Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil
Introduction: Serratia marcescens is an opportunistic pathogen found ubiquitously in the environment and associated with a wide range of nosocomial infections. This multidrug-resistant bacterium has been a cause of concern for hospitals and healthcare facilities due to its ability to spread rapidly...
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The Journal of Infection in Developing Countries
2024-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/18913 |
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| author | Thalita PPS Cruz Francisco KSF Azevedo Marco A Pepato Paula S Rizzo Francisco JD Souto Bertram Brenig Aristóteles Góes-Neto Siomar C Soares Rommel TJ Ramos Vasco AC Azevedo Mateus M Costa Leonardo Broetto Luciano Nakazato Valéria Dutra |
| author_facet | Thalita PPS Cruz Francisco KSF Azevedo Marco A Pepato Paula S Rizzo Francisco JD Souto Bertram Brenig Aristóteles Góes-Neto Siomar C Soares Rommel TJ Ramos Vasco AC Azevedo Mateus M Costa Leonardo Broetto Luciano Nakazato Valéria Dutra |
| author_sort | Thalita PPS Cruz |
| collection | DOAJ |
| description |
Introduction: Serratia marcescens is an opportunistic pathogen found ubiquitously in the environment and associated with a wide range of nosocomial infections. This multidrug-resistant bacterium has been a cause of concern for hospitals and healthcare facilities due to its ability to spread rapidly and cause outbreaks. Next generation sequencing genotyping of bacterial isolates has proven to be a valuable tool for tracking the spread and transmission of nosocomial infections. This has allowed for the identification of outbreaks and transmission chains, as well as determining whether cases are due to endogenous or exogenous sources. Evidence of nosocomial transmission has been gathered through genotyping methods. The aim of this study was to investigate the genetic diversity of carbapenemase-producing S. marcescens in an outbreak at a public hospital in Cuiaba, MT, Brazil.
Methodology: Ten isolates of S. marcenses were sequenced and antibiotic resistance profiles analyzed over 12 days.
Results: The isolates were clonal and multidrug resistant. Gentamycin and tigecycline had sensitivity in 90% and 80% isolates, respectively. Genomic analysis identified several genes that encode β-lactamases, aminoglycoside-modifying enzymes, efflux pumps, and other virulence factors.
Conclusions: Systematic surveillance is crucial in monitoring the evolution of S. marcescens genotypes, as it can lead to early detection and prevention of outbreaks.
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| format | Article |
| id | doaj-art-340e3ca16b5a40debc6e581a24309e00 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-340e3ca16b5a40debc6e581a24309e002025-08-20T02:57:03ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-05-01180510.3855/jidc.18913Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in BrazilThalita PPS Cruz0Francisco KSF Azevedo1Marco A Pepato2Paula S Rizzo3Francisco JD Souto4Bertram Brenig5Aristóteles Góes-Neto6Siomar C Soares7Rommel TJ Ramos8Vasco AC Azevedo9Mateus M Costa10Leonardo Broetto11Luciano Nakazato12Valéria Dutra13Laboratory of Veterinary Microbiology and Molecular Biology, Veterinary Hospital of Federal University of Mato Grosso, Cuiabá, MT, BrazilDepartment of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, BrazilDepartment of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, BrazilDepartment of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, BrazilDepartment of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, BrazilInstitute of Veterinary Medicine, University of Göttingen, Göttingen, Niedersachsen, GermanyLaboratory of Molecular Computational Biology of Fungi (LBMCF), Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Microbiology, Immunology, and Parasitology, Federal University of Triangle Miner, Uberaba, BrazilCenter of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Pará, BrazilLaboratory of Cellular and Molecular Genetics (LGCM), Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, BrazilLaboratory of Microbiology and Animal Immunology, Federal University of Vale of São Francisco (Univasf), Petrolina, Pernambuco, Brazil - Center for Open Access Genomic Analysis (CALAnGO), Federal University of Vale of São Francisco (Univasf), Petrolina, Pernambuco, BrazilMulticentric Graduate Program in Biochemistry and Molecular Biology at SBBq (PMBqBM), Federal University of Alagoas, BrazilLaboratory of Veterinary Microbiology and Molecular Biology, Veterinary Hospital of Federal University of Mato Grosso, Cuiabá, MT, BrazilLaboratory of Veterinary Microbiology and Molecular Biology, Veterinary Hospital of Federal University of Mato Grosso, Cuiabá, MT, Brazil Introduction: Serratia marcescens is an opportunistic pathogen found ubiquitously in the environment and associated with a wide range of nosocomial infections. This multidrug-resistant bacterium has been a cause of concern for hospitals and healthcare facilities due to its ability to spread rapidly and cause outbreaks. Next generation sequencing genotyping of bacterial isolates has proven to be a valuable tool for tracking the spread and transmission of nosocomial infections. This has allowed for the identification of outbreaks and transmission chains, as well as determining whether cases are due to endogenous or exogenous sources. Evidence of nosocomial transmission has been gathered through genotyping methods. The aim of this study was to investigate the genetic diversity of carbapenemase-producing S. marcescens in an outbreak at a public hospital in Cuiaba, MT, Brazil. Methodology: Ten isolates of S. marcenses were sequenced and antibiotic resistance profiles analyzed over 12 days. Results: The isolates were clonal and multidrug resistant. Gentamycin and tigecycline had sensitivity in 90% and 80% isolates, respectively. Genomic analysis identified several genes that encode β-lactamases, aminoglycoside-modifying enzymes, efflux pumps, and other virulence factors. Conclusions: Systematic surveillance is crucial in monitoring the evolution of S. marcescens genotypes, as it can lead to early detection and prevention of outbreaks. https://jidc.org/index.php/journal/article/view/18913Serratia marcescensoutbreakantimicrobial resistancegenomic sequencing |
| spellingShingle | Thalita PPS Cruz Francisco KSF Azevedo Marco A Pepato Paula S Rizzo Francisco JD Souto Bertram Brenig Aristóteles Góes-Neto Siomar C Soares Rommel TJ Ramos Vasco AC Azevedo Mateus M Costa Leonardo Broetto Luciano Nakazato Valéria Dutra Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil Journal of Infection in Developing Countries Serratia marcescens outbreak antimicrobial resistance genomic sequencing |
| title | Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil |
| title_full | Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil |
| title_fullStr | Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil |
| title_full_unstemmed | Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil |
| title_short | Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil |
| title_sort | whole genome sequencing analysis of multidrug resistant serratia marcescens isolates in an intensive care unit in brazil |
| topic | Serratia marcescens outbreak antimicrobial resistance genomic sequencing |
| url | https://jidc.org/index.php/journal/article/view/18913 |
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