Organoid model of urothelial cancer: establishment and applications for bladder cancer research
3D cancer cell models are suitable for drug evaluation because they more precisely mimic tissue architecture than 2D cultures. To study cytotoxicity of anticancer agents, the most sensitive CellTiter-Glo 3D assay is used. However, this is an end point assay, so it is not possible to consider the var...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-09-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2020-0068 |
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| _version_ | 1850152042277896192 |
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| author | Terry Whyard Jingxuan Liu Frank S Darras Wayne C Waltzer Victor Romanov |
| author_facet | Terry Whyard Jingxuan Liu Frank S Darras Wayne C Waltzer Victor Romanov |
| author_sort | Terry Whyard |
| collection | DOAJ |
| description | 3D cancer cell models are suitable for drug evaluation because they more precisely mimic tissue architecture than 2D cultures. To study cytotoxicity of anticancer agents, the most sensitive CellTiter-Glo 3D assay is used. However, this is an end point assay, so it is not possible to consider the variance of the starting material amount in the final reading. It is difficult to maintain an even plating density of 3D organoids for cytotoxicity analysis. We present a simple, 3D bladder cancer culture that can be maintained, cryopreserved and used for molecular and drug response studies. We applied a simple modification of the drug response assay for 3D cultures by measuring the background signal with the CellTiter Blue assay before drug application. |
| format | Article |
| id | doaj-art-34669c865ba3471b8521074e7fe553c8 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2020-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-34669c865ba3471b8521074e7fe553c82025-08-20T02:26:04ZengTaylor & Francis GroupBioTechniques0736-62051940-98182020-09-0169319319910.2144/btn-2020-0068Organoid model of urothelial cancer: establishment and applications for bladder cancer researchTerry Whyard0Jingxuan Liu1Frank S Darras2Wayne C Waltzer3Victor Romanov41Department of Urology, School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA2Department of Pathology, School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA1Department of Urology, School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA1Department of Urology, School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA1Department of Urology, School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA3D cancer cell models are suitable for drug evaluation because they more precisely mimic tissue architecture than 2D cultures. To study cytotoxicity of anticancer agents, the most sensitive CellTiter-Glo 3D assay is used. However, this is an end point assay, so it is not possible to consider the variance of the starting material amount in the final reading. It is difficult to maintain an even plating density of 3D organoids for cytotoxicity analysis. We present a simple, 3D bladder cancer culture that can be maintained, cryopreserved and used for molecular and drug response studies. We applied a simple modification of the drug response assay for 3D cultures by measuring the background signal with the CellTiter Blue assay before drug application.https://www.future-science.com/doi/10.2144/btn-2020-00683D cell culturebladder cancercell toxicity assaydrug responseorganoid |
| spellingShingle | Terry Whyard Jingxuan Liu Frank S Darras Wayne C Waltzer Victor Romanov Organoid model of urothelial cancer: establishment and applications for bladder cancer research BioTechniques 3D cell culture bladder cancer cell toxicity assay drug response organoid |
| title | Organoid model of urothelial cancer: establishment and applications for bladder cancer research |
| title_full | Organoid model of urothelial cancer: establishment and applications for bladder cancer research |
| title_fullStr | Organoid model of urothelial cancer: establishment and applications for bladder cancer research |
| title_full_unstemmed | Organoid model of urothelial cancer: establishment and applications for bladder cancer research |
| title_short | Organoid model of urothelial cancer: establishment and applications for bladder cancer research |
| title_sort | organoid model of urothelial cancer establishment and applications for bladder cancer research |
| topic | 3D cell culture bladder cancer cell toxicity assay drug response organoid |
| url | https://www.future-science.com/doi/10.2144/btn-2020-0068 |
| work_keys_str_mv | AT terrywhyard organoidmodelofurothelialcancerestablishmentandapplicationsforbladdercancerresearch AT jingxuanliu organoidmodelofurothelialcancerestablishmentandapplicationsforbladdercancerresearch AT franksdarras organoidmodelofurothelialcancerestablishmentandapplicationsforbladdercancerresearch AT waynecwaltzer organoidmodelofurothelialcancerestablishmentandapplicationsforbladdercancerresearch AT victorromanov organoidmodelofurothelialcancerestablishmentandapplicationsforbladdercancerresearch |