Genome-Wide Identification of β-Ketoacyl CoA Synthase Gene Family in Melon (<i>Cucumis melo</i> L.) and Its Expression Analysis in Autotoxicity, Saline-Alkali, and Microplastic Exposure Environments
β-ketoacyl CoA synthase (KCS) is a key enzyme in the synthesis of long-chain fatty acids. It affects plant stress resistance by regulating the chain length of fatty acid elongation products, the wax deposition in plant epidermis, and the formation of suberization layers. Through a comprehensive, gen...
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2025-03-01
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| author | Lizhen Zhang Mingcheng Wang Xianhuan Tang Xinyue Yang Zhizhong Zhang Jinghua Wu |
| author_facet | Lizhen Zhang Mingcheng Wang Xianhuan Tang Xinyue Yang Zhizhong Zhang Jinghua Wu |
| author_sort | Lizhen Zhang |
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| description | β-ketoacyl CoA synthase (KCS) is a key enzyme in the synthesis of long-chain fatty acids. It affects plant stress resistance by regulating the chain length of fatty acid elongation products, the wax deposition in plant epidermis, and the formation of suberization layers. Through a comprehensive, genome-wide analysis, we identified members of the melon <i>KCS</i> (<i>CmKCS</i>) family and characterized their sequence features, phylogenetic relationships, and expression profiles under three abiotic stress conditions, employing bioinformatics tools and methods. Fifteen <i>CmKCSs</i> were identified in the melon genome and found to be unevenly distributed across eight chromosomes. The subcellular localization of most members is located on the cytoplasmic membrane and chloroplasts. The <i>CmKCS</i> family amplifies its members in a tandem repeat manner, which is more closely related to the cucumber <i>KCS</i> and has similar gene functions. Subfamilies I, IV, and VI exhibit variations in conserved domain sequences, which may indicate specific functional differentiation. The promoter region harbors various cis-acting elements related to plant hormones and abiotic stress responses. Among these, the most abundant are elements responsive to abscisic acid, methyl jasmonate, salicylic acid, and anaerobic induction. <i>CmKCS5</i>, <i>CmKCS6</i>, <i>CmKCS10</i>, and <i>CmKCS12</i> showed high expression in autotoxicity, saline-alkali stress, and microplastic exposure environments. These four <i>CmKCSs</i> may play important roles in melon development and stress response. In conclusion, this study provides a comprehensive analysis of the <i>CmKCS</i> gene family, revealing its potential roles in melon’s response to abiotic stresses and laying a foundation for further functional characterization of these genes in stress tolerance mechanisms. |
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| spelling | doaj-art-346e6e774a4d41658f750db2f06d29662025-08-20T02:11:16ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-03-0147319510.3390/cimb47030195Genome-Wide Identification of β-Ketoacyl CoA Synthase Gene Family in Melon (<i>Cucumis melo</i> L.) and Its Expression Analysis in Autotoxicity, Saline-Alkali, and Microplastic Exposure EnvironmentsLizhen Zhang0Mingcheng Wang1Xianhuan Tang2Xinyue Yang3Zhizhong Zhang4Jinghua Wu5College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Chinaβ-ketoacyl CoA synthase (KCS) is a key enzyme in the synthesis of long-chain fatty acids. It affects plant stress resistance by regulating the chain length of fatty acid elongation products, the wax deposition in plant epidermis, and the formation of suberization layers. Through a comprehensive, genome-wide analysis, we identified members of the melon <i>KCS</i> (<i>CmKCS</i>) family and characterized their sequence features, phylogenetic relationships, and expression profiles under three abiotic stress conditions, employing bioinformatics tools and methods. Fifteen <i>CmKCSs</i> were identified in the melon genome and found to be unevenly distributed across eight chromosomes. The subcellular localization of most members is located on the cytoplasmic membrane and chloroplasts. The <i>CmKCS</i> family amplifies its members in a tandem repeat manner, which is more closely related to the cucumber <i>KCS</i> and has similar gene functions. Subfamilies I, IV, and VI exhibit variations in conserved domain sequences, which may indicate specific functional differentiation. The promoter region harbors various cis-acting elements related to plant hormones and abiotic stress responses. Among these, the most abundant are elements responsive to abscisic acid, methyl jasmonate, salicylic acid, and anaerobic induction. <i>CmKCS5</i>, <i>CmKCS6</i>, <i>CmKCS10</i>, and <i>CmKCS12</i> showed high expression in autotoxicity, saline-alkali stress, and microplastic exposure environments. These four <i>CmKCSs</i> may play important roles in melon development and stress response. In conclusion, this study provides a comprehensive analysis of the <i>CmKCS</i> gene family, revealing its potential roles in melon’s response to abiotic stresses and laying a foundation for further functional characterization of these genes in stress tolerance mechanisms.https://www.mdpi.com/1467-3045/47/3/195β-ketoacyl CoA synthase genecucurbitsmolecular phylogenystress response genevery-long-chain fatty acids |
| spellingShingle | Lizhen Zhang Mingcheng Wang Xianhuan Tang Xinyue Yang Zhizhong Zhang Jinghua Wu Genome-Wide Identification of β-Ketoacyl CoA Synthase Gene Family in Melon (<i>Cucumis melo</i> L.) and Its Expression Analysis in Autotoxicity, Saline-Alkali, and Microplastic Exposure Environments Current Issues in Molecular Biology β-ketoacyl CoA synthase gene cucurbits molecular phylogeny stress response gene very-long-chain fatty acids |
| title | Genome-Wide Identification of β-Ketoacyl CoA Synthase Gene Family in Melon (<i>Cucumis melo</i> L.) and Its Expression Analysis in Autotoxicity, Saline-Alkali, and Microplastic Exposure Environments |
| title_full | Genome-Wide Identification of β-Ketoacyl CoA Synthase Gene Family in Melon (<i>Cucumis melo</i> L.) and Its Expression Analysis in Autotoxicity, Saline-Alkali, and Microplastic Exposure Environments |
| title_fullStr | Genome-Wide Identification of β-Ketoacyl CoA Synthase Gene Family in Melon (<i>Cucumis melo</i> L.) and Its Expression Analysis in Autotoxicity, Saline-Alkali, and Microplastic Exposure Environments |
| title_full_unstemmed | Genome-Wide Identification of β-Ketoacyl CoA Synthase Gene Family in Melon (<i>Cucumis melo</i> L.) and Its Expression Analysis in Autotoxicity, Saline-Alkali, and Microplastic Exposure Environments |
| title_short | Genome-Wide Identification of β-Ketoacyl CoA Synthase Gene Family in Melon (<i>Cucumis melo</i> L.) and Its Expression Analysis in Autotoxicity, Saline-Alkali, and Microplastic Exposure Environments |
| title_sort | genome wide identification of β ketoacyl coa synthase gene family in melon i cucumis melo i l and its expression analysis in autotoxicity saline alkali and microplastic exposure environments |
| topic | β-ketoacyl CoA synthase gene cucurbits molecular phylogeny stress response gene very-long-chain fatty acids |
| url | https://www.mdpi.com/1467-3045/47/3/195 |
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