Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor

Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor

Highlights A collaborative biosensing system is developed for amplification-free RNA/miRNA detection by integrating Type III CRISPR-Cas10 system with a graphene field-effect transistor (GFET). Continuous cleavage of ssDNA by the mutant CRISPR-Cas10 effector complexes and high charge density of hairp...

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Main Authors: Mingyuan Sun, Zhenxiao Yu, Shuai Wang, Jiaoyan Qiu, Yuzhen Huang, Xiaoshuang Chen, Yunhong Zhang, Chao Wang, Xue Zhang, Yanbo Liang, Hong Liu, Qunxin She, Yu Zhang, Lin Han
Format: Article
Language:English
Published: SpringerOpen 2025-04-01
Series:Nano-Micro Letters
Subjects:
CRISPR
Cas10; Graphene effect
Field transistor; Biosensor; RNA/miRNA detection; Amplification
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Online Access:https://doi.org/10.1007/s40820-025-01730-3
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Summary:Highlights A collaborative biosensing system is developed for amplification-free RNA/miRNA detection by integrating Type III CRISPR-Cas10 system with a graphene field-effect transistor (GFET). Continuous cleavage of ssDNA by the mutant CRISPR-Cas10 effector complexes and high charge density of hairpin DNA reporters on the GFET channel enable the detection limit to reach the level of aM. A universal sensing detection platform is established to directly detect the medium-length RNAs and miRNAs in clinical samples with the recognition capability of single nucleic acid.
ISSN:2311-6706
2150-5551

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