Caffeic acid-vanadium nanozymes treat skin flap ischemia-reperfusion injury through macrophage reprogramming and the upregulation of X-linked inhibitors of apoptotic proteins
Ischemia-reperfusion (I/R) injury following skin flap transplantation is a critical factor leading to flap necrosis and transplant failure. Antagonizing inflammatory responses and oxidative stress are regarded as crucial targets for mitigating reperfusion injury and enhancing flap survival. In this...
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Elsevier
2025-01-01
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| Series: | Acta Pharmaceutica Sinica B |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221138352400340X |
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| author | Xinyu Zhao Jie Shan Hanying Qian Xu Jin Yiwei Sun Jianghao Xing Qingrong Li Xu-Lin Chen Xianwen Wang |
| author_facet | Xinyu Zhao Jie Shan Hanying Qian Xu Jin Yiwei Sun Jianghao Xing Qingrong Li Xu-Lin Chen Xianwen Wang |
| author_sort | Xinyu Zhao |
| collection | DOAJ |
| description | Ischemia-reperfusion (I/R) injury following skin flap transplantation is a critical factor leading to flap necrosis and transplant failure. Antagonizing inflammatory responses and oxidative stress are regarded as crucial targets for mitigating reperfusion injury and enhancing flap survival. In this study, caffeic acid-vanadium metal polyphenol nanoparticles (CA-V NPs) were prepared for the treatment of skin flap ischemia and reperfusion. This study was conducted using a one-step method to prepare new types of CA-V NPs with uniform sizes and stable structures. In vitro, the CA-V NPs exhibited CAT-like and SOD-like activities and could effectively scavenge ROS, generate oxygen, and alleviate oxidative stress. In the H2O2-induced cellular oxidative stress model, CA-V NPs effectively reduced ROS levels and inhibited apoptosis through the XIAP/Caspase-3 pathway. In the cellular inflammation model induced by LPS combined with IFN-γ, CA-V NPs reprogrammed macrophage polarization toward the M2 phenotype and reduced inflammatory responses by reducing the expression of the chemokines CCL4 and CXCL2. In addition, animal experiments have shown that CA-V NPs can alleviate oxidative stress in skin flap tissues, inhibit apoptosis, promote angiogenesis, and ultimately improve the survival rate of skin flaps. CA-V NPs provide a new target and strategy for the treatment of flap I/R injury. |
| format | Article |
| id | doaj-art-34bab274647648ec9237e8b6486edfe2 |
| institution | DOAJ |
| issn | 2211-3835 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj-art-34bab274647648ec9237e8b6486edfe22025-08-20T03:12:38ZengElsevierActa Pharmaceutica Sinica B2211-38352025-01-0115159261010.1016/j.apsb.2024.08.022Caffeic acid-vanadium nanozymes treat skin flap ischemia-reperfusion injury through macrophage reprogramming and the upregulation of X-linked inhibitors of apoptotic proteinsXinyu Zhao0Jie Shan1Hanying Qian2Xu Jin3Yiwei Sun4Jianghao Xing5Qingrong Li6Xu-Lin Chen7Xianwen Wang8Department of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, ChinaDepartment of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, ChinaDepartment of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China; Department of Graduate School, Anhui University of Chinese Medicine, Hefei 230022, ChinaDepartment of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, ChinaSchool of Biomedical Engineering, Anhui Medical University, Hefei 230032, ChinaSchool of Biomedical Engineering, Anhui Medical University, Hefei 230032, ChinaSchool of Biomedical Engineering, Anhui Medical University, Hefei 230032, ChinaDepartment of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China; Corresponding authors.School of Biomedical Engineering, Anhui Medical University, Hefei 230032, China; Corresponding authors.Ischemia-reperfusion (I/R) injury following skin flap transplantation is a critical factor leading to flap necrosis and transplant failure. Antagonizing inflammatory responses and oxidative stress are regarded as crucial targets for mitigating reperfusion injury and enhancing flap survival. In this study, caffeic acid-vanadium metal polyphenol nanoparticles (CA-V NPs) were prepared for the treatment of skin flap ischemia and reperfusion. This study was conducted using a one-step method to prepare new types of CA-V NPs with uniform sizes and stable structures. In vitro, the CA-V NPs exhibited CAT-like and SOD-like activities and could effectively scavenge ROS, generate oxygen, and alleviate oxidative stress. In the H2O2-induced cellular oxidative stress model, CA-V NPs effectively reduced ROS levels and inhibited apoptosis through the XIAP/Caspase-3 pathway. In the cellular inflammation model induced by LPS combined with IFN-γ, CA-V NPs reprogrammed macrophage polarization toward the M2 phenotype and reduced inflammatory responses by reducing the expression of the chemokines CCL4 and CXCL2. In addition, animal experiments have shown that CA-V NPs can alleviate oxidative stress in skin flap tissues, inhibit apoptosis, promote angiogenesis, and ultimately improve the survival rate of skin flaps. CA-V NPs provide a new target and strategy for the treatment of flap I/R injury.http://www.sciencedirect.com/science/article/pii/S221138352400340XCaffeic acid-vanadiumNanozymesSkin flapIschemia-reperfusionOxidative stressInflammation |
| spellingShingle | Xinyu Zhao Jie Shan Hanying Qian Xu Jin Yiwei Sun Jianghao Xing Qingrong Li Xu-Lin Chen Xianwen Wang Caffeic acid-vanadium nanozymes treat skin flap ischemia-reperfusion injury through macrophage reprogramming and the upregulation of X-linked inhibitors of apoptotic proteins Acta Pharmaceutica Sinica B Caffeic acid-vanadium Nanozymes Skin flap Ischemia-reperfusion Oxidative stress Inflammation |
| title | Caffeic acid-vanadium nanozymes treat skin flap ischemia-reperfusion injury through macrophage reprogramming and the upregulation of X-linked inhibitors of apoptotic proteins |
| title_full | Caffeic acid-vanadium nanozymes treat skin flap ischemia-reperfusion injury through macrophage reprogramming and the upregulation of X-linked inhibitors of apoptotic proteins |
| title_fullStr | Caffeic acid-vanadium nanozymes treat skin flap ischemia-reperfusion injury through macrophage reprogramming and the upregulation of X-linked inhibitors of apoptotic proteins |
| title_full_unstemmed | Caffeic acid-vanadium nanozymes treat skin flap ischemia-reperfusion injury through macrophage reprogramming and the upregulation of X-linked inhibitors of apoptotic proteins |
| title_short | Caffeic acid-vanadium nanozymes treat skin flap ischemia-reperfusion injury through macrophage reprogramming and the upregulation of X-linked inhibitors of apoptotic proteins |
| title_sort | caffeic acid vanadium nanozymes treat skin flap ischemia reperfusion injury through macrophage reprogramming and the upregulation of x linked inhibitors of apoptotic proteins |
| topic | Caffeic acid-vanadium Nanozymes Skin flap Ischemia-reperfusion Oxidative stress Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S221138352400340X |
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