biotransformation <i>Aspergillus niger</i> <i>Actinomucor elegans</i> pinoresinol dehydrodiconiferyl alcohol |
| title | Preparation of Pinoresinol and Dehydrodiconiferyl Alcohol from Eucommiae Cortex Extract by Fermentation with Traditional Mucor |
| title_full | Preparation of Pinoresinol and Dehydrodiconiferyl Alcohol from Eucommiae Cortex Extract by Fermentation with Traditional Mucor |
| title_fullStr | Preparation of Pinoresinol and Dehydrodiconiferyl Alcohol from Eucommiae Cortex Extract by Fermentation with Traditional Mucor |
| title_full_unstemmed | Preparation of Pinoresinol and Dehydrodiconiferyl Alcohol from Eucommiae Cortex Extract by Fermentation with Traditional Mucor |
| title_short | Preparation of Pinoresinol and Dehydrodiconiferyl Alcohol from Eucommiae Cortex Extract by Fermentation with Traditional Mucor |
| title_sort | preparation of pinoresinol and dehydrodiconiferyl alcohol from eucommiae cortex extract by fermentation with traditional mucor |
| topic | Eucommiae Cortex biotransformation <i>Aspergillus niger</i> <i>Actinomucor elegans</i> pinoresinol dehydrodiconiferyl alcohol |
| url | https://www.mdpi.com/1420-3049/29/13/2979 |
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