Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma

NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein...

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Main Authors: Mara Giavina-Bianchi, Pedro Giavina-Bianchi, Mirian Nacagami Sotto, Alona Muzikansky, Jorge Kalil, Cyro Festa-Neto, Lyn M. Duncan
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Journal of Immunology Research
Online Access:http://dx.doi.org/10.1155/2015/761378
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author Mara Giavina-Bianchi
Pedro Giavina-Bianchi
Mirian Nacagami Sotto
Alona Muzikansky
Jorge Kalil
Cyro Festa-Neto
Lyn M. Duncan
author_facet Mara Giavina-Bianchi
Pedro Giavina-Bianchi
Mirian Nacagami Sotto
Alona Muzikansky
Jorge Kalil
Cyro Festa-Neto
Lyn M. Duncan
author_sort Mara Giavina-Bianchi
collection DOAJ
description NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79), rarely in in situ melanoma (1/10) and not in benign nevi (0/20). Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. NY-ESO-1 expression was associated with increased primary tumor thickness (P=0.007) and inversely correlated with superficial spreading melanoma (P<0.02). NY-ESO-1 expression was also associated with reduced numbers and density of CD3+ tumor infiltrating lymphocytes (P=0.017). When NY-ESO-1 protein was expressed, CD3+ T cells were less diffusely infiltrating the tumor and were more often arranged in small clusters (P=0.010) or as isolated cells (P=0.002) than in large clusters of more than five lymphocytes. No correlation of NY-ESO-1 expression with gender, age, tumor site, ulceration, lymph node sentinel status, or survival was observed. NY-ESO-1 expression in melanoma was associated with tumor progression, including increased tumor thickness, and with reduced tumor infiltrating lymphocytes.
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spelling doaj-art-34c139a5ee5644c99b1a8b349e9265e52025-08-20T03:55:06ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/761378761378Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous MelanomaMara Giavina-Bianchi0Pedro Giavina-Bianchi1Mirian Nacagami Sotto2Alona Muzikansky3Jorge Kalil4Cyro Festa-Neto5Lyn M. Duncan6Department of Dermatology, University of São Paulo, Avenida Dr. Enéas de Carvalho Aguiar 255, 3° Andar, 05403-900 São Paulo, SP, BrazilDivision of Clinical Immunology and Allergy, University of São Paulo, Avenida Dr. Enéas de Carvalho Aguiar 255, 8° Andar, 05403-900 São Paulo, SP, BrazilDepartment of Dermatology, University of São Paulo, Avenida Dr. Enéas de Carvalho Aguiar 255, 3° Andar, 05403-900 São Paulo, SP, BrazilMGH Biostatistics Center, 50 Staniford Street, Suite 560, Boston, MA 02114, USADivision of Clinical Immunology and Allergy, University of São Paulo, Avenida Dr. Enéas de Carvalho Aguiar 255, 8° Andar, 05403-900 São Paulo, SP, BrazilDepartment of Dermatology, University of São Paulo, Avenida Dr. Enéas de Carvalho Aguiar 255, 3° Andar, 05403-900 São Paulo, SP, BrazilDermatopathology Unit, Pathology Service, Massachusetts General Hospital, Harvard Medical School, Warren Building 825, 55 Fruit Street, Boston, MA 02114, USANY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79), rarely in in situ melanoma (1/10) and not in benign nevi (0/20). Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. NY-ESO-1 expression was associated with increased primary tumor thickness (P=0.007) and inversely correlated with superficial spreading melanoma (P<0.02). NY-ESO-1 expression was also associated with reduced numbers and density of CD3+ tumor infiltrating lymphocytes (P=0.017). When NY-ESO-1 protein was expressed, CD3+ T cells were less diffusely infiltrating the tumor and were more often arranged in small clusters (P=0.010) or as isolated cells (P=0.002) than in large clusters of more than five lymphocytes. No correlation of NY-ESO-1 expression with gender, age, tumor site, ulceration, lymph node sentinel status, or survival was observed. NY-ESO-1 expression in melanoma was associated with tumor progression, including increased tumor thickness, and with reduced tumor infiltrating lymphocytes.http://dx.doi.org/10.1155/2015/761378
spellingShingle Mara Giavina-Bianchi
Pedro Giavina-Bianchi
Mirian Nacagami Sotto
Alona Muzikansky
Jorge Kalil
Cyro Festa-Neto
Lyn M. Duncan
Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma
Journal of Immunology Research
title Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma
title_full Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma
title_fullStr Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma
title_full_unstemmed Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma
title_short Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma
title_sort increased ny eso 1 expression and reduced infiltrating cd3 t cells in cutaneous melanoma
url http://dx.doi.org/10.1155/2015/761378
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