Impact of point‐of‐care maternal viral load testing at delivery on vertical HIV transmission risk assessment and neonatal prophylaxis: a cluster randomized trial

Abstract Introduction Despite global reductions in vertical HIV transmission (VHT), 120,000 children newly acquired HIV in 2023. High maternal viral load (VL) is a major risk factor for VHT. We estimated the impact of point‐of‐care (PoC) maternal VL testing at delivery in profiling the risk of VHT a...

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Main Authors: Anange Fred Lwilla, Kira Elsbernd, Siriel Boniface, Raphael Edom, Arlete Mahumane, Bindiya Meggi, W. Chris Buck, Joaquim Lequechane, Kassia Pereira, Nhamo Chiwerengo, Falume Chale, Chishamiso Mudenyanga, Dadirayi Mutsaka, Marianna Mueller, Nyanda E. Ntinginya, Nuno Taveira, Michael Hoelscher, Ilesh Jani, Arne Kroidl, Issa Sabi, and the LIFE Study Consortium
Format: Article
Language:English
Published: Wiley 2025-08-01
Series:Journal of the International AIDS Society
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Online Access:https://doi.org/10.1002/jia2.70021
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author Anange Fred Lwilla
Kira Elsbernd
Siriel Boniface
Raphael Edom
Arlete Mahumane
Bindiya Meggi
W. Chris Buck
Joaquim Lequechane
Kassia Pereira
Nhamo Chiwerengo
Falume Chale
Chishamiso Mudenyanga
Dadirayi Mutsaka
Marianna Mueller
Nyanda E. Ntinginya
Nuno Taveira
Michael Hoelscher
Ilesh Jani
Arne Kroidl
Issa Sabi
and the LIFE Study Consortium
author_facet Anange Fred Lwilla
Kira Elsbernd
Siriel Boniface
Raphael Edom
Arlete Mahumane
Bindiya Meggi
W. Chris Buck
Joaquim Lequechane
Kassia Pereira
Nhamo Chiwerengo
Falume Chale
Chishamiso Mudenyanga
Dadirayi Mutsaka
Marianna Mueller
Nyanda E. Ntinginya
Nuno Taveira
Michael Hoelscher
Ilesh Jani
Arne Kroidl
Issa Sabi
and the LIFE Study Consortium
author_sort Anange Fred Lwilla
collection DOAJ
description Abstract Introduction Despite global reductions in vertical HIV transmission (VHT), 120,000 children newly acquired HIV in 2023. High maternal viral load (VL) is a major risk factor for VHT. We estimated the impact of point‐of‐care (PoC) maternal VL testing at delivery in profiling the risk of VHT and its impact on appropriate postnatal prophylaxis for infants born to women living with HIV (WLWH). Methods The cluster‐randomized LIFE (Long term Impact on inFant hEalth) study was conducted at 28 health facilities in Tanzania and Mozambique from 2019 to 2021. At delivery, the intervention arm applied PoC maternal VL plus clinical criteria for VHT risk assessment, while the control arm used clinical criteria only. In Tanzania, both arms provided ePNP based on maternal risk factors, while Mozambique provided ePNP universally. We used mixed effects logistic regression to estimate the intervention effect on the proportion of infants at high risk (Tanzania and Mozambique) and infants at high risk receiving ePNP (Tanzania only). Results A total of 6467 WLWH were enrolled: 66.3% were diagnosed before the third trimester, 99% were on antiretroviral therapy and 78% were virally suppressed at delivery. Of 6564 newborns of WLWH included, 774 (11.7%) were identified to be at a high risk: 629 (19.3%) versus 145 (4.4%) in intervention and control arms, respectively; p<0.0001. In the intervention arm, 520 (82.7%) infants at high risk were classified only based on maternal PoC VL at delivery. In the control arm, 720 (21.8%) additional infants at high risk would have been identified if their mothers had received PoC VL assessment. In Tanzania, infants at high risk in the intervention arm were significantly more likely to receive ePNP: 59.5% versus 31.4% (OR 4.42, 95% CI: 1.09, 17.89). However, 40.5% from intervention arm and 68.6% from control arm did not receive ePNP despite high‐risk classification at delivery. Conclusions PoC maternal VL testing at delivery significantly increased the proportion of infants identified to be at high risk. Infants at high risk whose mothers received PoC VL at delivery were more often initiated on ePNP. However, the linkage of infants at high risk to appropriate prophylaxis remains suboptimal, warranting consideration of universal ePNP.
