Indirect Effects of Oral Tolerance Inhibit Pulmonary Granulomas to Schistosoma mansoni Eggs

Parenteral injection of tolerated proteins into orally tolerant mice inhibits the initiation of immunological responses to unrelated proteins and blocks severe chronic inflammatory reactions of immunological origin, such as autoimmune reactions. This inhibitory effect which we have called “indirect...

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Main Authors: Geraldo Magela Azevedo, Raquel Alves Costa, Mariana Araujo Resende, Claudiney Melquiades Rodrigues, Nelson Monteiro Vaz, Cláudia Rocha Carvalho
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:Clinical and Developmental Immunology
Online Access:http://dx.doi.org/10.1155/2012/293625
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author Geraldo Magela Azevedo
Raquel Alves Costa
Mariana Araujo Resende
Claudiney Melquiades Rodrigues
Nelson Monteiro Vaz
Cláudia Rocha Carvalho
author_facet Geraldo Magela Azevedo
Raquel Alves Costa
Mariana Araujo Resende
Claudiney Melquiades Rodrigues
Nelson Monteiro Vaz
Cláudia Rocha Carvalho
author_sort Geraldo Magela Azevedo
collection DOAJ
description Parenteral injection of tolerated proteins into orally tolerant mice inhibits the initiation of immunological responses to unrelated proteins and blocks severe chronic inflammatory reactions of immunological origin, such as autoimmune reactions. This inhibitory effect which we have called “indirect effects of oral tolerance” is also known as “bystander suppression.” Herein, we show that i.p. injection of OVA + Al(OH)3 minutes before i.v. injection of Schistosoma mansoni eggs into OVA tolerant mice blocked the increase of pulmonary granulomas. In addition, the expression of ICAM-1 in lung parenchyma in areas outside the granulomas of OVA-orally tolerant mice was significantly reduced. However, at day 18 after granuloma induction there was no difference in immunofluorescency intensity to CD3, CD4, F4/80, andα-SMA per granuloma area of tolerant and control groups. Reduction of granulomas by reexposure to orally tolerated proteins was not correlated with a shift in Th-1/Th-2 cytokines in serum or lung tissue extract.
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language English
publishDate 2012-01-01
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series Clinical and Developmental Immunology
spelling doaj-art-34d32c78fa4644eb9cd7f88d2c26c8792025-08-20T02:24:04ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/293625293625Indirect Effects of Oral Tolerance Inhibit Pulmonary Granulomas to Schistosoma mansoni EggsGeraldo Magela Azevedo0Raquel Alves Costa1Mariana Araujo Resende2Claudiney Melquiades Rodrigues3Nelson Monteiro Vaz4Cláudia Rocha Carvalho5Departamento de Morfologia, ICB-UFMG, Avenue Antônio Carlos 6627, Pampulha, Belo Horizonte, MG, CEP 31270-901, BrazilDepartamento de Morfologia, ICB-UFMG, Avenue Antônio Carlos 6627, Pampulha, Belo Horizonte, MG, CEP 31270-901, BrazilDepartamento de Morfologia, ICB-UFMG, Avenue Antônio Carlos 6627, Pampulha, Belo Horizonte, MG, CEP 31270-901, BrazilDepartamento de Morfologia, ICB-UFMG, Avenue Antônio Carlos 6627, Pampulha, Belo Horizonte, MG, CEP 31270-901, BrazilDepartamentos de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, CEP 31270-901, BrazilDepartamento de Morfologia, ICB-UFMG, Avenue Antônio Carlos 6627, Pampulha, Belo Horizonte, MG, CEP 31270-901, BrazilParenteral injection of tolerated proteins into orally tolerant mice inhibits the initiation of immunological responses to unrelated proteins and blocks severe chronic inflammatory reactions of immunological origin, such as autoimmune reactions. This inhibitory effect which we have called “indirect effects of oral tolerance” is also known as “bystander suppression.” Herein, we show that i.p. injection of OVA + Al(OH)3 minutes before i.v. injection of Schistosoma mansoni eggs into OVA tolerant mice blocked the increase of pulmonary granulomas. In addition, the expression of ICAM-1 in lung parenchyma in areas outside the granulomas of OVA-orally tolerant mice was significantly reduced. However, at day 18 after granuloma induction there was no difference in immunofluorescency intensity to CD3, CD4, F4/80, andα-SMA per granuloma area of tolerant and control groups. Reduction of granulomas by reexposure to orally tolerated proteins was not correlated with a shift in Th-1/Th-2 cytokines in serum or lung tissue extract.http://dx.doi.org/10.1155/2012/293625
spellingShingle Geraldo Magela Azevedo
Raquel Alves Costa
Mariana Araujo Resende
Claudiney Melquiades Rodrigues
Nelson Monteiro Vaz
Cláudia Rocha Carvalho
Indirect Effects of Oral Tolerance Inhibit Pulmonary Granulomas to Schistosoma mansoni Eggs
Clinical and Developmental Immunology
title Indirect Effects of Oral Tolerance Inhibit Pulmonary Granulomas to Schistosoma mansoni Eggs
title_full Indirect Effects of Oral Tolerance Inhibit Pulmonary Granulomas to Schistosoma mansoni Eggs
title_fullStr Indirect Effects of Oral Tolerance Inhibit Pulmonary Granulomas to Schistosoma mansoni Eggs
title_full_unstemmed Indirect Effects of Oral Tolerance Inhibit Pulmonary Granulomas to Schistosoma mansoni Eggs
title_short Indirect Effects of Oral Tolerance Inhibit Pulmonary Granulomas to Schistosoma mansoni Eggs
title_sort indirect effects of oral tolerance inhibit pulmonary granulomas to schistosoma mansoni eggs
url http://dx.doi.org/10.1155/2012/293625
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