Molecular Identification and Monitoring of Delia platura (Meigen) (Diptera: Anthomyiidae) Infesting Cucumber Plants in Dhi Qar Province
Delia platura species is a small pest causes enormous damage and losses in a wide range of crops. An identification study was conducted through molecular characterization and sequencing to distinguish the species of Delia genus, which attack cucumber seedlings in greenhouses. The study was carried o...
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University of Basrah
2025-08-01
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| Series: | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ |
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| Online Access: | https://bjas.bajas.edu.iq/index.php/bjas/article/view/2652 |
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| author | Mustafa J. ALFayyadh Sindab S. J. AL-Dahwi |
| author_facet | Mustafa J. ALFayyadh Sindab S. J. AL-Dahwi |
| author_sort | Mustafa J. ALFayyadh |
| collection | DOAJ |
| description | Delia platura species is a small pest causes enormous damage and losses in a wide range of crops. An identification study was conducted through molecular characterization and sequencing to distinguish the species of Delia genus, which attack cucumber seedlings in greenhouses. The study was carried out in Al-Gharraf region in Dhi Qar province, Southern Iraq, during the spring and Autumn seasons. In the present study, mtCOI was amplified for molecular identification approaches to confirm the morphology characterization. Two collected specimens were sequenced using the Sanger approach. The BioEdit and MEGA software were used for phylogenetic analysis. The results of pairwise sequence and genetic analysis showed that there was 99.26% match with the Delia platura species registered in the database GenBank. The identified species with accession number PV114986.1 showed high similarity with species isolated from the Chinese (KP901268.1) and Norway (MG673907.1). The result of traps reported that the yellow-colored traps recorded 2.13 adults/trap during 15 days. This trap was significantly more attractive than other colored traps (white, green, and red). However, white colored traps attracted 0.60 adults/trap with the same period above, while the green and red colored traps did not catch any insects.
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| format | Article |
| id | doaj-art-3426152c74994b8e8871fef2bcd0f049 |
| institution | Kabale University |
| issn | 1814-5868 2520-0860 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | University of Basrah |
| record_format | Article |
| series | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ |
| spelling | doaj-art-3426152c74994b8e8871fef2bcd0f0492025-08-25T13:55:52ZengUniversity of BasrahMaǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ1814-58682520-08602025-08-0138(Special Issue)Molecular Identification and Monitoring of Delia platura (Meigen) (Diptera: Anthomyiidae) Infesting Cucumber Plants in Dhi Qar ProvinceMustafa J. ALFayyadh0Sindab S. J. AL-Dahwi1Department of Soil Sciences and Water Resources / College of Agriculture - Sumer University / Dhi Qar – IraqDepartment of Plant Protection, College of Agricultural Engineering Science, University of Baghdad, Baghdad, IraqDelia platura species is a small pest causes enormous damage and losses in a wide range of crops. An identification study was conducted through molecular characterization and sequencing to distinguish the species of Delia genus, which attack cucumber seedlings in greenhouses. The study was carried out in Al-Gharraf region in Dhi Qar province, Southern Iraq, during the spring and Autumn seasons. In the present study, mtCOI was amplified for molecular identification approaches to confirm the morphology characterization. Two collected specimens were sequenced using the Sanger approach. The BioEdit and MEGA software were used for phylogenetic analysis. The results of pairwise sequence and genetic analysis showed that there was 99.26% match with the Delia platura species registered in the database GenBank. The identified species with accession number PV114986.1 showed high similarity with species isolated from the Chinese (KP901268.1) and Norway (MG673907.1). The result of traps reported that the yellow-colored traps recorded 2.13 adults/trap during 15 days. This trap was significantly more attractive than other colored traps (white, green, and red). However, white colored traps attracted 0.60 adults/trap with the same period above, while the green and red colored traps did not catch any insects. https://bjas.bajas.edu.iq/index.php/bjas/article/view/2652COI geneCucumberDelia platuraMolecular Identificationcolors traps |
| spellingShingle | Mustafa J. ALFayyadh Sindab S. J. AL-Dahwi Molecular Identification and Monitoring of Delia platura (Meigen) (Diptera: Anthomyiidae) Infesting Cucumber Plants in Dhi Qar Province Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ COI gene Cucumber Delia platura Molecular Identification colors traps |
| title | Molecular Identification and Monitoring of Delia platura (Meigen) (Diptera: Anthomyiidae) Infesting Cucumber Plants in Dhi Qar Province |
| title_full | Molecular Identification and Monitoring of Delia platura (Meigen) (Diptera: Anthomyiidae) Infesting Cucumber Plants in Dhi Qar Province |
| title_fullStr | Molecular Identification and Monitoring of Delia platura (Meigen) (Diptera: Anthomyiidae) Infesting Cucumber Plants in Dhi Qar Province |
| title_full_unstemmed | Molecular Identification and Monitoring of Delia platura (Meigen) (Diptera: Anthomyiidae) Infesting Cucumber Plants in Dhi Qar Province |
| title_short | Molecular Identification and Monitoring of Delia platura (Meigen) (Diptera: Anthomyiidae) Infesting Cucumber Plants in Dhi Qar Province |
| title_sort | molecular identification and monitoring of delia platura meigen diptera anthomyiidae infesting cucumber plants in dhi qar province |
| topic | COI gene Cucumber Delia platura Molecular Identification colors traps |
| url | https://bjas.bajas.edu.iq/index.php/bjas/article/view/2652 |
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