Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor
Highlights A collaborative biosensing system is developed for amplification-free RNA/miRNA detection by integrating Type III CRISPR-Cas10 system with a graphene field-effect transistor (GFET). Continuous cleavage of ssDNA by the mutant CRISPR-Cas10 effector complexes and high charge density of hairp...
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-04-01
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| Series: | Nano-Micro Letters |
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| Online Access: | https://doi.org/10.1007/s40820-025-01730-3 |
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| _version_ | 1849761384165801984 |
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| author | Mingyuan Sun Zhenxiao Yu Shuai Wang Jiaoyan Qiu Yuzhen Huang Xiaoshuang Chen Yunhong Zhang Chao Wang Xue Zhang Yanbo Liang Hong Liu Qunxin She Yu Zhang Lin Han |
| author_facet | Mingyuan Sun Zhenxiao Yu Shuai Wang Jiaoyan Qiu Yuzhen Huang Xiaoshuang Chen Yunhong Zhang Chao Wang Xue Zhang Yanbo Liang Hong Liu Qunxin She Yu Zhang Lin Han |
| author_sort | Mingyuan Sun |
| collection | DOAJ |
| description | Highlights A collaborative biosensing system is developed for amplification-free RNA/miRNA detection by integrating Type III CRISPR-Cas10 system with a graphene field-effect transistor (GFET). Continuous cleavage of ssDNA by the mutant CRISPR-Cas10 effector complexes and high charge density of hairpin DNA reporters on the GFET channel enable the detection limit to reach the level of aM. A universal sensing detection platform is established to directly detect the medium-length RNAs and miRNAs in clinical samples with the recognition capability of single nucleic acid. |
| format | Article |
| id | doaj-art-34dfa186efe54dac8edef5d6a5451afa |
| institution | DOAJ |
| issn | 2311-6706 2150-5551 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nano-Micro Letters |
| spelling | doaj-art-34dfa186efe54dac8edef5d6a5451afa2025-08-20T03:06:02ZengSpringerOpenNano-Micro Letters2311-67062150-55512025-04-0117111910.1007/s40820-025-01730-3Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect TransistorMingyuan Sun0Zhenxiao Yu1Shuai Wang2Jiaoyan Qiu3Yuzhen Huang4Xiaoshuang Chen5Yunhong Zhang6Chao Wang7Xue Zhang8Yanbo Liang9Hong Liu10Qunxin She11Yu Zhang12Lin Han13Institute of Marine Science and Technology, Shandong UniversityCRISPR and Archaea Biology Research Center, State Key Laboratory of Microbial Technology and Microbial Technology Institute, Shandong UniversityInstitute of Marine Science and Technology, Shandong UniversityInstitute of Marine Science and Technology, Shandong UniversityInstitute of Marine Science and Technology, Shandong UniversityInstitute of Marine Science and Technology, Shandong UniversityInstitute of Marine Science and Technology, Shandong UniversityInstitute of Marine Science and Technology, Shandong UniversityInstitute of Marine Science and Technology, Shandong UniversityInstitute of Marine Science and Technology, Shandong UniversityState Key Laboratory of Crystal Materials, Shandong UniversityCRISPR and Archaea Biology Research Center, State Key Laboratory of Microbial Technology and Microbial Technology Institute, Shandong UniversityInstitute of Marine Science and Technology, Shandong UniversityInstitute of Marine Science and Technology, Shandong UniversityHighlights A collaborative biosensing system is developed for amplification-free RNA/miRNA detection by integrating Type III CRISPR-Cas10 system with a graphene field-effect transistor (GFET). Continuous cleavage of ssDNA by the mutant CRISPR-Cas10 effector complexes and high charge density of hairpin DNA reporters on the GFET channel enable the detection limit to reach the level of aM. A universal sensing detection platform is established to directly detect the medium-length RNAs and miRNAs in clinical samples with the recognition capability of single nucleic acid.https://doi.org/10.1007/s40820-025-01730-3CRISPRCas10; Graphene effectField transistor; Biosensor; RNA/miRNA detection; AmplificationFree |
| spellingShingle | Mingyuan Sun Zhenxiao Yu Shuai Wang Jiaoyan Qiu Yuzhen Huang Xiaoshuang Chen Yunhong Zhang Chao Wang Xue Zhang Yanbo Liang Hong Liu Qunxin She Yu Zhang Lin Han Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor Nano-Micro Letters CRISPR Cas10; Graphene effect Field transistor; Biosensor; RNA/miRNA detection; Amplification Free |
| title | Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor |
| title_full | Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor |
| title_fullStr | Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor |
| title_full_unstemmed | Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor |
| title_short | Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor |
| title_sort | universal amplification free rna detection by integrating crispr cas10 with aptameric graphene field effect transistor |
| topic | CRISPR Cas10; Graphene effect Field transistor; Biosensor; RNA/miRNA detection; Amplification Free |
| url | https://doi.org/10.1007/s40820-025-01730-3 |
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