Effects of Vitamin C on the Prevention of Ischemia-Reperfusion Brain Injury: Experimental Study in Rats
Background. Reperfusion syndrome after carotid endarterectomy is a complication associated with cerebrovascular self-regulation in a chronically hypoperfused cerebral hemisphere, leading to severe neurological damage. Vitamin C is an important antioxidant in brain metabolism that has shown some neur...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Vascular Medicine |
| Online Access: | http://dx.doi.org/10.1155/2019/4090549 |
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| author | Kelston Paulo Felice de Sales Bruno Araújo Serra Pinto Nathalee Liberal Xavier Ribeiro Thamys Marinho Melo Leonardo Victor Galvão-Moreira Sebastião Barreto de Brito Filho Flávio Nigri |
| author_facet | Kelston Paulo Felice de Sales Bruno Araújo Serra Pinto Nathalee Liberal Xavier Ribeiro Thamys Marinho Melo Leonardo Victor Galvão-Moreira Sebastião Barreto de Brito Filho Flávio Nigri |
| author_sort | Kelston Paulo Felice de Sales |
| collection | DOAJ |
| description | Background. Reperfusion syndrome after carotid endarterectomy is a complication associated with cerebrovascular self-regulation in a chronically hypoperfused cerebral hemisphere, leading to severe neurological damage. Vitamin C is an important antioxidant in brain metabolism that has shown some neuroprotective actions. Objective. To investigate the potential effects of vitamin C on cerebral reperfusion in comparison with placebo (saline) in rats. Methods. Male Wistar rats were divided into 3 groups: (i) Sham (n=4), animals exposed to carotid arteries dissection without clamping; (ii) Control (n=4), animals that received an intraperitoneal injection of 0.9% saline solution (0.1 mL/kg) and underwent carotid arteries dissection with temporary clamping; (iii) Vitamin C (n=4), animals that received an intraperitoneal injection of vitamin C (750 mg/kg) and underwent carotid arteries dissection with temporary clamping. Behavioral assessment was then performed in all groups, which included the open field, Morris water maze and rotarod tests. Levels of malondialdehyde (MDA) in the hippocampus and striatum were measured using a fluorometric assay. Results. Rats treated with vitamin C presented with a similar behavior as compared to the Sham group in all the three tests (p>0.05), but it was significantly different from controls (p<0.05). Vitamin C was also found to reduce MDA levels in both hippocampus and striatum when compared to placebo (p<0.05). Conclusion. In the present study, vitamin C was associated with behavioral and motor preservation as well as decreased cerebral MDA levels after induced cerebral ischemia in rats. |
| format | Article |
| id | doaj-art-3456129ebfa74a929a1521a69ba5dda2 |
| institution | Kabale University |
| issn | 2090-2824 2090-2832 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Vascular Medicine |
| spelling | doaj-art-3456129ebfa74a929a1521a69ba5dda22025-08-20T03:55:06ZengWileyInternational Journal of Vascular Medicine2090-28242090-28322019-01-01201910.1155/2019/40905494090549Effects of Vitamin C on the Prevention of Ischemia-Reperfusion Brain Injury: Experimental Study in RatsKelston Paulo Felice de Sales0Bruno Araújo Serra Pinto1Nathalee Liberal Xavier Ribeiro2Thamys Marinho Melo3Leonardo Victor Galvão-Moreira4Sebastião Barreto de Brito Filho5Flávio Nigri6Department of Vascular and Endovascular Surgery, University Hospital, Federal University of Maranhão, São Luís, BrazilLaboratory of Experimental Physiology, Department of Physiological Sciences, Federal University of Maranhão, São Luís, BrazilLaboratory of Experimental Physiology, Department of Physiological Sciences, Federal University of Maranhão, São Luís, BrazilLaboratory of Experimental Physiology, Department of Physiological Sciences, Federal University of Maranhão, São Luís, BrazilSchool of Medicine, Federal University of Maranhão, São Luís, BrazilDepartment of Vascular and Endovascular Surgery, University Hospital, Federal University of Maranhão, São Luís, BrazilHead of Neurosurgery Teaching and Assistance Unit, Department of Surgical Specialties, Pedro Ernesto University Hospital, Nervous System Electric Stimulation Laboratory (LabEEL) – Neurosurgery Teaching and Assistance Unit, Pedro Ernesto University Hospital, Rio de Janeiro State University, Rio de Janeiro, BrazilBackground. Reperfusion syndrome after carotid endarterectomy is a complication associated with cerebrovascular self-regulation in a chronically hypoperfused cerebral hemisphere, leading to severe neurological damage. Vitamin C is an important antioxidant in brain metabolism that has shown some neuroprotective actions. Objective. To investigate the potential effects of vitamin C on cerebral reperfusion in comparison with placebo (saline) in rats. Methods. Male Wistar rats were divided into 3 groups: (i) Sham (n=4), animals exposed to carotid arteries dissection without clamping; (ii) Control (n=4), animals that received an intraperitoneal injection of 0.9% saline solution (0.1 mL/kg) and underwent carotid arteries dissection with temporary clamping; (iii) Vitamin C (n=4), animals that received an intraperitoneal injection of vitamin C (750 mg/kg) and underwent carotid arteries dissection with temporary clamping. Behavioral assessment was then performed in all groups, which included the open field, Morris water maze and rotarod tests. Levels of malondialdehyde (MDA) in the hippocampus and striatum were measured using a fluorometric assay. Results. Rats treated with vitamin C presented with a similar behavior as compared to the Sham group in all the three tests (p>0.05), but it was significantly different from controls (p<0.05). Vitamin C was also found to reduce MDA levels in both hippocampus and striatum when compared to placebo (p<0.05). Conclusion. In the present study, vitamin C was associated with behavioral and motor preservation as well as decreased cerebral MDA levels after induced cerebral ischemia in rats.http://dx.doi.org/10.1155/2019/4090549 |
| spellingShingle | Kelston Paulo Felice de Sales Bruno Araújo Serra Pinto Nathalee Liberal Xavier Ribeiro Thamys Marinho Melo Leonardo Victor Galvão-Moreira Sebastião Barreto de Brito Filho Flávio Nigri Effects of Vitamin C on the Prevention of Ischemia-Reperfusion Brain Injury: Experimental Study in Rats International Journal of Vascular Medicine |
| title | Effects of Vitamin C on the Prevention of Ischemia-Reperfusion Brain Injury: Experimental Study in Rats |
| title_full | Effects of Vitamin C on the Prevention of Ischemia-Reperfusion Brain Injury: Experimental Study in Rats |
| title_fullStr | Effects of Vitamin C on the Prevention of Ischemia-Reperfusion Brain Injury: Experimental Study in Rats |
| title_full_unstemmed | Effects of Vitamin C on the Prevention of Ischemia-Reperfusion Brain Injury: Experimental Study in Rats |
| title_short | Effects of Vitamin C on the Prevention of Ischemia-Reperfusion Brain Injury: Experimental Study in Rats |
| title_sort | effects of vitamin c on the prevention of ischemia reperfusion brain injury experimental study in rats |
| url | http://dx.doi.org/10.1155/2019/4090549 |
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