Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite
Sensitive detection of Staphylococcus aureus enterotoxin B (SEB) is of importance for preventing food poisoning from threatening human health. In this work, an electrochemical and colorimetric dual-signal detection assay of SEB was developed. The probe (Ab2/AuPt@Fe-N-C) was bound to SEB captured by...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250168 |
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| author | Huan Liang Hongcheng Liu Haojian Lin Guobao Ning Xiaokang Lu Siying Ma Fei Liu Hui Zhao Canpeng Li |
| author_facet | Huan Liang Hongcheng Liu Haojian Lin Guobao Ning Xiaokang Lu Siying Ma Fei Liu Hui Zhao Canpeng Li |
| author_sort | Huan Liang |
| collection | DOAJ |
| description | Sensitive detection of Staphylococcus aureus enterotoxin B (SEB) is of importance for preventing food poisoning from threatening human health. In this work, an electrochemical and colorimetric dual-signal detection assay of SEB was developed. The probe (Ab2/AuPt@Fe-N-C) was bound to SEB captured by Ab1, where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal. Furthermore, the probe catalyzed the oxidation of 3, 3’, 5, 5’-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm. Once the target was captured and formed a sandwich-like complex, the color changed from colorless to blue. SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.0002–10.0000 and 0.0005–10.0000 ng/mL, with limits of detection of 0.0667 and 0.1670 pg/mL, respectively. The dual-signal biosensor was successfully used to detect SEB in milk and water samples, which has great potential in toxin detection in food and the environment. |
| format | Article |
| id | doaj-art-341957441f484b69ac7d3e66623cb10b |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-341957441f484b69ac7d3e66623cb10b2025-01-10T06:56:48ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011342025203510.26599/FSHW.2022.9250168Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocompositeHuan Liang0Hongcheng Liu1Haojian Lin2Guobao Ning3Xiaokang Lu4Siying Ma5Fei Liu6Hui Zhao7Canpeng Li8School of Chemical Science and Technology, Yunnan University, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Kunming 650091, ChinaInstitute of Quality Standard and Testing Technology, Yunnan Academy of Agricultural Science, Supervision and Testing Center for Farm Product Quality, Ministry of Agriculture, Kunming 650223, ChinaState Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou 510275, ChinaSchool of Chemical Science and Technology, Yunnan University, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Kunming 650091, ChinaSchool of Chemical Science and Technology, Yunnan University, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Kunming 650091, ChinaLaboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming 650091, ChinaState Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou 510275, ChinaLaboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming 650091, ChinaSchool of Chemical Science and Technology, Yunnan University, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Kunming 650091, ChinaSensitive detection of Staphylococcus aureus enterotoxin B (SEB) is of importance for preventing food poisoning from threatening human health. In this work, an electrochemical and colorimetric dual-signal detection assay of SEB was developed. The probe (Ab2/AuPt@Fe-N-C) was bound to SEB captured by Ab1, where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal. Furthermore, the probe catalyzed the oxidation of 3, 3’, 5, 5’-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm. Once the target was captured and formed a sandwich-like complex, the color changed from colorless to blue. SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.0002–10.0000 and 0.0005–10.0000 ng/mL, with limits of detection of 0.0667 and 0.1670 pg/mL, respectively. The dual-signal biosensor was successfully used to detect SEB in milk and water samples, which has great potential in toxin detection in food and the environment.https://www.sciopen.com/article/10.26599/FSHW.2022.9250168enterotoxinelectrochemical immunosensorcolorimetric assaymof@borophene compositedual-functional fe-n-c signal atom catalyst |
| spellingShingle | Huan Liang Hongcheng Liu Haojian Lin Guobao Ning Xiaokang Lu Siying Ma Fei Liu Hui Zhao Canpeng Li Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite Food Science and Human Wellness enterotoxin electrochemical immunosensor colorimetric assay mof@borophene composite dual-functional fe-n-c signal atom catalyst |
| title | Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite |
| title_full | Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite |
| title_fullStr | Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite |
| title_full_unstemmed | Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite |
| title_short | Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite |
| title_sort | electrochemical and colorimetric dual signal detection of staphylococcus aureus enterotoxin b based on aupt bimetallic nanoparticles loaded fe n c single atom nanocomposite |
| topic | enterotoxin electrochemical immunosensor colorimetric assay mof@borophene composite dual-functional fe-n-c signal atom catalyst |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250168 |
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