Accumulation differences of high-value ingredients in different phenotype Lonicera macranthoides: insights from integrative metabolome and transcriptome analyses
BackgroundLonicera macranthoides Hand.-Mazz., the primary sources of Lonicerae Flos(Shanyinhua), brings great medicinal and economic value as an invaluable source of natural bioactive compounds. Nutrient and metabolites accumulation generally changed accompany with its floral development and opening...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1533263/full |
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| author | Juan Zeng Juan Zeng Yu Qing Long Yu Qing Long Jia Yuan Zhu Jia Yuan Zhu Xue Sen Fu Xue Sen Fu Jing Yu Zhang Jing Yu Zhang Jia Wei He Jia Wei He Xiao Rong Liu Xiao Rong Liu Zhi Hui Wang Zhi Hui Wang Qiao Zhen Tong Qiao Zhen Tong Xiang Dan Liu Xiang Dan Liu Xiang Dan Liu Ri Bao Zhou Ri Bao Zhou Ri Bao Zhou |
| author_facet | Juan Zeng Juan Zeng Yu Qing Long Yu Qing Long Jia Yuan Zhu Jia Yuan Zhu Xue Sen Fu Xue Sen Fu Jing Yu Zhang Jing Yu Zhang Jia Wei He Jia Wei He Xiao Rong Liu Xiao Rong Liu Zhi Hui Wang Zhi Hui Wang Qiao Zhen Tong Qiao Zhen Tong Xiang Dan Liu Xiang Dan Liu Xiang Dan Liu Ri Bao Zhou Ri Bao Zhou Ri Bao Zhou |
| author_sort | Juan Zeng |
| collection | DOAJ |
| description | BackgroundLonicera macranthoides Hand.-Mazz., the primary sources of Lonicerae Flos(Shanyinhua), brings great medicinal and economic value as an invaluable source of natural bioactive compounds. Nutrient and metabolites accumulation generally changed accompany with its floral development and opening. While the specific accumulation pattern and the underlying molecular regulatory networks remain unclear. MethodsThe present study intergrated a comparative analysis upon UPLC-MS/MS-based metabolomics and RNA-seq-based transcriptomics to revealed the differences in accumulation of flavonoids, phenolic acids, and terpenoids between the xianglei-type (corolla-closed) and wild-type (corolla-unfolded) of L. macranthoides flowers. Results and conclusion674 differentially accumulated metabolites(DAMs) were identified in WT and XL, with 5,776 common differentially expressed genes(DEGs), revealing a significant differences in accumulation of flavonoids, phenolic acids, and terpenoids during the late stage of flower development between the xianglei-type and wild-type of L. macranthoides flowers. Combined analysis further identified 36 hub genes, major transcription factors and hormone-related genes, which play key roles in the differential accumulation of the abovementioned metabolites. These lines of evidences provide a molecular basis for the metabolic changes occurring during growth and can be significantly implicated in further research on the biosynthetic pathways associated with high-value potent active components in woody plants. |
| format | Article |
| id | doaj-art-342ef50e3e2a429383795cc1c7661994 |
| institution | DOAJ |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-342ef50e3e2a429383795cc1c76619942025-08-20T02:46:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-03-011610.3389/fpls.2025.15332631533263Accumulation differences of high-value ingredients in different phenotype Lonicera macranthoides: insights from integrative metabolome and transcriptome analysesJuan Zeng0Juan Zeng1Yu Qing Long2Yu Qing Long3Jia Yuan Zhu4Jia Yuan Zhu5Xue Sen Fu6Xue Sen Fu7Jing Yu Zhang8Jing Yu Zhang9Jia Wei He10Jia Wei He11Xiao Rong Liu12Xiao Rong Liu13Zhi Hui Wang14Zhi Hui Wang15Qiao Zhen Tong16Qiao Zhen Tong17Xiang Dan Liu18Xiang Dan Liu19Xiang Dan Liu20Ri Bao Zhou21Ri Bao Zhou22Ri Bao Zhou23School of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaKey Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaDepartment of Pharmacy, Yiyang Medical College, Yiyang, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaKey Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaKey Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaKey Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaKey Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaKey Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaKey Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaKey Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaKey Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, ChinaKey Laboratory of Modern Research of TCM, Education Department of Hunan Province, Changsha, ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, ChinaKey Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, ChinaKey Laboratory of Modern Research of TCM, Education Department of Hunan Province, Changsha, ChinaBackgroundLonicera macranthoides Hand.