The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study

The objective of this paper is to describe in detail the method of organotypic longitudinal spinal cord slice culture and the scientific basis for its potential utility. The technique is based on the interface method, which was described previously and thereafter was modified in our laboratory. The...

Full description

Saved in:
Bibliographic Details
Main Authors: Joanna Sypecka, Sylwia Koniusz, Maria Kawalec, Anna Sarnowska
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2015/471216
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832545773861994496
author Joanna Sypecka
Sylwia Koniusz
Maria Kawalec
Anna Sarnowska
author_facet Joanna Sypecka
Sylwia Koniusz
Maria Kawalec
Anna Sarnowska
author_sort Joanna Sypecka
collection DOAJ
description The objective of this paper is to describe in detail the method of organotypic longitudinal spinal cord slice culture and the scientific basis for its potential utility. The technique is based on the interface method, which was described previously and thereafter was modified in our laboratory. The most important advantage of the presented model is the preservation of the intrinsic spinal cord fiber tract and the ventrodorsal polarity of the spinal cord. All the processes occurring during axonal growth, regeneration, synapse formation, and myelination could be visualized while being cultured in vitro for up to 4-5 weeks after the slices had been isolated. Both pups and adult animals can undergo the same, equally efficient procedures when going by the protocol in question. The urgent need for an appropriate in vitro model for spinal cord regeneration results from a greater number of clinical trials concerning regenerative medicine in the spinal cord injury and from still insufficient knowledge of the molecular mechanisms involved in the neuroreparative processes. The detailed method of organotypic longitudinal spinal cord slice culture is accompanied by examples of its application to studying biological processes to which both the CNS inhabiting and grafted cells are subjected.
format Article
id doaj-art-34607d26542c4da286a641c2a1233147
institution Kabale University
issn 1687-966X
1687-9678
language English
publishDate 2015-01-01
publisher Wiley
record_format Article
series Stem Cells International
spelling doaj-art-34607d26542c4da286a641c2a12331472025-02-03T07:24:46ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/471216471216The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell StudyJoanna Sypecka0Sylwia Koniusz1Maria Kawalec2Anna Sarnowska3NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, PolandNeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, PolandMolecular Biology Unit, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, PolandTranslative Platform for Regenerative Medicine, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, PolandThe objective of this paper is to describe in detail the method of organotypic longitudinal spinal cord slice culture and the scientific basis for its potential utility. The technique is based on the interface method, which was described previously and thereafter was modified in our laboratory. The most important advantage of the presented model is the preservation of the intrinsic spinal cord fiber tract and the ventrodorsal polarity of the spinal cord. All the processes occurring during axonal growth, regeneration, synapse formation, and myelination could be visualized while being cultured in vitro for up to 4-5 weeks after the slices had been isolated. Both pups and adult animals can undergo the same, equally efficient procedures when going by the protocol in question. The urgent need for an appropriate in vitro model for spinal cord regeneration results from a greater number of clinical trials concerning regenerative medicine in the spinal cord injury and from still insufficient knowledge of the molecular mechanisms involved in the neuroreparative processes. The detailed method of organotypic longitudinal spinal cord slice culture is accompanied by examples of its application to studying biological processes to which both the CNS inhabiting and grafted cells are subjected.http://dx.doi.org/10.1155/2015/471216
spellingShingle Joanna Sypecka
Sylwia Koniusz
Maria Kawalec
Anna Sarnowska
The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study
Stem Cells International
title The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study
title_full The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study
title_fullStr The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study
title_full_unstemmed The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study
title_short The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study
title_sort organotypic longitudinal spinal cord slice culture for stem cell study
url http://dx.doi.org/10.1155/2015/471216
work_keys_str_mv AT joannasypecka theorganotypiclongitudinalspinalcordslicecultureforstemcellstudy
AT sylwiakoniusz theorganotypiclongitudinalspinalcordslicecultureforstemcellstudy
AT mariakawalec theorganotypiclongitudinalspinalcordslicecultureforstemcellstudy
AT annasarnowska theorganotypiclongitudinalspinalcordslicecultureforstemcellstudy
AT joannasypecka organotypiclongitudinalspinalcordslicecultureforstemcellstudy
AT sylwiakoniusz organotypiclongitudinalspinalcordslicecultureforstemcellstudy
AT mariakawalec organotypiclongitudinalspinalcordslicecultureforstemcellstudy
AT annasarnowska organotypiclongitudinalspinalcordslicecultureforstemcellstudy