In Vitro Insulin Resistance Model: A Recent Update

Insulin resistance, which affects insulin-sensitive tissues, including adipose tissues, skeletal muscle, and the liver, is the central pathophysiological mechanism underlying type 2 diabetes progression. Decreased glucose uptake in insulin-sensitive tissues disrupts insulin signaling pathways, parti...

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Main Authors: Ratih D. Yudhani, Yulia Sari, Dwi A. A. Nugrahaningsih, Eti N. Sholikhah, Maftuchah Rochmanti, Abdul K. R. Purba, Husnul Khotimah, Dian Nugrahenny, Mustofa Mustofa
Format: Article
Language:English
Published: Wiley 2023-01-01
Series:Journal of Obesity
Online Access:http://dx.doi.org/10.1155/2023/1964732
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author Ratih D. Yudhani
Yulia Sari
Dwi A. A. Nugrahaningsih
Eti N. Sholikhah
Maftuchah Rochmanti
Abdul K. R. Purba
Husnul Khotimah
Dian Nugrahenny
Mustofa Mustofa
author_facet Ratih D. Yudhani
Yulia Sari
Dwi A. A. Nugrahaningsih
Eti N. Sholikhah
Maftuchah Rochmanti
Abdul K. R. Purba
Husnul Khotimah
Dian Nugrahenny
Mustofa Mustofa
author_sort Ratih D. Yudhani
collection DOAJ
description Insulin resistance, which affects insulin-sensitive tissues, including adipose tissues, skeletal muscle, and the liver, is the central pathophysiological mechanism underlying type 2 diabetes progression. Decreased glucose uptake in insulin-sensitive tissues disrupts insulin signaling pathways, particularly the PI3K/Akt pathway. An in vitro model is appropriate for studying the cellular and molecular mechanisms underlying insulin resistance because it is easy to maintain and the results can be easily reproduced. The application of cell-based models for exploring the pathogenesis of diabetes and insulin resistance as well as for developing drugs for these conditions is well known. However, a comprehensive review of in vitro insulin resistance models is lacking. Therefore, this review was conducted to provide a comprehensive overview and summary of the latest in vitro insulin resistance models, particularly 3T3-L1 (preadipocyte), C2C12 (skeletal muscle), and HepG2 (liver) cell lines induced with palmitic acid, high glucose, or chronic exposure to insulin.
format Article
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issn 2090-0716
language English
publishDate 2023-01-01
publisher Wiley
record_format Article
series Journal of Obesity
spelling doaj-art-345ba6b0775948638903f8b7916e735c2025-08-20T03:18:35ZengWileyJournal of Obesity2090-07162023-01-01202310.1155/2023/1964732In Vitro Insulin Resistance Model: A Recent UpdateRatih D. Yudhani0Yulia Sari1Dwi A. A. Nugrahaningsih2Eti N. Sholikhah3Maftuchah Rochmanti4Abdul K. R. Purba5Husnul Khotimah6Dian Nugrahenny7Mustofa Mustofa8Department of PharmacologyDepartment of ParasitologyDepartment of Pharmacology and TherapyDepartment of Pharmacology and TherapyDepartment of AnatomyDepartment of AnatomyDepartment of PharmacologyDepartment of PharmacologyDepartment of Pharmacology and TherapyInsulin resistance, which affects insulin-sensitive tissues, including adipose tissues, skeletal muscle, and the liver, is the central pathophysiological mechanism underlying type 2 diabetes progression. Decreased glucose uptake in insulin-sensitive tissues disrupts insulin signaling pathways, particularly the PI3K/Akt pathway. An in vitro model is appropriate for studying the cellular and molecular mechanisms underlying insulin resistance because it is easy to maintain and the results can be easily reproduced. The application of cell-based models for exploring the pathogenesis of diabetes and insulin resistance as well as for developing drugs for these conditions is well known. However, a comprehensive review of in vitro insulin resistance models is lacking. Therefore, this review was conducted to provide a comprehensive overview and summary of the latest in vitro insulin resistance models, particularly 3T3-L1 (preadipocyte), C2C12 (skeletal muscle), and HepG2 (liver) cell lines induced with palmitic acid, high glucose, or chronic exposure to insulin.http://dx.doi.org/10.1155/2023/1964732
spellingShingle Ratih D. Yudhani
Yulia Sari
Dwi A. A. Nugrahaningsih
Eti N. Sholikhah
Maftuchah Rochmanti
Abdul K. R. Purba
Husnul Khotimah
Dian Nugrahenny
Mustofa Mustofa
In Vitro Insulin Resistance Model: A Recent Update
Journal of Obesity
title In Vitro Insulin Resistance Model: A Recent Update
title_full In Vitro Insulin Resistance Model: A Recent Update
title_fullStr In Vitro Insulin Resistance Model: A Recent Update
title_full_unstemmed In Vitro Insulin Resistance Model: A Recent Update
title_short In Vitro Insulin Resistance Model: A Recent Update
title_sort in vitro insulin resistance model a recent update
url http://dx.doi.org/10.1155/2023/1964732
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AT yuliasari invitroinsulinresistancemodelarecentupdate
AT dwiaanugrahaningsih invitroinsulinresistancemodelarecentupdate
AT etinsholikhah invitroinsulinresistancemodelarecentupdate
AT maftuchahrochmanti invitroinsulinresistancemodelarecentupdate
AT abdulkrpurba invitroinsulinresistancemodelarecentupdate
AT husnulkhotimah invitroinsulinresistancemodelarecentupdate
AT diannugrahenny invitroinsulinresistancemodelarecentupdate
AT mustofamustofa invitroinsulinresistancemodelarecentupdate