Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium.
The organ of Corti in the cochlea is a two-cell layered epithelium: one cell layer of mechanosensory hair cells that align into one row of inner and three rows of outer hair cells interdigitated with one cell layer of underlying supporting cells along the entire length of the cochlear spiral. These...
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Public Library of Science (PLoS)
2017-09-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006967&type=printable |
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| author | Ting Zhang Jinshu Xu Pascal Maire Pin-Xian Xu |
| author_facet | Ting Zhang Jinshu Xu Pascal Maire Pin-Xian Xu |
| author_sort | Ting Zhang |
| collection | DOAJ |
| description | The organ of Corti in the cochlea is a two-cell layered epithelium: one cell layer of mechanosensory hair cells that align into one row of inner and three rows of outer hair cells interdigitated with one cell layer of underlying supporting cells along the entire length of the cochlear spiral. These two types of epithelial cells are derived from common precursors in the four- to five-cell layered primordium and acquire functionally important shapes during terminal differentiation through the thinning process and convergent extension. Here, we have examined the role of Six1 in the establishment of the auditory sensory epithelium. Our data show that prior to terminal differentiation of the precursor cells, deletion of Six1 leads to formation of only a few hair cells and defective patterning of the sensory epithelium. Previous studies have suggested that downregulation of Sox2 expression in differentiating hair cells must occur after Atoh1 mRNA activation in order to allow Atoh1 protein accumulation due to antagonistic effects between Atoh1 and Sox2. Our analysis indicates that downregulation of Sox2 in the differentiating hair cells depends on Six1 activity. Furthermore, we found that Six1 is required for the maintenance of Fgf8 expression and dynamic distribution of N-cadherin and E-cadherin in the organ of Corti during differentiation. Together, our analyses uncover essential roles of Six1 in hair cell differentiation and formation of the organ of Corti in the mammalian cochlea. |
| format | Article |
| id | doaj-art-345e6aaff4e7482e8872ddbdee6b90c5 |
| institution | OA Journals |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-345e6aaff4e7482e8872ddbdee6b90c52025-08-20T02:03:47ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042017-09-01139e100696710.1371/journal.pgen.1006967Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium.Ting ZhangJinshu XuPascal MairePin-Xian XuThe organ of Corti in the cochlea is a two-cell layered epithelium: one cell layer of mechanosensory hair cells that align into one row of inner and three rows of outer hair cells interdigitated with one cell layer of underlying supporting cells along the entire length of the cochlear spiral. These two types of epithelial cells are derived from common precursors in the four- to five-cell layered primordium and acquire functionally important shapes during terminal differentiation through the thinning process and convergent extension. Here, we have examined the role of Six1 in the establishment of the auditory sensory epithelium. Our data show that prior to terminal differentiation of the precursor cells, deletion of Six1 leads to formation of only a few hair cells and defective patterning of the sensory epithelium. Previous studies have suggested that downregulation of Sox2 expression in differentiating hair cells must occur after Atoh1 mRNA activation in order to allow Atoh1 protein accumulation due to antagonistic effects between Atoh1 and Sox2. Our analysis indicates that downregulation of Sox2 in the differentiating hair cells depends on Six1 activity. Furthermore, we found that Six1 is required for the maintenance of Fgf8 expression and dynamic distribution of N-cadherin and E-cadherin in the organ of Corti during differentiation. Together, our analyses uncover essential roles of Six1 in hair cell differentiation and formation of the organ of Corti in the mammalian cochlea.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006967&type=printable |
| spellingShingle | Ting Zhang Jinshu Xu Pascal Maire Pin-Xian Xu Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium. PLoS Genetics |
| title | Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium. |
| title_full | Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium. |
| title_fullStr | Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium. |
| title_full_unstemmed | Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium. |
| title_short | Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium. |
| title_sort | six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006967&type=printable |
| work_keys_str_mv | AT tingzhang six1isessentialfordifferentiationandpatterningofthemammalianauditorysensoryepithelium AT jinshuxu six1isessentialfordifferentiationandpatterningofthemammalianauditorysensoryepithelium AT pascalmaire six1isessentialfordifferentiationandpatterningofthemammalianauditorysensoryepithelium AT pinxianxu six1isessentialfordifferentiationandpatterningofthemammalianauditorysensoryepithelium |