An NBS-LRR-encoding gene CsRPM1 confers resistance to the fungus Colletotrichum camelliae in tea plant
Nucleotide binding site, leucine-rich repeat (NBS-LRR) proteins are the main types of disease resistance proteins in plants, which can perceive plant pathogens. Anthracnose, caused by the fungus Colletotrichum camelliae, is one of the most severe diseases in tea plant. Here, we identified an NBS-LRR...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Maximum Academic Press
2023-01-01
|
| Series: | Beverage Plant Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/BPR-2023-0013 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849699716529389568 |
|---|---|
| author | Wuyun Lv Yingjie Xu Hong Jiang Qinghai Cao Xinchao Wang Yuchun Wang |
| author_facet | Wuyun Lv Yingjie Xu Hong Jiang Qinghai Cao Xinchao Wang Yuchun Wang |
| author_sort | Wuyun Lv |
| collection | DOAJ |
| description | Nucleotide binding site, leucine-rich repeat (NBS-LRR) proteins are the main types of disease resistance proteins in plants, which can perceive plant pathogens. Anthracnose, caused by the fungus Colletotrichum camelliae, is one of the most severe diseases in tea plant. Here, we identified an NBS-LRR-encoding gene, CsRPM1, probably conferring resistance of tea plant to C. camelliae. Phylogenetic analysis showed that CsRPM1 was clustered with RPM1 in Arabidopsis and grouped into CC-NBS-LRR (CNL). It contained a signal peptide, a NB-ARC domain, and multiple LRR motifs. RNA-seq and qRT-PCR analysis showed that the transcript level of CsRPM1 was significantly up-regulated after inoculation with C. camelliae. Transiently silencing of CsRPM1 in tea leaves comprised the resistance to C. camelliae, indicating that CsRPM1 was required for plant defense against the pathogen. The subcellular localization showed that CsRPM1 protein was localized in the nucleus, cytoplasm, and cell membrane. Furthermore, the promoter region of CsRPM1 gene contained MeJA-responsive elements, and the expression of CsRPM1 was induced by exogenous methyl jasmonate, suggesting that CsRPM1 gene may be closely related to JA signaling pathway. A total of 17 transcription factors might be responsible for the expression of CsRPM1. Our data indicates that CsRPM1 is required for disease resistance to C. camelliae in tea plant. The characterization of this disease resistance gene sheds light on NLR protein function in tea plant and may facilitate breeding to control the severe anthracnose. |
| format | Article |
| id | doaj-art-33ffced4a65a42c6ad077b654bca8f0c |
| institution | DOAJ |
| issn | 2769-2108 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Beverage Plant Research |
| spelling | doaj-art-33ffced4a65a42c6ad077b654bca8f0c2025-08-20T03:18:31ZengMaximum Academic PressBeverage Plant Research2769-21082023-01-01311710.48130/BPR-2023-0013BPR-2023-0013An NBS-LRR-encoding gene CsRPM1 confers resistance to the fungus Colletotrichum camelliae in tea plantWuyun Lv0Yingjie Xu1Hong Jiang2Qinghai Cao3Xinchao Wang4Yuchun Wang5College of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou 311300, Zhejiang, ChinaCollege of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou 311300, Zhejiang, ChinaCollege of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou 311300, Zhejiang, ChinaCollege of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou 311300, Zhejiang, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, Zhejiang, ChinaCollege of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou 311300, Zhejiang, ChinaNucleotide binding site, leucine-rich repeat (NBS-LRR) proteins are the main types of disease resistance proteins in plants, which can perceive plant pathogens. Anthracnose, caused by the fungus Colletotrichum camelliae, is one of the most severe diseases in tea plant. Here, we identified an NBS-LRR-encoding gene, CsRPM1, probably conferring resistance of tea plant to C. camelliae. Phylogenetic analysis showed that CsRPM1 was clustered with RPM1 in Arabidopsis and grouped into CC-NBS-LRR (CNL). It contained a signal peptide, a NB-ARC domain, and multiple LRR motifs. RNA-seq and qRT-PCR analysis showed that the transcript level of CsRPM1 was significantly up-regulated after inoculation with C. camelliae. Transiently silencing of CsRPM1 in tea leaves comprised the resistance to C. camelliae, indicating that CsRPM1 was required for plant defense against the pathogen. The subcellular localization showed that CsRPM1 protein was localized in the nucleus, cytoplasm, and cell membrane. Furthermore, the promoter region of CsRPM1 gene contained MeJA-responsive elements, and the expression of CsRPM1 was induced by exogenous methyl jasmonate, suggesting that CsRPM1 gene may be closely related to JA signaling pathway. A total of 17 transcription factors might be responsible for the expression of CsRPM1. Our data indicates that CsRPM1 is required for disease resistance to C. camelliae in tea plant. The characterization of this disease resistance gene sheds light on NLR protein function in tea plant and may facilitate breeding to control the severe anthracnose.https://www.maxapress.com/article/doi/10.48130/BPR-2023-0013nbs-lrrcsrpm1defensecolletotrichum camelliaetea plant |
| spellingShingle | Wuyun Lv Yingjie Xu Hong Jiang Qinghai Cao Xinchao Wang Yuchun Wang An NBS-LRR-encoding gene CsRPM1 confers resistance to the fungus Colletotrichum camelliae in tea plant Beverage Plant Research nbs-lrr csrpm1 defense colletotrichum camelliae tea plant |
| title | An NBS-LRR-encoding gene CsRPM1 confers resistance to the fungus Colletotrichum camelliae in tea plant |
| title_full | An NBS-LRR-encoding gene CsRPM1 confers resistance to the fungus Colletotrichum camelliae in tea plant |
| title_fullStr | An NBS-LRR-encoding gene CsRPM1 confers resistance to the fungus Colletotrichum camelliae in tea plant |
| title_full_unstemmed | An NBS-LRR-encoding gene CsRPM1 confers resistance to the fungus Colletotrichum camelliae in tea plant |
| title_short | An NBS-LRR-encoding gene CsRPM1 confers resistance to the fungus Colletotrichum camelliae in tea plant |
| title_sort | nbs lrr encoding gene csrpm1 confers resistance to the fungus colletotrichum camelliae in tea plant |
| topic | nbs-lrr csrpm1 defense colletotrichum camelliae tea plant |
| url | https://www.maxapress.com/article/doi/10.48130/BPR-2023-0013 |
| work_keys_str_mv | AT wuyunlv annbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant AT yingjiexu annbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant AT hongjiang annbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant AT qinghaicao annbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant AT xinchaowang annbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant AT yuchunwang annbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant AT wuyunlv nbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant AT yingjiexu nbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant AT hongjiang nbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant AT qinghaicao nbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant AT xinchaowang nbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant AT yuchunwang nbslrrencodinggenecsrpm1confersresistancetothefunguscolletotrichumcamelliaeinteaplant |