Chemical analysis using miniaturized and Portable 3D printed systems: Where are we now?

With the growing emphasis on green chemistry (GC) and green analytical chemistry (GAC), significant efforts have been directed toward minimizing sample size and simplifying sample preparation procedures. Concurrently, ongoing research has enabled the miniaturization of instrumental configurations us...

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Main Authors: Miryam Perrucci, Imran Ali, Fotouh R. Mansour, Halil I. Ulusoy, Songül Ulusoy, Abuzar Kabir, Ornella Abollino, Agnese Giacomino, Paolo Inaudi, Marcello Locatelli, Erika Maria Ricci
Format: Article
Language:English
Published: Elsevier 2025-11-01
Series:Journal of Chromatography Open
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772391725000398
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author Miryam Perrucci
Imran Ali
Fotouh R. Mansour
Halil I. Ulusoy
Songül Ulusoy
Abuzar Kabir
Ornella Abollino
Agnese Giacomino
Paolo Inaudi
Marcello Locatelli
Erika Maria Ricci
author_facet Miryam Perrucci
Imran Ali
Fotouh R. Mansour
Halil I. Ulusoy
Songül Ulusoy
Abuzar Kabir
Ornella Abollino
Agnese Giacomino
Paolo Inaudi
Marcello Locatelli
Erika Maria Ricci
author_sort Miryam Perrucci
collection DOAJ
description With the growing emphasis on green chemistry (GC) and green analytical chemistry (GAC), significant efforts have been directed toward minimizing sample size and simplifying sample preparation procedures. Concurrently, ongoing research has enabled the miniaturization of instrumental configurations used for quantitative analysis, aligning with the principles of sustainability.One major challenge lies in the inherent complexity of liquid chromatography systems, which comprise multiple interconnected components. Each component has been subject to miniaturization, with careful attention to preserving analytical sensitivity, selectivity, and accuracy when applied to real samples. Interest in miniaturized systems is further fueled by the theoretical principle that reducing flow rates enhances the surface-to-volume ratio, thereby improving detection sensitivity.Building on this theoretical foundation—particularly the goals of portability, efficient sampling, and on site analysis—innovative approaches such as microfluidics and 3D printing have been employed to develop compact fluidic systems. These technologies not only broaden the scope of liquid chromatography but also open new frontiers, such as drug testing on engineered tissues, and the development of new devices suitable for space missions.This review aims to present a comprehensive overview of the challenges encountered and the advancements achieved in the miniaturization of instrumental setups and portable systems, encompassing both pre- and post-analytical stages. Furthermore, a discussion on 3D technologies available for LC-chip and miniaturized systems was also presented.
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spelling doaj-art-34cc13cfea394e0a8ff3dbabb20a49862025-08-20T03:13:22ZengElsevierJournal of Chromatography Open2772-39172025-11-01810024110.1016/j.jcoa.2025.100241Chemical analysis using miniaturized and Portable 3D printed systems: Where are we now?Miryam Perrucci0Imran Ali1Fotouh R. Mansour2Halil I. Ulusoy3Songül Ulusoy4Abuzar Kabir5Ornella Abollino6Agnese Giacomino7Paolo Inaudi8Marcello Locatelli9Erika Maria Ricci10University of Teramo, Department of Biosciences and Agro-Food and Environmental Technologies, Teramo 64100, Italy; Department of Innovative Technologies in Medicine & Dentistry, University “G. d’Annunzio” of Chieti-Pescara, Chieti 66100, ItalyJamia Millia Islamia, Jamia Nagar, New Delhi 110025, IndiaThe medical campus of Tanta University, Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Elgeish Street, Tanta, EgyptDepartment of Analytical Chemistry, Faculty of Pharmacy, Cumhuriyet University, Sivas 58140, TurkeyDepartment of Pharmacy, Vocational School of Health Service, Sivas Cumhuriyet University, Sivas 58140, TurkeyDepartment of Chemistry and Biochemistry, International Forensic Research Institute, Florida International Univeristy, Miami, FL 33199, USADepartment of Drug Science and Technology, University of Torino, 10125 Torino, ItalyDepartment of Drug Science and Technology, University of Torino, 10125 Torino, ItalyDepartment of Drug Science and Technology, University of Torino, 10125 Torino, ItalyAnalytical and Bioanalytical Chemistry Laboratory - Department of Science - University “G. d’Annunzio” of Chieti-Pescara, Chieti 66100, Italy; Corresponding author at: Department of Science - University “G. d’Annunzio” Chieti-Pescara, Italy.Analytical and Bioanalytical Chemistry Laboratory - Department of Pharmacy - University “G. d’Annunzio” of Chieti-Pescara, Chieti 66100, ItalyWith the growing emphasis on green chemistry (GC) and green analytical chemistry (GAC), significant efforts have been directed toward minimizing sample size and simplifying sample preparation procedures. Concurrently, ongoing research has enabled the miniaturization of instrumental configurations used for quantitative analysis, aligning with the principles of sustainability.One major challenge lies in the inherent complexity of liquid chromatography systems, which comprise multiple interconnected components. Each component has been subject to miniaturization, with careful attention to preserving analytical sensitivity, selectivity, and accuracy when applied to real samples. Interest in miniaturized systems is further fueled by the theoretical principle that reducing flow rates enhances the surface-to-volume ratio, thereby improving detection sensitivity.Building on this theoretical foundation—particularly the goals of portability, efficient sampling, and on site analysis—innovative approaches such as microfluidics and 3D printing have been employed to develop compact fluidic systems. These technologies not only broaden the scope of liquid chromatography but also open new frontiers, such as drug testing on engineered tissues, and the development of new devices suitable for space missions.This review aims to present a comprehensive overview of the challenges encountered and the advancements achieved in the miniaturization of instrumental setups and portable systems, encompassing both pre- and post-analytical stages. Furthermore, a discussion on 3D technologies available for LC-chip and miniaturized systems was also presented.http://www.sciencedirect.com/science/article/pii/S2772391725000398Miniaturization3D printed systemPortable instrumentationGreen analytical chemistry and green sample preparationLab-on-a-chip
spellingShingle Miryam Perrucci
Imran Ali
Fotouh R. Mansour
Halil I. Ulusoy
Songül Ulusoy
Abuzar Kabir
Ornella Abollino
Agnese Giacomino
Paolo Inaudi
Marcello Locatelli
Erika Maria Ricci
Chemical analysis using miniaturized and Portable 3D printed systems: Where are we now?
Journal of Chromatography Open
Miniaturization
3D printed system
Portable instrumentation
Green analytical chemistry and green sample preparation
Lab-on-a-chip
title Chemical analysis using miniaturized and Portable 3D printed systems: Where are we now?
title_full Chemical analysis using miniaturized and Portable 3D printed systems: Where are we now?
title_fullStr Chemical analysis using miniaturized and Portable 3D printed systems: Where are we now?
title_full_unstemmed Chemical analysis using miniaturized and Portable 3D printed systems: Where are we now?
title_short Chemical analysis using miniaturized and Portable 3D printed systems: Where are we now?
title_sort chemical analysis using miniaturized and portable 3d printed systems where are we now
topic Miniaturization
3D printed system
Portable instrumentation
Green analytical chemistry and green sample preparation
Lab-on-a-chip
url http://www.sciencedirect.com/science/article/pii/S2772391725000398
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