CeDaD—a novel assay for simultaneous tracking of cell death and division in a single population
Abstract The cell division cycle and the various forms of programmed cell death are interconnected. A prominent example is the tumor suppressor p53, which not only induces apoptosis but also plays an important role in the arrest of the cell cycle. Consequently, simultaneous analysis of cell division...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-03-01
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| Series: | Cell Death Discovery |
| Online Access: | https://doi.org/10.1038/s41420-025-02370-7 |
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| _version_ | 1850029932548194304 |
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| author | Lukas Nöltner Kurt Engeland Robin Kohler |
| author_facet | Lukas Nöltner Kurt Engeland Robin Kohler |
| author_sort | Lukas Nöltner |
| collection | DOAJ |
| description | Abstract The cell division cycle and the various forms of programmed cell death are interconnected. A prominent example is the tumor suppressor p53, which not only induces apoptosis but also plays an important role in the arrest of the cell cycle. Consequently, simultaneous analysis of cell division and cell death is frequently of significant interest in cell biology research. Traditionally, these processes require distinct assays, making concurrent analysis challenging. To address this, we present a novel combined assay, called CeDaD assay—Cell Death and Division assay—which allows for the simultaneous quantification of cell division and cell death within a single-cell population. This assay utilizes a straightforward flow cytometric approach, combining a staining based on carboxyfluorescein succinimidyl ester (CFSE) to monitor cell division with an annexin V-derived staining to assess the extent of cell death. |
| format | Article |
| id | doaj-art-346d25f452df45199a607e1bbbb537d2 |
| institution | DOAJ |
| issn | 2058-7716 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death Discovery |
| spelling | doaj-art-346d25f452df45199a607e1bbbb537d22025-08-20T02:59:23ZengNature Publishing GroupCell Death Discovery2058-77162025-03-011111710.1038/s41420-025-02370-7CeDaD—a novel assay for simultaneous tracking of cell death and division in a single populationLukas Nöltner0Kurt Engeland1Robin Kohler2Molecular Oncology, Faculty of Medicine, University of LeipzigMolecular Oncology, Faculty of Medicine, University of LeipzigMolecular Oncology, Faculty of Medicine, University of LeipzigAbstract The cell division cycle and the various forms of programmed cell death are interconnected. A prominent example is the tumor suppressor p53, which not only induces apoptosis but also plays an important role in the arrest of the cell cycle. Consequently, simultaneous analysis of cell division and cell death is frequently of significant interest in cell biology research. Traditionally, these processes require distinct assays, making concurrent analysis challenging. To address this, we present a novel combined assay, called CeDaD assay—Cell Death and Division assay—which allows for the simultaneous quantification of cell division and cell death within a single-cell population. This assay utilizes a straightforward flow cytometric approach, combining a staining based on carboxyfluorescein succinimidyl ester (CFSE) to monitor cell division with an annexin V-derived staining to assess the extent of cell death.https://doi.org/10.1038/s41420-025-02370-7 |
| spellingShingle | Lukas Nöltner Kurt Engeland Robin Kohler CeDaD—a novel assay for simultaneous tracking of cell death and division in a single population Cell Death Discovery |
| title | CeDaD—a novel assay for simultaneous tracking of cell death and division in a single population |
| title_full | CeDaD—a novel assay for simultaneous tracking of cell death and division in a single population |
| title_fullStr | CeDaD—a novel assay for simultaneous tracking of cell death and division in a single population |
| title_full_unstemmed | CeDaD—a novel assay for simultaneous tracking of cell death and division in a single population |
| title_short | CeDaD—a novel assay for simultaneous tracking of cell death and division in a single population |
| title_sort | cedad a novel assay for simultaneous tracking of cell death and division in a single population |
| url | https://doi.org/10.1038/s41420-025-02370-7 |
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