Antibiotic resistance genes in the animal manure-amended soil-plant system: Occurrence, transmission, bacterial hosts, and human health risks

Animal manure is a hotspot of antibiotic resistance genes (ARGs). Manure fertilization, a globally prevalent agricultural practice, poses potential risks to food safety and human health by serving as a significant pathway for resistomes to enter the soil–plant system. In this review, the occurrence...

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Main Authors: Bingshen Liu, Zenan Shen, Qian Zhou, Jiayun Ren, Haiqing Wang, Daoyong Zhang, Xiangliang Pan
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Ecotoxicology and Environmental Safety
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Online Access:http://www.sciencedirect.com/science/article/pii/S0147651325012357
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author Bingshen Liu
Zenan Shen
Qian Zhou
Jiayun Ren
Haiqing Wang
Daoyong Zhang
Xiangliang Pan
author_facet Bingshen Liu
Zenan Shen
Qian Zhou
Jiayun Ren
Haiqing Wang
Daoyong Zhang
Xiangliang Pan
author_sort Bingshen Liu
collection DOAJ
description Animal manure is a hotspot of antibiotic resistance genes (ARGs). Manure fertilization, a globally prevalent agricultural practice, poses potential risks to food safety and human health by serving as a significant pathway for resistomes to enter the soil–plant system. In this review, the occurrence and variability of ARGs in animal manure are introduced, and the impacts of manure application on ARG profiles in soils are assessed. Furthermore, a detailed summary is provided on the transmission pathways of resistomes from manure-amended soils to different plant compartments, including the endosphere and phyllosphere. Considering the critical role of ARG hosts in human health risk assessment, we provide a comprehensive summary of their taxonomic diversity as detected through culture–independent approaches. In addition, the research progress on human exposure risks associated with ARGs is discussed, including exposure pathways and indicators for risk assessment. Finally, this review outlines future research directions, including the development of mitigation strategies (focusing on attenuation mechanisms and transmission pathways of ARGs), the quantification of contributions from various ARG transmission pathways, and the systematic identification of ARG hosts. This review familiarizes readers with the current research status of ARGs in the manure-amended soil-plant system, highlighting the human exposure risks associated with ARG transmission across the manure-soil-plant continuum.
format Article
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institution Kabale University
issn 0147-6513
language English
publishDate 2025-09-01
publisher Elsevier
record_format Article
series Ecotoxicology and Environmental Safety
spelling doaj-art-342a9290a6714812a325d0725a061df22025-08-20T03:47:06ZengElsevierEcotoxicology and Environmental Safety0147-65132025-09-0130311889010.1016/j.ecoenv.2025.118890Antibiotic resistance genes in the animal manure-amended soil-plant system: Occurrence, transmission, bacterial hosts, and human health risksBingshen Liu0Zenan Shen1Qian Zhou2Jiayun Ren3Haiqing Wang4Daoyong Zhang5Xiangliang Pan6Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Shandong University of Aeronautics, Binzhou, Shandong 256603, China; Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China; Correspondence to: Shandong University of Aeronautics, Binzhou, Shandong 256603, China.Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, ChinaKey Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, ChinaShandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Shandong University of Aeronautics, Binzhou, Shandong 256603, ChinaShandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Shandong University of Aeronautics, Binzhou, Shandong 256603, ChinaKey Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, ChinaKey Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China; Corresponding author.Animal manure is a hotspot of antibiotic resistance genes (ARGs). Manure fertilization, a globally prevalent agricultural practice, poses potential risks to food safety and human health by serving as a significant pathway for resistomes to enter the soil–plant system. In this review, the occurrence and variability of ARGs in animal manure are introduced, and the impacts of manure application on ARG profiles in soils are assessed. Furthermore, a detailed summary is provided on the transmission pathways of resistomes from manure-amended soils to different plant compartments, including the endosphere and phyllosphere. Considering the critical role of ARG hosts in human health risk assessment, we provide a comprehensive summary of their taxonomic diversity as detected through culture–independent approaches. In addition, the research progress on human exposure risks associated with ARGs is discussed, including exposure pathways and indicators for risk assessment. Finally, this review outlines future research directions, including the development of mitigation strategies (focusing on attenuation mechanisms and transmission pathways of ARGs), the quantification of contributions from various ARG transmission pathways, and the systematic identification of ARG hosts. This review familiarizes readers with the current research status of ARGs in the manure-amended soil-plant system, highlighting the human exposure risks associated with ARG transmission across the manure-soil-plant continuum.http://www.sciencedirect.com/science/article/pii/S0147651325012357Antibiotic resistance genesManure fertilizationPlant endosphereTransmission pathwaysHost identification
spellingShingle Bingshen Liu
Zenan Shen
Qian Zhou
Jiayun Ren
Haiqing Wang
Daoyong Zhang
Xiangliang Pan
Antibiotic resistance genes in the animal manure-amended soil-plant system: Occurrence, transmission, bacterial hosts, and human health risks
Ecotoxicology and Environmental Safety
Antibiotic resistance genes
Manure fertilization
Plant endosphere
Transmission pathways
Host identification
title Antibiotic resistance genes in the animal manure-amended soil-plant system: Occurrence, transmission, bacterial hosts, and human health risks
title_full Antibiotic resistance genes in the animal manure-amended soil-plant system: Occurrence, transmission, bacterial hosts, and human health risks
title_fullStr Antibiotic resistance genes in the animal manure-amended soil-plant system: Occurrence, transmission, bacterial hosts, and human health risks
title_full_unstemmed Antibiotic resistance genes in the animal manure-amended soil-plant system: Occurrence, transmission, bacterial hosts, and human health risks
title_short Antibiotic resistance genes in the animal manure-amended soil-plant system: Occurrence, transmission, bacterial hosts, and human health risks
title_sort antibiotic resistance genes in the animal manure amended soil plant system occurrence transmission bacterial hosts and human health risks
topic Antibiotic resistance genes
Manure fertilization
Plant endosphere
Transmission pathways
Host identification
url http://www.sciencedirect.com/science/article/pii/S0147651325012357
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