Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions
Mycobacterium avium subsp paratuberculosis (Map), the causative agent of Johne's disease, has a robust ability to survive in the environment. However, the ability of Map to migrate through soil to drainage tiles or ground water, leave the farm, and leak into local watersheds is inadequately doc...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2011/506239 |
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| author | Eran A. Raizman Mussie Y. Habteselassie Ching C. Wu Tsang L. Lin M. Negron Ronald F. Turco |
| author_facet | Eran A. Raizman Mussie Y. Habteselassie Ching C. Wu Tsang L. Lin M. Negron Ronald F. Turco |
| author_sort | Eran A. Raizman |
| collection | DOAJ |
| description | Mycobacterium avium subsp paratuberculosis (Map), the causative agent of Johne's disease, has a robust ability to survive in the environment. However, the ability of Map to migrate through soil to drainage tiles or ground water, leave the farm, and leak into local watersheds is inadequately documented. In order to assess the ability of Map to leach through soil, two laboratory experiments were conducted. In the first study, 8 columns (30 cm long each) of a sandy loam soil were treated with pure cultures of Map. Two soil moisture levels and two Map concentrations were used. The columns were leached with 500 mL of water once a week for three weeks, the leachate was collected, and detection analysis was conducted. In the second experiment, manure from Map negative cows (control) and Map high shedder cows (treatment) were deposited on 8 similar columns and the columns were leached with 500 mL of water once a week for four weeks. Map detection and numeration in leachate samples were done with RT-PCR and culture techniques, respectively. Using RT-PCR, Map could be detected in the leachates in both experiments for several weeks but could only be recovered using culture techniques in experiment one. Combined, these experiments indicate the potential for Map to move through soil as a result of rainfall or irrigation following application. |
| format | Article |
| id | doaj-art-34f0ab9883fc4f40b075e224bee60293 |
| institution | OA Journals |
| issn | 2042-0048 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-34f0ab9883fc4f40b075e224bee602932025-08-20T02:24:05ZengWileyVeterinary Medicine International2042-00482011-01-01201110.4061/2011/506239506239Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro ConditionsEran A. Raizman0Mussie Y. Habteselassie1Ching C. Wu2Tsang L. Lin3M. Negron4Ronald F. Turco5Department of Comparative Pathobiology School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USADepartment of Crop and Soil Sciences, University of Georgia, Griffin, GA 30223, USADepartment of Comparative Pathobiology School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USADepartment of Comparative Pathobiology School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USADepartment of Comparative Pathobiology School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USAAgronomy Department, College of Agriculture, Purdue University, West Lafayette, IN 47907, USAMycobacterium avium subsp paratuberculosis (Map), the causative agent of Johne's disease, has a robust ability to survive in the environment. However, the ability of Map to migrate through soil to drainage tiles or ground water, leave the farm, and leak into local watersheds is inadequately documented. In order to assess the ability of Map to leach through soil, two laboratory experiments were conducted. In the first study, 8 columns (30 cm long each) of a sandy loam soil were treated with pure cultures of Map. Two soil moisture levels and two Map concentrations were used. The columns were leached with 500 mL of water once a week for three weeks, the leachate was collected, and detection analysis was conducted. In the second experiment, manure from Map negative cows (control) and Map high shedder cows (treatment) were deposited on 8 similar columns and the columns were leached with 500 mL of water once a week for four weeks. Map detection and numeration in leachate samples were done with RT-PCR and culture techniques, respectively. Using RT-PCR, Map could be detected in the leachates in both experiments for several weeks but could only be recovered using culture techniques in experiment one. Combined, these experiments indicate the potential for Map to move through soil as a result of rainfall or irrigation following application.http://dx.doi.org/10.4061/2011/506239 |
| spellingShingle | Eran A. Raizman Mussie Y. Habteselassie Ching C. Wu Tsang L. Lin M. Negron Ronald F. Turco Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions Veterinary Medicine International |
| title | Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions |
| title_full | Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions |
| title_fullStr | Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions |
| title_full_unstemmed | Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions |
| title_short | Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions |
| title_sort | leaching of mycobacterium avium subsp paratuberculosis in soil under in vitro conditions |
| url | http://dx.doi.org/10.4061/2011/506239 |
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