Unveiling the Genomic Features and Biocontrol Potential of <i>Trichoderma hamatum</i> Against Root Rot Pathogens

Unveiling the Genomic Features and Biocontrol Potential of <i>Trichoderma hamatum</i> Against Root Rot Pathogens

<i>Fusarium</i> species are among the most significant pathogens causing root rot in <i>Panax notoginseng</i>. In this study, a strain of <i>Trichoderma hamatum</i> was isolated from the rhizosphere soil of <i>P. notoginseng</i> and subjected to whole-...

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Bibliographic Details
Main Authors:	Yuzhou Feng, Xinyi Shuai, Jili Chen, Qing Zhang, Lijie Jia, Luzhi Sun, Yunxia Su, Yanyan Su, Gangqiang Dong, Tao Liu, Guangqiang Long
Format:	Article
Language:	English
Published:	MDPI AG 2025-02-01
Series:	Journal of Fungi
Subjects:	<i>T. hamatum</i> <i>Fusarium</i> biological control genome root rot disease
Online Access:	https://www.mdpi.com/2309-608X/11/2/126
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Summary:	<i>Fusarium</i> species are among the most significant pathogens causing root rot in <i>Panax notoginseng</i>. In this study, a strain of <i>Trichoderma hamatum</i> was isolated from the rhizosphere soil of <i>P. notoginseng</i> and subjected to whole-genome sequencing. Plate confrontation experiments were conducted to investigate the antagonistic effects of <i>T. hamatum</i> against <i>Fusarium oxysporum</i>, <i>Fusarium solani</i>, and <i>Fusarium acutatum</i>, the primary <i>Fusarium</i> species causing root rot. Whole-genome sequencing revealed 10,774 predicted genes in <i>T. hamatum</i>, of which 454 were associated with carbohydrate-active enzymes (CAZymes) involved in fungal cell wall degradation. Additionally, 11 biosynthetic gene clusters (BGCs) associated with antimicrobial production were identified, highlighting the biocontrol potential of <i>T. hamatum</i>. In plate confrontation experiments, <i>T. hamatum</i> showed substantial inhibition rates of 68.07%, 70.63%, and 66.12% against <i>F. oxysporum</i>, <i>F. solani</i>, and <i>F. acutatum</i>, respectively. Scanning electron microscopy suggested the hyperparasitism of <i>T. hamatum</i> against <i>F. solani</i>, which was characterized by spore production that adhered to the pathogen, thereby inhibiting its growth. These findings provide a theoretical foundation to enhance understanding of the biological control mechanisms of <i>T. hamatum</i>, supporting its potential applications in sustainable agriculture.
ISSN:	2309-608X