Investigation of persistant bovine viral diarrhea virus (bvdv) infection at the pregnant cows and their calves bv immunoflourescence and immunperoxidase tests

Blood samples which were collected with and without EDTA were taken from 62 prenatal pregnant cows, from both cows and precolostral calves at the time of birth and, after approximately 3 months, from postnatal calves which were all present in a private management in this research. Direct immunoflour...

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Main Authors: Orhan Yapkıç, Sibel Yavru
Format: Article
Language:English
Published: Selcuk University Press
Series:Eurasian Journal of Veterinary Sciences
Subjects:
Online Access:http://eurasianjvetsci.org/pdf.php3?id=294
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author Orhan Yapkıç
Sibel Yavru
author_facet Orhan Yapkıç
Sibel Yavru
author_sort Orhan Yapkıç
collection DOAJ
description Blood samples which were collected with and without EDTA were taken from 62 prenatal pregnant cows, from both cows and precolostral calves at the time of birth and, after approximately 3 months, from postnatal calves which were all present in a private management in this research. Direct immunoflourescence (DIF) and direct immunperoxidase (DPLA) tests were used for virus isolation from leucocyte samples, serum neutralisation test was used to detect antibodies against BVDV. Viral antigen wasn't delected in BVDV from first and second blood samples obtained both all pregnant cows and all calves. By SN testing which was used to detect antibodies against BVDV. while all the pregnant cows were found to be positive after both sampling, 7 precolostral calves were positive and 55 precolostral calves were negative all the calves, from which the second sampling was carried out approximately during 3 months after birth, were detected as positive. SN<sub>50</sub> titers of the pregnant cows from which sera samples were collected before birth were noted between 1/7,5 and 1/160&#8804; However, SN<sub>50</sub> titers of sera taken from cows just after giving birth were detected between 1/7,5 and 1/160&#8804; The sera samplss obtained from 7 precolostral - seropositive calves immediately following birth, SN<sub>50</sub> titers were lound to be between 1/20 and1/120. The samples taken from calves within 3 months after birth SN<sub>50</sub> tilers were detected between1/5 and 1/160.
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institution Kabale University
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record_format Article
series Eurasian Journal of Veterinary Sciences
spelling doaj-art-34eed1ec81084aabad03a29c65ef1c922025-02-03T11:25:44ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19531732130294Investigation of persistant bovine viral diarrhea virus (bvdv) infection at the pregnant cows and their calves bv immunoflourescence and immunperoxidase testsOrhan YapkıçSibel YavruBlood samples which were collected with and without EDTA were taken from 62 prenatal pregnant cows, from both cows and precolostral calves at the time of birth and, after approximately 3 months, from postnatal calves which were all present in a private management in this research. Direct immunoflourescence (DIF) and direct immunperoxidase (DPLA) tests were used for virus isolation from leucocyte samples, serum neutralisation test was used to detect antibodies against BVDV. Viral antigen wasn't delected in BVDV from first and second blood samples obtained both all pregnant cows and all calves. By SN testing which was used to detect antibodies against BVDV. while all the pregnant cows were found to be positive after both sampling, 7 precolostral calves were positive and 55 precolostral calves were negative all the calves, from which the second sampling was carried out approximately during 3 months after birth, were detected as positive. SN<sub>50</sub> titers of the pregnant cows from which sera samples were collected before birth were noted between 1/7,5 and 1/160&#8804; However, SN<sub>50</sub> titers of sera taken from cows just after giving birth were detected between 1/7,5 and 1/160&#8804; The sera samplss obtained from 7 precolostral - seropositive calves immediately following birth, SN<sub>50</sub> titers were lound to be between 1/20 and1/120. The samples taken from calves within 3 months after birth SN<sub>50</sub> tilers were detected between1/5 and 1/160.http://eurasianjvetsci.org/pdf.php3?id=294bvdvpregnant cattlepersistent infectioncalves. difdplasn
spellingShingle Orhan Yapkıç
Sibel Yavru
Investigation of persistant bovine viral diarrhea virus (bvdv) infection at the pregnant cows and their calves bv immunoflourescence and immunperoxidase tests
Eurasian Journal of Veterinary Sciences
bvdv
pregnant cattle
persistent infection
calves. dif
dpla
sn
title Investigation of persistant bovine viral diarrhea virus (bvdv) infection at the pregnant cows and their calves bv immunoflourescence and immunperoxidase tests
title_full Investigation of persistant bovine viral diarrhea virus (bvdv) infection at the pregnant cows and their calves bv immunoflourescence and immunperoxidase tests
title_fullStr Investigation of persistant bovine viral diarrhea virus (bvdv) infection at the pregnant cows and their calves bv immunoflourescence and immunperoxidase tests
title_full_unstemmed Investigation of persistant bovine viral diarrhea virus (bvdv) infection at the pregnant cows and their calves bv immunoflourescence and immunperoxidase tests
title_short Investigation of persistant bovine viral diarrhea virus (bvdv) infection at the pregnant cows and their calves bv immunoflourescence and immunperoxidase tests
title_sort investigation of persistant bovine viral diarrhea virus bvdv infection at the pregnant cows and their calves bv immunoflourescence and immunperoxidase tests
topic bvdv
pregnant cattle
persistent infection
calves. dif
dpla
sn
url http://eurasianjvetsci.org/pdf.php3?id=294
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AT sibelyavru investigationofpersistantbovineviraldiarrheavirusbvdvinfectionatthepregnantcowsandtheircalvesbvimmunoflourescenceandimmunperoxidasetests