-Mazz., the primary sources of Lonicerae Flos(Shanyinhua), brings great medicinal and economic value as an invaluable source of natural bioactive compounds. Nutrient and metabolites accumulation generally changed accompany with its floral development and opening. While the specific accumulation pattern and the underlying molecular regulatory networks remain unclear. MethodsThe present study intergrated a comparative analysis upon UPLC-MS/MS-based metabolomics and RNA-seq-based transcriptomics to revealed the differences in accumulation of flavonoids, phenolic acids, and terpenoids between the xianglei-type (corolla-closed) and wild-type (corolla-unfolded) of L. macranthoides flowers. Results and conclusion674 differentially accumulated metabolites(DAMs) were identified in WT and XL, with 5,776 common differentially expressed genes(DEGs), revealing a significant differences in accumulation of flavonoids, phenolic acids, and terpenoids during the late stage of flower development between the xianglei-type and wild-type of L. macranthoides flowers. Combined analysis further identified 36 hub genes, major transcription factors and hormone-related genes, which play key roles in the differential accumulation of the abovementioned metabolites. These lines of evidences provide a molecular basis for the metabolic changes occurring during growth and can be significantly implicated in further research on the biosynthetic pathways associated with high-value potent active components in woody plants. https://www.frontiersin.org/articles/10.3389/fpls.2025.1533263/fullLonicera macranthoides Hand.-Mazz.metabolometranscriptomeactive ingredientsregulatory network |
| spellingShingle | Juan Zeng Juan Zeng Yu Qing Long Yu Qing Long Jia Yuan Zhu Jia Yuan Zhu Xue Sen Fu Xue Sen Fu Jing Yu Zhang Jing Yu Zhang Jia Wei He Jia Wei He Xiao Rong Liu Xiao Rong Liu Zhi Hui Wang Zhi Hui Wang Qiao Zhen Tong Qiao Zhen Tong Xiang Dan Liu Xiang Dan Liu Xiang Dan Liu Ri Bao Zhou Ri Bao Zhou Ri Bao Zhou Accumulation differences of high-value ingredients in different phenotype Lonicera macranthoides: insights from integrative metabolome and transcriptome analyses Frontiers in Plant Science Lonicera macranthoides Hand.-Mazz. metabolome transcriptome active ingredients regulatory network |
| title | Accumulation differences of high-value ingredients in different phenotype Lonicera macranthoides: insights from integrative metabolome and transcriptome analyses |
| title_full | Accumulation differences of high-value ingredients in different phenotype Lonicera macranthoides: insights from integrative metabolome and transcriptome analyses |
| title_fullStr | Accumulation differences of high-value ingredients in different phenotype Lonicera macranthoides: insights from integrative metabolome and transcriptome analyses |
| title_full_unstemmed | Accumulation differences of high-value ingredients in different phenotype Lonicera macranthoides: insights from integrative metabolome and transcriptome analyses |
| title_short | Accumulation differences of high-value ingredients in different phenotype Lonicera macranthoides: insights from integrative metabolome and transcriptome analyses |
| title_sort | accumulation differences of high value ingredients in different phenotype lonicera macranthoides insights from integrative metabolome and transcriptome analyses |
| topic | Lonicera macranthoides Hand.-Mazz. metabolome transcriptome active ingredients regulatory network |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1533263/full |
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