Molecular Diagnosis and Therapy of Dental Caries by Oral Microbiome‐Selective Aggregation‐Induced Photosensitivity
ABSTRACT Dental caries represents one of the most widespread oral bacterial infections, affecting billions of individuals worldwide and presenting significant public health challenges. Effective diagnosis and treatment are hindered by the limitations of traditional clinical treatment methodologies,...
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2025-04-01
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| Online Access: | https://doi.org/10.1002/agt2.733 |
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| author | Yandi Zhang Wenjun Wang Xiaoru Yang Xuanmeng An Xiaoyi Liu Weiwei Zhao Lina Zhu Ting Wang Ying Wang Yi Chen Junkun Feng Jinlong Shao Xuedong Zhou Ben Zhong Tang Shaohua Ge Jianhua Li |
| author_facet | Yandi Zhang Wenjun Wang Xiaoru Yang Xuanmeng An Xiaoyi Liu Weiwei Zhao Lina Zhu Ting Wang Ying Wang Yi Chen Junkun Feng Jinlong Shao Xuedong Zhou Ben Zhong Tang Shaohua Ge Jianhua Li |
| author_sort | Yandi Zhang |
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| description | ABSTRACT Dental caries represents one of the most widespread oral bacterial infections, affecting billions of individuals worldwide and presenting significant public health challenges. Effective diagnosis and treatment are hindered by the limitations of traditional clinical treatment methodologies, which often involve laborious examinations and invasive procedures. In this study, we employ an aggregation‐induced emission probe, MeOTpy, for instant molecular detection and photodynamic treatment of dental caries. MeOTpy interacts specifically with cariogenic bacteria, exhibiting bright fluorescence upon binding to bacteria and generating reactive oxygen species under white light irradiation. This aggregation‐induced photosensitivity enables rapid assessment of carious disease through fluorescent detection in dental plaque samples, facilitating easy identification of lesion sites in decayed teeth treated with MeOTpy. Furthermore, photodynamic inhibition of cariogenic biofilms is achieved by culturing ex vivo biofilms isolated from children with severe early childhood caries. MeOTpy also effectively prevents dental caries while preserving oral microbial diversity in a cariogenic infection model on rat pups. This study presents an integrated strategy for the precise diagnosis and treatment of dental caries at the point of care. |
| format | Article |
| id | doaj-art-34ae9811dc7e404d8ef49cd6de21e6b3 |
| institution | OA Journals |
| issn | 2692-4560 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aggregate |
| spelling | doaj-art-34ae9811dc7e404d8ef49cd6de21e6b32025-08-20T02:27:28ZengWileyAggregate2692-45602025-04-0164n/an/a10.1002/agt2.733Molecular Diagnosis and Therapy of Dental Caries by Oral Microbiome‐Selective Aggregation‐Induced PhotosensitivityYandi Zhang0Wenjun Wang1Xiaoru Yang2Xuanmeng An3Xiaoyi Liu4Weiwei Zhao5Lina Zhu6Ting Wang7Ying Wang8Yi Chen9Junkun Feng10Jinlong Shao11Xuedong Zhou12Ben Zhong Tang13Shaohua Ge14Jianhua Li15Department of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaState Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu ChinaShenzhen Institute of Molecular Aggregate Science and Engineering School of Science and Engineering The Chinese University of Hong Kong, Shenzhen Shenzhen City Guangdong ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaDepartment of Biomaterials School and Hospital of Stomatology Cheeloo College of Medicine Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases Jinan ChinaABSTRACT Dental caries represents one of the most widespread oral bacterial infections, affecting billions of individuals worldwide and presenting significant public health challenges. Effective diagnosis and treatment are hindered by the limitations of traditional clinical treatment methodologies, which often involve laborious examinations and invasive procedures. In this study, we employ an aggregation‐induced emission probe, MeOTpy, for instant molecular detection and photodynamic treatment of dental caries. MeOTpy interacts specifically with cariogenic bacteria, exhibiting bright fluorescence upon binding to bacteria and generating reactive oxygen species under white light irradiation. This aggregation‐induced photosensitivity enables rapid assessment of carious disease through fluorescent detection in dental plaque samples, facilitating easy identification of lesion sites in decayed teeth treated with MeOTpy. Furthermore, photodynamic inhibition of cariogenic biofilms is achieved by culturing ex vivo biofilms isolated from children with severe early childhood caries. MeOTpy also effectively prevents dental caries while preserving oral microbial diversity in a cariogenic infection model on rat pups. This study presents an integrated strategy for the precise diagnosis and treatment of dental caries at the point of care.https://doi.org/10.1002/agt2.733aggregation‐induced emissionbiofilmcariesmolecular diagnosisoral microbiomephotodynamic therapy |
| spellingShingle | Yandi Zhang Wenjun Wang Xiaoru Yang Xuanmeng An Xiaoyi Liu Weiwei Zhao Lina Zhu Ting Wang Ying Wang Yi Chen Junkun Feng Jinlong Shao Xuedong Zhou Ben Zhong Tang Shaohua Ge Jianhua Li Molecular Diagnosis and Therapy of Dental Caries by Oral Microbiome‐Selective Aggregation‐Induced Photosensitivity Aggregate aggregation‐induced emission biofilm caries molecular diagnosis oral microbiome photodynamic therapy |
| title | Molecular Diagnosis and Therapy of Dental Caries by Oral Microbiome‐Selective Aggregation‐Induced Photosensitivity |
| title_full | Molecular Diagnosis and Therapy of Dental Caries by Oral Microbiome‐Selective Aggregation‐Induced Photosensitivity |
| title_fullStr | Molecular Diagnosis and Therapy of Dental Caries by Oral Microbiome‐Selective Aggregation‐Induced Photosensitivity |
| title_full_unstemmed | Molecular Diagnosis and Therapy of Dental Caries by Oral Microbiome‐Selective Aggregation‐Induced Photosensitivity |
| title_short | Molecular Diagnosis and Therapy of Dental Caries by Oral Microbiome‐Selective Aggregation‐Induced Photosensitivity |
| title_sort | molecular diagnosis and therapy of dental caries by oral microbiome selective aggregation induced photosensitivity |
| topic | aggregation‐induced emission biofilm caries molecular diagnosis oral microbiome photodynamic therapy |
| url | https://doi.org/10.1002/agt2.733 |
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