Unveiling the Genomic Features and Biocontrol Potential of <i>Trichoderma hamatum</i> Against Root Rot Pathogens
<i>Fusarium</i> species are among the most significant pathogens causing root rot in <i>Panax notoginseng</i>. In this study, a strain of <i>Trichoderma hamatum</i> was isolated from the rhizosphere soil of <i>P. notoginseng</i> and subjected to whole-...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/2/126 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850229777354457088 |
|---|---|
| author | Yuzhou Feng Xinyi Shuai Jili Chen Qing Zhang Lijie Jia Luzhi Sun Yunxia Su Yanyan Su Gangqiang Dong Tao Liu Guangqiang Long |
| author_facet | Yuzhou Feng Xinyi Shuai Jili Chen Qing Zhang Lijie Jia Luzhi Sun Yunxia Su Yanyan Su Gangqiang Dong Tao Liu Guangqiang Long |
| author_sort | Yuzhou Feng |
| collection | DOAJ |
| description | <i>Fusarium</i> species are among the most significant pathogens causing root rot in <i>Panax notoginseng</i>. In this study, a strain of <i>Trichoderma hamatum</i> was isolated from the rhizosphere soil of <i>P. notoginseng</i> and subjected to whole-genome sequencing. Plate confrontation experiments were conducted to investigate the antagonistic effects of <i>T. hamatum</i> against <i>Fusarium oxysporum</i>, <i>Fusarium solani</i>, and <i>Fusarium acutatum</i>, the primary <i>Fusarium</i> species causing root rot. Whole-genome sequencing revealed 10,774 predicted genes in <i>T. hamatum</i>, of which 454 were associated with carbohydrate-active enzymes (CAZymes) involved in fungal cell wall degradation. Additionally, 11 biosynthetic gene clusters (BGCs) associated with antimicrobial production were identified, highlighting the biocontrol potential of <i>T. hamatum</i>. In plate confrontation experiments, <i>T. hamatum</i> showed substantial inhibition rates of 68.07%, 70.63%, and 66.12% against <i>F. oxysporum</i>, <i>F. solani</i>, and <i>F. acutatum</i>, respectively. Scanning electron microscopy suggested the hyperparasitism of <i>T. hamatum</i> against <i>F. solani</i>, which was characterized by spore production that adhered to the pathogen, thereby inhibiting its growth. These findings provide a theoretical foundation to enhance understanding of the biological control mechanisms of <i>T. hamatum</i>, supporting its potential applications in sustainable agriculture. |
| format | Article |
| id | doaj-art-346381dc995045beab981d5f95ccd8fb |
| institution | OA Journals |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-346381dc995045beab981d5f95ccd8fb2025-08-20T02:04:06ZengMDPI AGJournal of Fungi2309-608X2025-02-0111212610.3390/jof11020126Unveiling the Genomic Features and Biocontrol Potential of <i>Trichoderma hamatum</i> Against Root Rot PathogensYuzhou Feng0Xinyi Shuai1Jili Chen2Qing Zhang3Lijie Jia4Luzhi Sun5Yunxia Su6Yanyan Su7Gangqiang Dong8Tao Liu9Guangqiang Long10Key Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming 650201, ChinaNational and Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming 650201, ChinaNational and Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming 650201, ChinaAmway China Botanical R&D Center, Wuxi 214115, ChinaAmway China Botanical R&D Center, Wuxi 214115, ChinaKey Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming 650201, ChinaKey Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming 650201, China<i>Fusarium</i> species are among the most significant pathogens causing root rot in <i>Panax notoginseng</i>. In this study, a strain of <i>Trichoderma hamatum</i> was isolated from the rhizosphere soil of <i>P. notoginseng</i> and subjected to whole-genome sequencing. Plate confrontation experiments were conducted to investigate the antagonistic effects of <i>T. hamatum</i> against <i>Fusarium oxysporum</i>, <i>Fusarium solani</i>, and <i>Fusarium acutatum</i>, the primary <i>Fusarium</i> species causing root rot. Whole-genome sequencing revealed 10,774 predicted genes in <i>T. hamatum</i>, of which 454 were associated with carbohydrate-active enzymes (CAZymes) involved in fungal cell wall degradation. Additionally, 11 biosynthetic gene clusters (BGCs) associated with antimicrobial production were identified, highlighting the biocontrol potential of <i>T. hamatum</i>. In plate confrontation experiments, <i>T. hamatum</i> showed substantial inhibition rates of 68.07%, 70.63%, and 66.12% against <i>F. oxysporum</i>, <i>F. solani</i>, and <i>F. acutatum</i>, respectively. Scanning electron microscopy suggested the hyperparasitism of <i>T. hamatum</i> against <i>F. solani</i>, which was characterized by spore production that adhered to the pathogen, thereby inhibiting its growth. These findings provide a theoretical foundation to enhance understanding of the biological control mechanisms of <i>T. hamatum</i>, supporting its potential applications in sustainable agriculture.https://www.mdpi.com/2309-608X/11/2/126<i>T. hamatum</i><i>Fusarium</i>biological controlgenomeroot rot disease |
| spellingShingle | Yuzhou Feng Xinyi Shuai Jili Chen Qing Zhang Lijie Jia Luzhi Sun Yunxia Su Yanyan Su Gangqiang Dong Tao Liu Guangqiang Long Unveiling the Genomic Features and Biocontrol Potential of <i>Trichoderma hamatum</i> Against Root Rot Pathogens Journal of Fungi <i>T. hamatum</i> <i>Fusarium</i> biological control genome root rot disease |
| title | Unveiling the Genomic Features and Biocontrol Potential of <i>Trichoderma hamatum</i> Against Root Rot Pathogens |
| title_full | Unveiling the Genomic Features and Biocontrol Potential of <i>Trichoderma hamatum</i> Against Root Rot Pathogens |
| title_fullStr | Unveiling the Genomic Features and Biocontrol Potential of <i>Trichoderma hamatum</i> Against Root Rot Pathogens |
| title_full_unstemmed | Unveiling the Genomic Features and Biocontrol Potential of <i>Trichoderma hamatum</i> Against Root Rot Pathogens |
| title_short | Unveiling the Genomic Features and Biocontrol Potential of <i>Trichoderma hamatum</i> Against Root Rot Pathogens |
| title_sort | unveiling the genomic features and biocontrol potential of i trichoderma hamatum i against root rot pathogens |
| topic | <i>T. hamatum</i> <i>Fusarium</i> biological control genome root rot disease |
| url | https://www.mdpi.com/2309-608X/11/2/126 |
| work_keys_str_mv | AT yuzhoufeng unveilingthegenomicfeaturesandbiocontrolpotentialofitrichodermahamatumiagainstrootrotpathogens AT xinyishuai unveilingthegenomicfeaturesandbiocontrolpotentialofitrichodermahamatumiagainstrootrotpathogens AT jilichen unveilingthegenomicfeaturesandbiocontrolpotentialofitrichodermahamatumiagainstrootrotpathogens AT qingzhang unveilingthegenomicfeaturesandbiocontrolpotentialofitrichodermahamatumiagainstrootrotpathogens AT lijiejia unveilingthegenomicfeaturesandbiocontrolpotentialofitrichodermahamatumiagainstrootrotpathogens AT luzhisun unveilingthegenomicfeaturesandbiocontrolpotentialofitrichodermahamatumiagainstrootrotpathogens AT yunxiasu unveilingthegenomicfeaturesandbiocontrolpotentialofitrichodermahamatumiagainstrootrotpathogens AT yanyansu unveilingthegenomicfeaturesandbiocontrolpotentialofitrichodermahamatumiagainstrootrotpathogens AT gangqiangdong unveilingthegenomicfeaturesandbiocontrolpotentialofitrichodermahamatumiagainstrootrotpathogens AT taoliu unveilingthegenomicfeaturesandbiocontrolpotentialofitrichodermahamatumiagainstrootrotpathogens AT guangqianglong unveilingthegenomicfeaturesandbiocontrolpotentialofitrichodermahamatumiagainstrootrotpathogens |