Advances in In Vitro and In Vivo Bioreactor-Based Bone Generation for Craniofacial Tissue Engineering
Craniofacial reconstruction requires robust bone of specified geometry for the repair to be both functional and aesthetic. While native bone from elsewhere in the body can be harvested, shaped, and implanted within a defect, using either an in vitro or in vivo bioreactors eliminates donor site morbi...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2023-01-01
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| Series: | BME Frontiers |
| Online Access: | https://spj.science.org/doi/10.34133/bmef.0004 |
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| author | Emma Watson Antonios G. Mikos |
| author_facet | Emma Watson Antonios G. Mikos |
| author_sort | Emma Watson |
| collection | DOAJ |
| description | Craniofacial reconstruction requires robust bone of specified geometry for the repair to be both functional and aesthetic. While native bone from elsewhere in the body can be harvested, shaped, and implanted within a defect, using either an in vitro or in vivo bioreactors eliminates donor site morbidity while increasing the customizability of the generated tissue. In vitro bioreactors utilize cells harvested from the patient, a scaffold, and a device to increase mass transfer of nutrients, oxygen, and waste, allowing for generation of larger viable tissues. In vivo bioreactors utilize the patient’s own body as a source of cells and of nutrient transfer and involve the implantation of a scaffold with or without growth factors adjacent to vasculature, followed by the eventual transfer of vascularized, mineralized tissue to the defect site. Several different models of in vitro bioreactors exist, and several different implantation sites have been successfully utilized for in vivo tissue generation and defect repair in humans. In this review, we discuss the specifics of each bioreactor strategy, as well as the advantages and disadvantages of each and the future directions for the engineering of bony tissues for craniofacial defect repair. |
| format | Article |
| id | doaj-art-34680811aefd444aaa3626499725d2f4 |
| institution | DOAJ |
| issn | 2765-8031 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | BME Frontiers |
| spelling | doaj-art-34680811aefd444aaa3626499725d2f42025-08-20T03:10:54ZengAmerican Association for the Advancement of Science (AAAS)BME Frontiers2765-80312023-01-01410.34133/bmef.0004Advances in In Vitro and In Vivo Bioreactor-Based Bone Generation for Craniofacial Tissue EngineeringEmma Watson0Antonios G. Mikos1Department of Bioengineering, Rice University, Houston, TX 77030, USA.Department of Bioengineering, Rice University, Houston, TX 77030, USA.Craniofacial reconstruction requires robust bone of specified geometry for the repair to be both functional and aesthetic. While native bone from elsewhere in the body can be harvested, shaped, and implanted within a defect, using either an in vitro or in vivo bioreactors eliminates donor site morbidity while increasing the customizability of the generated tissue. In vitro bioreactors utilize cells harvested from the patient, a scaffold, and a device to increase mass transfer of nutrients, oxygen, and waste, allowing for generation of larger viable tissues. In vivo bioreactors utilize the patient’s own body as a source of cells and of nutrient transfer and involve the implantation of a scaffold with or without growth factors adjacent to vasculature, followed by the eventual transfer of vascularized, mineralized tissue to the defect site. Several different models of in vitro bioreactors exist, and several different implantation sites have been successfully utilized for in vivo tissue generation and defect repair in humans. In this review, we discuss the specifics of each bioreactor strategy, as well as the advantages and disadvantages of each and the future directions for the engineering of bony tissues for craniofacial defect repair.https://spj.science.org/doi/10.34133/bmef.0004 |
| spellingShingle | Emma Watson Antonios G. Mikos Advances in In Vitro and In Vivo Bioreactor-Based Bone Generation for Craniofacial Tissue Engineering BME Frontiers |
| title | Advances in In Vitro and In Vivo Bioreactor-Based Bone Generation for Craniofacial Tissue Engineering |
| title_full | Advances in In Vitro and In Vivo Bioreactor-Based Bone Generation for Craniofacial Tissue Engineering |
| title_fullStr | Advances in In Vitro and In Vivo Bioreactor-Based Bone Generation for Craniofacial Tissue Engineering |
| title_full_unstemmed | Advances in In Vitro and In Vivo Bioreactor-Based Bone Generation for Craniofacial Tissue Engineering |
| title_short | Advances in In Vitro and In Vivo Bioreactor-Based Bone Generation for Craniofacial Tissue Engineering |
| title_sort | advances in in vitro and in vivo bioreactor based bone generation for craniofacial tissue engineering |
| url | https://spj.science.org/doi/10.34133/bmef.0004 |
| work_keys_str_mv | AT emmawatson advancesininvitroandinvivobioreactorbasedbonegenerationforcraniofacialtissueengineering AT antoniosgmikos advancesininvitroandinvivobioreactorbasedbonegenerationforcraniofacialtissueengineering |