Achromobacter xylosoxidans modulates Pseudomonas aeruginosa virulence through a complex multi-target competition
Abstract The colonization and persistence of Pseudomonas aeruginosa in chronically diseased lungs are driven by various virulence factors. However, pulmonary infections in cystic fibrosis (CF) patients are predominantly polymicrobial. While Achromobacter xylosoxidans is an opportunistic pathogen in...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-06075-w |
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| author | Alison Besse Quentin Menetrey Vincent Jean-Pierre Sylvaine Huc-Brandt Fabien Aujoulat Chloé Dupont Raphaël Chiron Jean Armengaud Estelle Jumas-Bilak Virginie Molle Lucia Grenga Hélène Marchandin |
| author_facet | Alison Besse Quentin Menetrey Vincent Jean-Pierre Sylvaine Huc-Brandt Fabien Aujoulat Chloé Dupont Raphaël Chiron Jean Armengaud Estelle Jumas-Bilak Virginie Molle Lucia Grenga Hélène Marchandin |
| author_sort | Alison Besse |
| collection | DOAJ |
| description | Abstract The colonization and persistence of Pseudomonas aeruginosa in chronically diseased lungs are driven by various virulence factors. However, pulmonary infections in cystic fibrosis (CF) patients are predominantly polymicrobial. While Achromobacter xylosoxidans is an opportunistic pathogen in these patients, its impact on P. aeruginosa virulence during co-infection remains largely unknown. This study investigated P. aeruginosa interaction with two clonally related A. xylosoxidans strains, Ax 198 and Ax 200, co-isolated from CF sputum. We found that the interaction was strain-dependent, with Ax 200 significantly reducing P. aeruginosa virulence in a zebrafish model, providing the first in vivo evidence of this interaction. Proteomic analysis revealed that P. aeruginosa proteome was differently impacted by the two A. xylosoxidans strains, with Ax 200 altering proteins involved in biofilm formation, swimming motility, iron acquisition, and secretion systems. These findings were validated by phenotypic assays, confirming that A. xylosoxidans affected major P. aeruginosa virulence phenotypes, including biofilm formation, swimming motility, and siderophore production. Genetic analysis confirmed that distinct regulatory mechanisms, including iron cycle pathways, may account for the strain-dependent effects. These findings reveal a novel multi-target competitive mechanism through which A. xylosoxidans significantly disrupts P. aeruginosa virulence. |
| format | Article |
| id | doaj-art-3482f9e2005c4d8f943dc1c0a1e463d1 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-3482f9e2005c4d8f943dc1c0a1e463d12025-08-20T03:38:16ZengNature PortfolioScientific Reports2045-23222025-07-0115111310.1038/s41598-025-06075-wAchromobacter xylosoxidans modulates Pseudomonas aeruginosa virulence through a complex multi-target competitionAlison Besse0Quentin Menetrey1Vincent Jean-Pierre2Sylvaine Huc-Brandt3Fabien Aujoulat4Chloé Dupont5Raphaël Chiron6Jean Armengaud7Estelle Jumas-Bilak8Virginie Molle9Lucia Grenga10Hélène Marchandin11HydroSciences Montpellier, Univ Montpellier, CNRS, IRDINFINITE-Institute for Translational Research in Inflammation, Univ Lille, INSERMHydroSciences Montpellier, Univ Montpellier, CNRS, IRD, Service de Microbiologie et Hygiène Hospitalière, CHU NîmesLaboratory of Pathogens and Host Immunity (LPHI) UMR5294, University of Montpellier, CNRS, INSERMHydroSciences Montpellier, Univ Montpellier, CNRS, IRDMIVEGEC, IRD, CNRS, Univ Montpellier, Laboratoire de Bactériologie, CHU MontpellierHydroSciences Montpellier, Univ Montpellier, CNRS, IRD, Centre de Ressources et de Compétences de la Mucoviscidose, CHU MontpellierUniversité Paris-Saclay, Département Médicaments et Technologies pour la Santé, CEA, INRAE, SPIHydroSciences Montpellier, Univ Montpellier, CNRS, IRD, Laboratoire d’Écologie Microbienne Hospitalière, CHU MontpellierVBIC, INSERM U1047, University of MontpellierUniversité Paris-Saclay, Département Médicaments et Technologies pour la Santé, CEA, INRAE, SPIHydroSciences Montpellier, Univ Montpellier, CNRS, IRD, Service de Microbiologie et Hygiène Hospitalière, CHU NîmesAbstract The colonization and persistence of Pseudomonas aeruginosa in chronically diseased lungs are driven by various virulence factors. However, pulmonary infections in cystic fibrosis (CF) patients are predominantly polymicrobial. While Achromobacter xylosoxidans is an opportunistic pathogen in these patients, its impact on P. aeruginosa virulence during co-infection remains largely unknown. This study investigated P. aeruginosa interaction with two clonally related A. xylosoxidans strains, Ax 198 and Ax 200, co-isolated from CF sputum. We found that the interaction was strain-dependent, with Ax 200 significantly reducing P. aeruginosa virulence in a zebrafish model, providing the first in vivo evidence of this interaction. Proteomic analysis revealed that P. aeruginosa proteome was differently impacted by the two A. xylosoxidans strains, with Ax 200 altering proteins involved in biofilm formation, swimming motility, iron acquisition, and secretion systems. These findings were validated by phenotypic assays, confirming that A. xylosoxidans affected major P. aeruginosa virulence phenotypes, including biofilm formation, swimming motility, and siderophore production. Genetic analysis confirmed that distinct regulatory mechanisms, including iron cycle pathways, may account for the strain-dependent effects. These findings reveal a novel multi-target competitive mechanism through which A. xylosoxidans significantly disrupts P. aeruginosa virulence.https://doi.org/10.1038/s41598-025-06075-wP. aeruginosaInter-bacterial interactionVirulenceCystic fibrosisA. xylosoxidans |
| spellingShingle | Alison Besse Quentin Menetrey Vincent Jean-Pierre Sylvaine Huc-Brandt Fabien Aujoulat Chloé Dupont Raphaël Chiron Jean Armengaud Estelle Jumas-Bilak Virginie Molle Lucia Grenga Hélène Marchandin Achromobacter xylosoxidans modulates Pseudomonas aeruginosa virulence through a complex multi-target competition Scientific Reports P. aeruginosa Inter-bacterial interaction Virulence Cystic fibrosis A. xylosoxidans |
| title | Achromobacter xylosoxidans modulates Pseudomonas aeruginosa virulence through a complex multi-target competition |
| title_full | Achromobacter xylosoxidans modulates Pseudomonas aeruginosa virulence through a complex multi-target competition |
| title_fullStr | Achromobacter xylosoxidans modulates Pseudomonas aeruginosa virulence through a complex multi-target competition |
| title_full_unstemmed | Achromobacter xylosoxidans modulates Pseudomonas aeruginosa virulence through a complex multi-target competition |
| title_short | Achromobacter xylosoxidans modulates Pseudomonas aeruginosa virulence through a complex multi-target competition |
| title_sort | achromobacter xylosoxidans modulates pseudomonas aeruginosa virulence through a complex multi target competition |
| topic | P. aeruginosa Inter-bacterial interaction Virulence Cystic fibrosis A. xylosoxidans |
| url | https://doi.org/10.1038/s41598-025-06075-w |
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