Dilution effect of TEMPO-oxidized cellulose nanofibers on reproduction of Daphnia magna
The expanding use of 2,2,6,6-tetramethylpiperidine-1-oxyl radical-oxidized cellulose nanofibers (TEMPO-CNFs) raises concerns about their environmental release and potential ecological impacts. Evaluating their environmental effects is critical for ensuring their sustainable use. In this study, we ai...
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Elsevier
2025-04-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325004373 |
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| author | Hiroyuki Mano Rie Tai Akihiro Moriyama Yoko Iizumi Tomohiko Matsuzawa Toshiya Okazaki Isamu Ogura |
| author_facet | Hiroyuki Mano Rie Tai Akihiro Moriyama Yoko Iizumi Tomohiko Matsuzawa Toshiya Okazaki Isamu Ogura |
| author_sort | Hiroyuki Mano |
| collection | DOAJ |
| description | The expanding use of 2,2,6,6-tetramethylpiperidine-1-oxyl radical-oxidized cellulose nanofibers (TEMPO-CNFs) raises concerns about their environmental release and potential ecological impacts. Evaluating their environmental effects is critical for ensuring their sustainable use. In this study, we aimed to assess the ecotoxicity of TEMPO-CNFs on Daphnia magna reproduction and explore mechanisms underlying observed effects. Reproductive toxicity was evaluated according to the Organisation for Economic Co-operation and Development test guideline, determining the no observed effect concentration (NOEC) and 10 % effect concentration (EC10). Mechanistic insights were investigated through water chemistry analyses, a feeding inhibition test, a gut evacuation experiment, and 10-day life table experiments. The NOEC and EC10 for TEMPO-CNFs were 12.5 mg/L and 3.4 mg/L, respectively. Results supported the dilution-effect hypothesis, indicating reduced availability of essential food, such as Chlorella, as a driver of toxicity. Gut evacuation experiments indicated that TEMPO-CNFs in D. magna individuals were excreted within 24 h when they were transferred to a CNF-free environment. The results suggest that TEMPO-CNFs are unlikely to impact D. magna reproduction unless exposure is continuous and at high concentrations. These findings support the environmentally sustainable application of TEMPO-CNFs with controlled exposure. |
| format | Article |
| id | doaj-art-340d7884b51b4c29bb63aaf736129b5a |
| institution | DOAJ |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-340d7884b51b4c29bb63aaf736129b5a2025-08-20T03:17:35ZengElsevierEcotoxicology and Environmental Safety0147-65132025-04-0129411810110.1016/j.ecoenv.2025.118101Dilution effect of TEMPO-oxidized cellulose nanofibers on reproduction of Daphnia magnaHiroyuki Mano0Rie Tai1Akihiro Moriyama2Yoko Iizumi3Tomohiko Matsuzawa4Toshiya Okazaki5Isamu Ogura6Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan; Corresponding author.Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, JapanResearch Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, JapanNano Carbon Device Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, JapanDepartment of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki, Kagawa 761-0795, JapanNano Carbon Device Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, JapanResearch Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, JapanThe expanding use of 2,2,6,6-tetramethylpiperidine-1-oxyl radical-oxidized cellulose nanofibers (TEMPO-CNFs) raises concerns about their environmental release and potential ecological impacts. Evaluating their environmental effects is critical for ensuring their sustainable use. In this study, we aimed to assess the ecotoxicity of TEMPO-CNFs on Daphnia magna reproduction and explore mechanisms underlying observed effects. Reproductive toxicity was evaluated according to the Organisation for Economic Co-operation and Development test guideline, determining the no observed effect concentration (NOEC) and 10 % effect concentration (EC10). Mechanistic insights were investigated through water chemistry analyses, a feeding inhibition test, a gut evacuation experiment, and 10-day life table experiments. The NOEC and EC10 for TEMPO-CNFs were 12.5 mg/L and 3.4 mg/L, respectively. Results supported the dilution-effect hypothesis, indicating reduced availability of essential food, such as Chlorella, as a driver of toxicity. Gut evacuation experiments indicated that TEMPO-CNFs in D. magna individuals were excreted within 24 h when they were transferred to a CNF-free environment. The results suggest that TEMPO-CNFs are unlikely to impact D. magna reproduction unless exposure is continuous and at high concentrations. These findings support the environmentally sustainable application of TEMPO-CNFs with controlled exposure.http://www.sciencedirect.com/science/article/pii/S0147651325004373Cellulose nanofibrilChronic toxicityCladoceraDaphnidsEcological risk assessmentNanomaterials |
| spellingShingle | Hiroyuki Mano Rie Tai Akihiro Moriyama Yoko Iizumi Tomohiko Matsuzawa Toshiya Okazaki Isamu Ogura Dilution effect of TEMPO-oxidized cellulose nanofibers on reproduction of Daphnia magna Ecotoxicology and Environmental Safety Cellulose nanofibril Chronic toxicity Cladocera Daphnids Ecological risk assessment Nanomaterials |
| title | Dilution effect of TEMPO-oxidized cellulose nanofibers on reproduction of Daphnia magna |
| title_full | Dilution effect of TEMPO-oxidized cellulose nanofibers on reproduction of Daphnia magna |
| title_fullStr | Dilution effect of TEMPO-oxidized cellulose nanofibers on reproduction of Daphnia magna |
| title_full_unstemmed | Dilution effect of TEMPO-oxidized cellulose nanofibers on reproduction of Daphnia magna |
| title_short | Dilution effect of TEMPO-oxidized cellulose nanofibers on reproduction of Daphnia magna |
| title_sort | dilution effect of tempo oxidized cellulose nanofibers on reproduction of daphnia magna |
| topic | Cellulose nanofibril Chronic toxicity Cladocera Daphnids Ecological risk assessment Nanomaterials |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325004373 |
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