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spelling doaj-art-34492616bd4f4ac7b3d4cd31690f0d4c2025-08-25T18:26:38ZengWileyJournal of the International AIDS Society1758-26522025-08-01288n/an/a10.1002/jia2.70021Impact of point‐of‐care maternal viral load testing at delivery on vertical HIV transmission risk assessment and neonatal prophylaxis: a cluster randomized trialAnange Fred Lwilla0Kira Elsbernd1Siriel Boniface2Raphael Edom3Arlete Mahumane4Bindiya Meggi5W. Chris Buck6Joaquim Lequechane7Kassia Pereira8Nhamo Chiwerengo9Falume Chale10Chishamiso Mudenyanga11Dadirayi Mutsaka12Marianna Mueller13Nyanda E. Ntinginya14Nuno Taveira15Michael Hoelscher16Ilesh Jani17Arne Kroidl18Issa Sabi19and the LIFE Study ConsortiumMbeya Medical Research Center National Institute for Medical Research (NIMR) Mbeya TanzaniaInstitute of Infectious Diseases and Tropical Medicine LMU University Hospital LMU Munich Munich GermanyMbeya Medical Research Center National Institute for Medical Research (NIMR) Mbeya TanzaniaMbeya Medical Research Center National Institute for Medical Research (NIMR) Mbeya TanzaniaInstituto Nacional de Saúde (INS) Maputo MozambiqueInstituto Nacional de Saúde (INS) Maputo MozambiqueDavid Geffen School of Medicine University of California Los Angeles California USAInstituto Nacional de Saúde (INS) Maputo MozambiqueInstituto Nacional de Saúde (INS) Maputo MozambiqueMbeya Medical Research Center National Institute for Medical Research (NIMR) Mbeya TanzaniaInstituto Nacional de Saúde (INS) Maputo MozambiqueClinton Health Access Initiative (CHAI) Maputo MozambiqueClinton Health Access Initiative (CHAI) Maputo MozambiqueInstitute of Infectious Diseases and Tropical Medicine LMU University Hospital LMU Munich Munich GermanyMbeya Medical Research Center National Institute for Medical Research (NIMR) Mbeya TanzaniaInstituto Universitário Egas Moniz (IUEM) Lisbon PortugalInstitute of Infectious Diseases and Tropical Medicine LMU University Hospital LMU Munich Munich GermanyInstituto Nacional de Saúde (INS) Maputo MozambiqueInstitute of Infectious Diseases and Tropical Medicine LMU University Hospital LMU Munich Munich GermanyMbeya Medical Research Center National Institute for Medical Research (NIMR) Mbeya TanzaniaAbstract Introduction Despite global reductions in vertical HIV transmission (VHT), 120,000 children newly acquired HIV in 2023. High maternal viral load (VL) is a major risk factor for VHT. We estimated the impact of point‐of‐care (PoC) maternal VL testing at delivery in profiling the risk of VHT and its impact on appropriate postnatal prophylaxis for infants born to women living with HIV (WLWH). Methods The cluster‐randomized LIFE (Long term Impact on inFant hEalth) study was conducted at 28 health facilities in Tanzania and Mozambique from 2019 to 2021. At delivery, the intervention arm applied PoC maternal VL plus clinical criteria for VHT risk assessment, while the control arm used clinical criteria only. In Tanzania, both arms provided ePNP based on maternal risk factors, while Mozambique provided ePNP universally. We used mixed effects logistic regression to estimate the intervention effect on the proportion of infants at high risk (Tanzania and Mozambique) and infants at high risk receiving ePNP (Tanzania only). Results A total of 6467 WLWH were enrolled: 66.3% were diagnosed before the third trimester, 99% were on antiretroviral therapy and 78% were virally suppressed at delivery. Of 6564 newborns of WLWH included, 774 (11.7%) were identified to be at a high risk: 629 (19.3%) versus 145 (4.4%) in intervention and control arms, respectively; p<0.0001. In the intervention arm, 520 (82.7%) infants at high risk were classified only based on maternal PoC VL at delivery. In the control arm, 720 (21.8%) additional infants at high risk would have been identified if their mothers had received PoC VL assessment. In Tanzania, infants at high risk in the intervention arm were significantly more likely to receive ePNP: 59.5% versus 31.4% (OR 4.42, 95% CI: 1.09, 17.89). However, 40.5% from intervention arm and 68.6% from control arm did not receive ePNP despite high‐risk classification at delivery. Conclusions PoC maternal VL testing at delivery significantly increased the proportion of infants identified to be at high risk. Infants at high risk whose mothers received PoC VL at delivery were more often initiated on ePNP. However, the linkage of infants at high risk to appropriate prophylaxis remains suboptimal, warranting consideration of universal ePNP.https://doi.org/10.1002/jia2.70021HIV acquisitionsinfantnewbornpoint‐of‐care systemsrisk assessmentviral load
spellingShingle Anange Fred Lwilla
Kira Elsbernd
Siriel Boniface
Raphael Edom
Arlete Mahumane
Bindiya Meggi
W. Chris Buck
Joaquim Lequechane
Kassia Pereira
Nhamo Chiwerengo
Falume Chale
Chishamiso Mudenyanga
Dadirayi Mutsaka
Marianna Mueller
Nyanda E. Ntinginya
Nuno Taveira
Michael Hoelscher
Ilesh Jani
Arne Kroidl
Issa Sabi
and the LIFE Study Consortium
Impact of point‐of‐care maternal viral load testing at delivery on vertical HIV transmission risk assessment and neonatal prophylaxis: a cluster randomized trial
Journal of the International AIDS Society
HIV acquisitions
infant
newborn
point‐of‐care systems
risk assessment
viral load
title Impact of point‐of‐care maternal viral load testing at delivery on vertical HIV transmission risk assessment and neonatal prophylaxis: a cluster randomized trial
title_full Impact of point‐of‐care maternal viral load testing at delivery on vertical HIV transmission risk assessment and neonatal prophylaxis: a cluster randomized trial
title_fullStr Impact of point‐of‐care maternal viral load testing at delivery on vertical HIV transmission risk assessment and neonatal prophylaxis: a cluster randomized trial
title_full_unstemmed Impact of point‐of‐care maternal viral load testing at delivery on vertical HIV transmission risk assessment and neonatal prophylaxis: a cluster randomized trial
title_short Impact of point‐of‐care maternal viral load testing at delivery on vertical HIV transmission risk assessment and neonatal prophylaxis: a cluster randomized trial
title_sort impact of point of care maternal viral load testing at delivery on vertical hiv transmission risk assessment and neonatal prophylaxis a cluster randomized trial
topic HIV acquisitions
infant
newborn
point‐of‐care systems
risk assessment
viral load
url https://doi.org/10.1002/jia2.70021
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