Antibiogram and characterization of resistance markers among Escherichia coli Isolates from urinary tract infections

Introduction: Urinary tract infection is the most frequently diagnosed kidney and urologic disease, and Escherichia coli is by far its most common etiological agent. Uropathogenic E. coli are responsible for approximately 90% of urinary tract infections seen in individuals with ordinary anatomy the...

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Main Authors: Yashwant Kumar, Shivani Sood, Anshu Sharma, Kavaratty Raju Mani
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2013-07-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/2706
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author Yashwant Kumar
Shivani Sood
Anshu Sharma
Kavaratty Raju Mani
author_facet Yashwant Kumar
Shivani Sood
Anshu Sharma
Kavaratty Raju Mani
author_sort Yashwant Kumar
collection DOAJ
description Introduction: Urinary tract infection is the most frequently diagnosed kidney and urologic disease, and Escherichia coli is by far its most common etiological agent. Uropathogenic E. coli are responsible for approximately 90% of urinary tract infections seen in individuals with ordinary anatomy therefore, it is essential to review the antibiogram of uropathogenic E. coli periodically to help clinicians decide on the appropriate therapy. Methodology: We evaluated E. coli isolated from urinary tract infections at the National Salmonella and Escherichia Centre for antibiogram, plasmid transferability and stability of resistance markers. Results: In total, 90.9% of the isolates were found to be sensitive to nitrofurantoin while the highest proportion of the isolates was found to be resistant to nalidixic acid. Minimum inhibitory concentrations of all antimicrobials for different isolates were well within the limits specified by the Clinical and Laboratory Standards Institute.  Resistance against tetracycline was not transferred either by conjugation and transformation. Streptomycin resistance was found to be lost in the maximum number of tested isolates showing loss at the 10th, 15th and 20th passages. Conclusion: Changing trends in antibiotic resistance necessitates the periodic generation of antibiogram data to help health authorities revise treatment strategies for urinary tract infections caused by E. coli.
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spelling doaj-art-345a3c0a2d4a49aaa68d23347da58fd22025-08-20T02:27:23ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802013-07-0170710.3855/jidc.2706Antibiogram and characterization of resistance markers among Escherichia coli Isolates from urinary tract infectionsYashwant Kumar0Shivani Sood1Anshu Sharma2Kavaratty Raju Mani3National Salmonella and Escherichia Centre, Central Research Institute, Kasauli, Himachal Pradesh, IndiaNational Salmonella and Escherichia Centre, Central Research Institute, Kasauli, Himachal Pradesh, IndiaNational Salmonella and Escherichia Centre, Central Research Institute, Kasauli, Himachal Pradesh, IndiaNational Salmonella and Escherichia Centre, Central Research Institute, Kasauli, Himachal Pradesh, India Introduction: Urinary tract infection is the most frequently diagnosed kidney and urologic disease, and Escherichia coli is by far its most common etiological agent. Uropathogenic E. coli are responsible for approximately 90% of urinary tract infections seen in individuals with ordinary anatomy therefore, it is essential to review the antibiogram of uropathogenic E. coli periodically to help clinicians decide on the appropriate therapy. Methodology: We evaluated E. coli isolated from urinary tract infections at the National Salmonella and Escherichia Centre for antibiogram, plasmid transferability and stability of resistance markers. Results: In total, 90.9% of the isolates were found to be sensitive to nitrofurantoin while the highest proportion of the isolates was found to be resistant to nalidixic acid. Minimum inhibitory concentrations of all antimicrobials for different isolates were well within the limits specified by the Clinical and Laboratory Standards Institute.  Resistance against tetracycline was not transferred either by conjugation and transformation. Streptomycin resistance was found to be lost in the maximum number of tested isolates showing loss at the 10th, 15th and 20th passages. Conclusion: Changing trends in antibiotic resistance necessitates the periodic generation of antibiogram data to help health authorities revise treatment strategies for urinary tract infections caused by E. coli. https://jidc.org/index.php/journal/article/view/2706Escherichia coliurinary tract infectionsantibiogram
spellingShingle Yashwant Kumar
Shivani Sood
Anshu Sharma
Kavaratty Raju Mani
Antibiogram and characterization of resistance markers among Escherichia coli Isolates from urinary tract infections
Journal of Infection in Developing Countries
Escherichia coli
urinary tract infections
antibiogram
title Antibiogram and characterization of resistance markers among Escherichia coli Isolates from urinary tract infections
title_full Antibiogram and characterization of resistance markers among Escherichia coli Isolates from urinary tract infections
title_fullStr Antibiogram and characterization of resistance markers among Escherichia coli Isolates from urinary tract infections
title_full_unstemmed Antibiogram and characterization of resistance markers among Escherichia coli Isolates from urinary tract infections
title_short Antibiogram and characterization of resistance markers among Escherichia coli Isolates from urinary tract infections
title_sort antibiogram and characterization of resistance markers among escherichia coli isolates from urinary tract infections
topic Escherichia coli
urinary tract infections
antibiogram
url https://jidc.org/index.php/journal/article/view/2706
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