Fertility-Enhancing Potential of P. amygdalas and J. regia Oil Mixture in Wistar Rats: Male/Female Infertility Models Assessment

Polyunsaturated fatty acids–rich nuts are a group of natural sources that have served as a tonic in the treatment of many ailments for centuries. In this regard, P. amygdalas and J. regia nuts are traditionally used in infertility treatments. However, so far, the main mechanisms for the fertility-en...

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Main Authors: Sadia Suri Kashif, Sadaf Naeem, Saira Saeed Khan, Shaheen Perveen, Nausheen Alam, Saba Zubair, Javeria Ameer
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Scientifica
Online Access:http://dx.doi.org/10.1155/sci5/3936227
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author Sadia Suri Kashif
Sadaf Naeem
Saira Saeed Khan
Shaheen Perveen
Nausheen Alam
Saba Zubair
Javeria Ameer
author_facet Sadia Suri Kashif
Sadaf Naeem
Saira Saeed Khan
Shaheen Perveen
Nausheen Alam
Saba Zubair
Javeria Ameer
author_sort Sadia Suri Kashif
collection DOAJ
description Polyunsaturated fatty acids–rich nuts are a group of natural sources that have served as a tonic in the treatment of many ailments for centuries. In this regard, P. amygdalas and J. regia nuts are traditionally used in infertility treatments. However, so far, the main mechanisms for the fertility-enhancing effects of these nuts in males/females are unknown. The present study was intended to evaluate the fertility-boosting effect of a mixture constituting P. amygdalas and J. regia oil on male/female infertility models and in two successive generations of rats; F0 (parents) and F1 (offspring). For the fertility assessment, male and female rats, 18 pairs (n = 36, 1:1, F0 generation), were separated into three groups and dosed with 2 and 4 mL/kg daily of oil mixture and saline, respectively, from precohabitation up to lactation. To determine the further protective role of the oil mixture in infertility, we designed ethanol-induced male and estradiol-induced female infertility models. Various parameters like hormonal, hematological, lipid profile, and antioxidant markers [superoxide dismutase (SOD), glutathione peroxidase (GPx)] were measured along with histopathology of sex organs. The continual exposure of F0 and F1 generations to the oil mixture did not affect the fertility index and survival index in females. However, in males, all sperm parameters were significantly improved in both generations. We have found pronounced fertility outcomes with oil mixture supplementation in both genders of F0 and F1 generations. Results showed that the oil mixture significantly restored (p<0.05) luteinizing hormone (LH), follicular stimulating hormone, GPx, plasma testosterone, and SOD activities in both models. Histological findings endorsed enhanced folliculogenesis and spermatogenesis with enhanced architecture. Our results strongly suggest that P. amygdalas and J. regia oil mixture might be a promising option in future for male/female sterility treatment. This finding could pave the way in infertile men and women for clinical trials. This is the first study that has provided an experimental rationale for a walnut and almond oil mixture combination as an effective candidate for fertility recovery by improving sex hormones and managing oxidative stress.
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spelling doaj-art-34327a2edba24bb2889846c552eaadea2025-08-20T02:14:30ZengWileyScientifica2090-908X2025-01-01202510.1155/sci5/3936227Fertility-Enhancing Potential of P. amygdalas and J. regia Oil Mixture in Wistar Rats: Male/Female Infertility Models AssessmentSadia Suri Kashif0Sadaf Naeem1Saira Saeed Khan2Shaheen Perveen3Nausheen Alam4Saba Zubair5Javeria Ameer6Faculty of Pharmacy and Pharmaceutical SciencesInstitute of Pharmaceutical SciencesFaculty of Pharmacy and Pharmaceutical SciencesInstitute of Pharmaceutical SciencesFaculty of PharmacyInstitute of Pharmaceutical SciencesFaculty of Pharmacy and Pharmaceutical SciencesPolyunsaturated fatty acids–rich nuts are a group of natural sources that have served as a tonic in the treatment of many ailments for centuries. In this regard, P. amygdalas and J. regia nuts are traditionally used in infertility treatments. However, so far, the main mechanisms for the fertility-enhancing effects of these nuts in males/females are unknown. The present study was intended to evaluate the fertility-boosting effect of a mixture constituting P. amygdalas and J. regia oil on male/female infertility models and in two successive generations of rats; F0 (parents) and F1 (offspring). For the fertility assessment, male and female rats, 18 pairs (n = 36, 1:1, F0 generation), were separated into three groups and dosed with 2 and 4 mL/kg daily of oil mixture and saline, respectively, from precohabitation up to lactation. To determine the further protective role of the oil mixture in infertility, we designed ethanol-induced male and estradiol-induced female infertility models. Various parameters like hormonal, hematological, lipid profile, and antioxidant markers [superoxide dismutase (SOD), glutathione peroxidase (GPx)] were measured along with histopathology of sex organs. The continual exposure of F0 and F1 generations to the oil mixture did not affect the fertility index and survival index in females. However, in males, all sperm parameters were significantly improved in both generations. We have found pronounced fertility outcomes with oil mixture supplementation in both genders of F0 and F1 generations. Results showed that the oil mixture significantly restored (p<0.05) luteinizing hormone (LH), follicular stimulating hormone, GPx, plasma testosterone, and SOD activities in both models. Histological findings endorsed enhanced folliculogenesis and spermatogenesis with enhanced architecture. Our results strongly suggest that P. amygdalas and J. regia oil mixture might be a promising option in future for male/female sterility treatment. This finding could pave the way in infertile men and women for clinical trials. This is the first study that has provided an experimental rationale for a walnut and almond oil mixture combination as an effective candidate for fertility recovery by improving sex hormones and managing oxidative stress.http://dx.doi.org/10.1155/sci5/3936227
spellingShingle Sadia Suri Kashif
Sadaf Naeem
Saira Saeed Khan
Shaheen Perveen
Nausheen Alam
Saba Zubair
Javeria Ameer
Fertility-Enhancing Potential of P. amygdalas and J. regia Oil Mixture in Wistar Rats: Male/Female Infertility Models Assessment
Scientifica
title Fertility-Enhancing Potential of P. amygdalas and J. regia Oil Mixture in Wistar Rats: Male/Female Infertility Models Assessment
title_full Fertility-Enhancing Potential of P. amygdalas and J. regia Oil Mixture in Wistar Rats: Male/Female Infertility Models Assessment
title_fullStr Fertility-Enhancing Potential of P. amygdalas and J. regia Oil Mixture in Wistar Rats: Male/Female Infertility Models Assessment
title_full_unstemmed Fertility-Enhancing Potential of P. amygdalas and J. regia Oil Mixture in Wistar Rats: Male/Female Infertility Models Assessment
title_short Fertility-Enhancing Potential of P. amygdalas and J. regia Oil Mixture in Wistar Rats: Male/Female Infertility Models Assessment
title_sort fertility enhancing potential of p amygdalas and j regia oil mixture in wistar rats male female infertility models assessment
url http://dx.doi.org/10.1155/sci5/3936227
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