Antimicrobial resistance of Escherichia coli from piglets with pre-weaning diarrhea and analysis of its enterobacterial repetitive intergenic consensus fingerprinting profiles
In order to investigate the relationship between fingerprinting and antimicrobial resistance patterns, 47 isolates of Escherichia coli from piglets with pre-weaning diarrhea from 2 local large piggeries were collected for susceptibility testing by Kirby-Bauer method and studied the presence of integ...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2010-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.02.002 |
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| Summary: | In order to investigate the relationship between fingerprinting and antimicrobial resistance patterns, 47 isolates of Escherichia coli from piglets with pre-weaning diarrhea from 2 local large piggeries were collected for susceptibility testing by Kirby-Bauer method and studied the presence of integron integrase gene by polymerase chain reaction (PCR), respectively. The isolates with different phenotypes were then genotyped by enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting and further clustered on the basis of the profiles using statistical software SPSS 16.0. The results showed that the 47 isolates were all multi-drug resistant and exhibited 9 phenotypes, whereas 45 of the 47 isolates (95.7%) were integron I positive. ERIC-PCR was found to generate the reproducible fingerprinting profiles; two dominant profiles were shown in fingerprints of isolates resistant to 9-13 antimicrobial agents, each standing for isolates from the relevant pig farm. The cluster analysis demonstrated that the similarity among the isolates with the same patterns of resistance from the same piggery and different piggeries was 68.7% and 53.5%, respectively, significantly (P<0.05) higher than that of 47 isolates (37.3%). It is indicated that specific ERIC fingerprints might have relations with resistance pattern, and ERIC-PCR fingerprinting could be applied as a novel tool to the characterizations of multi-resistant phenotypes and genotypes of E. coli from piglets with diarrhea. |
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| ISSN: | 1008-9209 2097-5155 |